PCR detection of genetically modified maize and soy in mildly and highly processed foods

The Effect of Heat Processing on PCR Detection of Genetically Modified Soy in Bakery Products

“…Since we could not obtain satisfactory amounts of DNA with this protocol when starting with 200 mg of the model cookie, we used the 1000 mg starting material protocol. Similarly, the results from other groups and our previous studies showed that increasing the sample weight allows for the extraction of a sufficient amount of DNA (Ozgen-Arun et al 2013;Vijayakumar et al, 2009). Our study showed that a sufficient amount of DNA could be extracted by using this protocol.…”

“…However, various factors such as the matrix type and processing conditions influence the performance of the extraction methods (Bergerova, et al, 2010;Gryson, 2010;Peanoet al, 2004). At the beginning of this study, we used the cetyltrimethylammonium bromide (CTAB) method, which had been modified to start with 1 g of sample (Ozgen Arun et al, 2013). However, because a quantifiable amount of DNA could not be extracted, we continued to use the Wizard ® Magnetic DNA Purification System for Food.…”

Monitoring the prevalence of genetically modified (GM) soybean in Turkish food and feed products

“…A few other recent reports have also demonstrated the presence of GM soya in foodstuffs in Turkey; Arun et al (2013) reported the presence of CaMV 35S & tNOS in 11/59 (19.3%) of soy-containing food products collected from 2008 to 2011, all of which also tested positive for RRS. CaMV 35S & tNOS were also found in 5/9 soy samples collected in 2009 (Mandaci, Çakir, Turgut-Kara, Meriç, & Ari, 2014).…”

“…But much of this research, especially on soy and maizecontaining products, has focused on the qualitative detection of GM events (Andr eia Z. Dinon, Bosco, & Arisi, 2010;Arun, Yilmaz, & Muratoĝlu, 2013; Bergerov a, Hrn círov a, Stankovsk a, Lopa sovsk a, & Siekel, 2010; Cardarelli, Branquinho, Ferreira, da Cruz, & Gemal, 2005;Elsanhoty, Al-Turki, & Ramadan, 2013;Gürakan, Aydin, & Yilmaz, 2011;Kakihara, Matsufuji, Chino, & Takeda, 2006;Premanandh, Maruthamuthu, Sabbagh, & Al Muhairi, 2012;Zdjelar et al, 2013). The quantitative detection of GM soy events by qPCR has been reported in relatively few countries (Andr eia Zilio Dinon, Treml, de Mello, & Arisi, 2010;Herzallah, 2012;Kim et al, 2013;K€ oppel, van Velsen, Felderer, & Bucher, 2012;Premanandh et al, 2012;Ujhelyi et al, 2008).…”

Multiplex PCR assays for qualitative detection and identification of the GT73, Ms8, Rf3 and T45 varieties of genetically modified oilseed rape

“…Different varieties of polymerase chain reactions are commonly used for monitoring of GMOs in foods and animal feeds (Shin et al, 2013;Kim et al, 2014;Meriç et al, 2014;Datukishvili et al, 2015;Turkec et al, 2016). Nowadays real-time PCR (qPCR) has become a gold standard in the routine analysis of GMOs, allowing very sensitive detection and especially quantification of GMOs in test samples (Dörries et al, 2010;Elsanhoty et al, 2013;Özgen Arun et al, 2013, Zdjelar et al, 2013Fernandes et al, 2014;Kim et al, 2014;SantaMaria et al, 2014;Turkec et al, 2016). However, to quantify GMO content using qPCR, it is necessary to plot standard curves for both the reference gene and transgen, using serial dilutions of DNA extracted from the reference material, which is time-consuming.…”