PCR detection of<i>Salmonella</i>spp. using primers targeting the quorum sensing gene<i>sdiA</i>

Halatsi, Konstantia; Oikonomou, Ioannis; Lambiri, M.; Mandilara, Georgia; Vatopoulos, Alkiviadis; Kyriacou, Adamantini

doi:10.1111/j.1574-6968.2006.00266.x

Cited by 58 publications

(38 citation statements)

References 27 publications

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“…Notably, all the isolates demonstrated positive results on the sdiA gene amplification. sdiA is a quorum sensing gene that encodes a signal receptor of the LuxR family to achieve intercellular signaling for intestinal survival or colonization (Halatsi et al 2006;Walters and Sperandio 2006). Results of 100% prevalence for sdiA and hilA are consistent with the previous findings by Halatsi et al (2006) and Pathmanathan et al (2003) respectively, who reported that sdiA and hilA appeared to be conserved in S. enterica.…”

Section: Wandsworth Wa 1(m) Wa 2(m) Wa 3 (M)supporting

confidence: 84%

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Khoo

Cheah

Lee

et al. 2009

Antonie van Leeuwenhoek

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The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg microl(-1). This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies.

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Section: Wandsworth Wa 1(m) Wa 2(m) Wa 3 (M)supporting

confidence: 84%

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Khoo

Cheah

Lee

et al. 2009

Antonie van Leeuwenhoek

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show abstract

“…PCR amplification was performed using primer pairs described by Bhatta et al (2007), and primer pair sdiA1 and sdiA2 described by Halatsi et al (2006). The amplicon sizes of invA, spvC, and sdiA were 244 bp, 571 bp, and 274 bp, respectively.…”

Section: Detection Of Virulence and Quorum Sensing Genesmentioning

confidence: 99%

Biofilm formation, virulence gene and multi-drug resistance in Salmonella Kentucky isolated in Tunisia

Turki

Ouzari

Mehri

et al. 2012

Food Research International

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“…Primers sdiA1 and sdiA2 (Table 2) were used for Salmonella quorum sensing detection. Amplification was performed in a 25 µl final volume with a reaction mixture containing 0.125 µM of each sdiA primers (Halatsi et al, 2006).…”

Section: Its 16s-23s Pcr and Eric-pcrmentioning

confidence: 99%

“…These biofilms enable Salmonella to survive and spread in the environment outside the host (Janssens et al, 2008). Both biofilm production and pathogenicity are controlled and regulated by so-called quorum sensing (QS) systems (Halatsi et al, 2006;Williams, 2006). It has been reported that Salmonella SdiA detects and responds to AHL signals generated only by other microbial species (Sperandio, 2006;Walters and Sperandio, 2006).…”

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confidence: 99%

Molecular typing, antibiotic resistance, virulence gene and biofilm formation of different Salmonella enterica serotypes

Turki

Mehr

Ouzari

et al. 2014

J. Gen. Appl. Microbiol.

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Salmonella enterica isolates representing commonly isolated serotypes in Tunisia were analyzed using genotyping and phenotyping methods. ERIC and ITS-PCR applied to 48 Salmonella spp. isolates revealed the presence of 12 and 10 different profiles, respectively. The distribution of profiles among serotypes demonstrated the presence of strains showing an identical fingerprinting pattern. All Salmonella strains used in this study were positive for the sdiA gene. Three Salmonella isolates belonging to serotypes Anatum, Enteritidis and Amsterdam were negative for the invA gene. The spvC gene was detected in thirteen isolates belonging to serotypes Anatum, Typhimurium, Enteritidis, Gallinarum and Montevideo. Antibiotic resistance was frequent among the recovered Salmonella isolates belonging to serotypes Anatum, Typhimurium, Enteritidis, Zanzibar and Derby. The majority of these isolates exhibited resistance to at least two antibiotic families. Four multidrug-resistant isolates were recovered from food animals and poultry products. These isolates exhibited not only resistance to tetracycline, sulphonamides, and ampicillin, but also have shown resistance to fluoroquinolones. Common resistance to nalidixic acid, ciprofloxacin and ofloxacin in two S. Anatum and S. Zanzibar strains isolated from raw meat and poultry was also obtained. Furthermore, wastewater and human isolates exhibited frequent resistance to nalidixic acid and tetracycline. Of all isolates, 33.5% were able to form biofilm.Key words: antimicrobial resistance; biofilm; ERIC; ITS; Salmonella enterica; virulence gene Introduction Several species within the genus Salmonella remain a primary cause of gastrointestinal infections worldwide. The pathogenicity of Salmonella depends fundamentally on its virulence factors controlled by chromosomal or plasmidborne determinants. The Salmondlla invasion gene invA is required for invasion into deeper tissue (Swamy et al., 1996). Virulence plasmids are also implicated in survival and multiplication of Salmonella in the reticulo-endothelial system. Both an invasion gene and a virulence plasmid are indispensable for the full expression of virulence. In environmental settings, biofilms represent the common way of life of microorganisms (Latasa et al., 2005). Salmonella is capable for forming biofilms on a variety of biotic and abiotic surfaces. These biofilms enable Salmonella to survive and spread in the environment outside the host (Janssens et al., 2008). Both biofilm production and pathogenicity are controlled and regulated by so-called quorum sensing (QS) systems (Halatsi et al., 2006;Williams, 2006). It has been reported that Salmonella SdiA detects and responds to AHL signals generated only by other microbial species (Sperandio, 2006;Walters and Sperandio, 2006). In response to AHLs, SdiA regulates two Salmonella-specific loci potentially involved in resistance to human complement and intestinal survival or colonization, rck (resistance to complement killing) and srgE (sdiA-regulated gene E).The most commonly incrimi...

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PCR detection ofSalmonellaspp. using primers targeting the quorum sensing genesdiA

Cited by 58 publications

References 27 publications

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Biofilm formation, virulence gene and multi-drug resistance in Salmonella Kentucky isolated in Tunisia

Molecular typing, antibiotic resistance, virulence gene and biofilm formation of different Salmonella enterica serotypes

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