PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails

Cucher, Marcela Alejandra; Carnevale, Silvana; Prepelitchi, Lucila; Labbé, Jorge H.; Wisnivesky‐Colli, Cristina

doi:10.1016/j.vetpar.2005.12.013

Cited by 61 publications

(72 citation statements)

References 18 publications

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“…The enzyme electrophoresis in starch gel is a very cheap and simple biochemical method that could be used in a very Abrous et al (1999Abrous et al ( , 2000, Mage et al (2002) c Vignoles et al (2002) d Barthe (1982, 1987) e Van Aken (1982), Van Aken and Brandt (1987) f Shubkin et al (1992), Rognlie et al (1994Rognlie et al ( , 1996 g Heussler et al (1993), Kaplan et al (1995Kaplan et al ( , 1997 h Krämer and Schnieder (1998), Mostafa et al (2003), Velusamy et al (2004), Cucher et al (2006), Caron et al (2007) i Magalhaes et al (2004) j Heussler et al (1993), Schweizer et al (2007) modest laboratory for a preliminary selection of possible intermediate hosts in a particular epidemiological context but the check out can be difficult (enzyme choice, temperature, chemical revelation). The specificity and sensitivity of the technique is poor when compared with DNA/RNA-based techniques and consequently it is not used anymore.…”

Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

“…To overcome this problem, the PCR mix was modified by the addition of bovine serum albumine (BSA). No amplification was detected with DNA from uninfected, laboratory-reared P. columella and Lymnaea viatrix (Cucher et al 2006). …”

Section: Rna-based Blottingmentioning

confidence: 97%

“…Several research teams used Kaplan's experiments to design PCR protocol and confirm the usefulness of this tool under field or experimental conditions (Krämer andSchnieder 1998, 1999;Krämer 1999;Mostafa et al 2003;Velusamy et al 2004;Caron et al 2007). Additionally, an original PCR protocol using a fragment of the cytochrome C oxidase subunit 1 gene of F. hepatica was compared to microscopic observation in naturally infected snails (Cucher et al 2006).…”

Section: Rna-based Blottingmentioning

confidence: 99%

“…The complete mitochondrial DNA sequence of F. hepatica (GenBank accession number, X15613.1), was used to design the pair of primer used in the next study (Forward 5′-TATGT TTTGATTTTACCCGGG-3′, reverse 5′-ATGAGCAACCA CAAACCATGT-3′) (Cucher et al 2006). The positions of the primers were selected to amplify a 405 bp region of the cytochrome c oxidase subunit 1 gene.…”

Section: Rna-based Blottingmentioning

confidence: 99%

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The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

2008

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In this review, ten methods used to study digenean infections in their intermediate hosts were compared to determine which one should be used either in the field or in the lab to establish the prevalence and intensity of infections in snails. Snail crushing and snail dissection allow quick establishing of prevalence in natural or experimental infections, whereas histology is considered as the most accurate approach to assess the intensity of infection. The follow-up of cercarial shedding only gave an idea on cercarial production. Among recently developed techniques, polymerase chain reaction (PCR) brings the most accurate information and shows high sensitivity and specificity levels when compared to blotting techniques. The easiness and relatively low cost of the basic PCR protocol make it interesting to investigate the epidemiology of the liver fluke in a lab with limited financial resources. Nevertheless, if this technique allows a relatively good estimation of the prevalence, information concerning the intensity of infection is best obtained through real time PCR. However, at the time being this technique is too expensive to be used routinely in the field. The choice between classical or new techniques is usually based on a compromise, as each technique has its advantages and drawbacks.

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Section: Advantages and Disadvantages Of Screening Methodsmentioning

confidence: 99%

Section: Rna-based Blottingmentioning

confidence: 97%

Section: Rna-based Blottingmentioning

confidence: 99%

Section: Rna-based Blottingmentioning

confidence: 99%

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The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

2008

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“…Before cercarial development, however, the intra-molluscan stages of the different trematode species are difficult to differentiate (Kaplan et al, 1995). For this reason, several DNA-or RNA-based techniques were developed (Shubkin et al, 1992;Rognlie et al, 1994;Kaplan et al, 1995;Kramer and Schnieder, 1998;Mostafa et al, 2003;Magalhaes et al, 2004;Cucher et al, 2006). These techniques are specific and sensitive (Kaplan et al, 1997) but are rarely used to detect naturally occurring infections, although this should be one of their main applications.…”

Section: Introductionmentioning

confidence: 99%

Fasciola hepatica: An assessment on the vectorial capacity of Radix labiata and R. balthica commonly found in Belgium

Caron¹,

Lasri²,

Losson³

2007

Veterinary Parasitology

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A previous study conducted in Belgium revealed that genetic material of Fasciola sp. was present in snail species belonging to the genus Radix. Here, these snails were collected and identified by DNA-based techniques as Radix labiata and Radix balthica. These two species and Galba truncatula (the major intermediate host in Europe) were experimentally infected with Fasciola hepatica. The resulting metacercariae were fed to rats and the infection was monitored using several techniques. Microscopy revealed the presence of larval stages in 78.3, 45, and 6.25% of G. truncatula, R. labiata, and R. balthica snails, respectively. These results were confirmed by a PCR that amplifies a Fasciola sp. specific sequence. Furthermore, this PCR was found to be more sensitive than microscopic examination. R. labiata shed fewer metacercariae than G. truncatula but these were as infective to rats as those shed by G. truncatula. This study demonstrates that R. labiata may act as an incidental intermediate host for F. hepatica in Belgium. #

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Lymnaeid Snails and the Transmission of Fasciolosis: Understanding the Differential Risks from Local to Global Scale

Vázquez,

Alba,

Alda

et al. 2023

Zoological Monographs

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PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails

Cited by 61 publications

References 18 publications

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola sp.

Fasciola hepatica: An assessment on the vectorial capacity of Radix labiata and R. balthica commonly found in Belgium

Lymnaeid Snails and the Transmission of Fasciolosis: Understanding the Differential Risks from Local to Global Scale

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