PCR identification of Salmonella serogroups based on specific targets obtained by comparative genomics

Liu, Bin; Zhang, Lida; Zhu, Xun; Shi, Chunlei; Chen, Jing; Liu, Weibing; He, Xiaohua; Shi, Xianming

doi:10.1016/j.ijfoodmicro.2010.11.010

Cited by 40 publications

(27 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The wzx C1 gene, which is located in the O-antigen gene cluster, was identified in our previous work as being specific for Salmonella serogroup C1 [18]. In the present study, we provide experimental evidence to reveal the relationship between this gene and the O-polysaccharide biosynthesis of serogroup C1.…”

Section: Discussionsupporting

confidence: 51%

See 1 more Smart Citation

Mutation of a Salmonella Serogroup-C1-Specific Gene Abrogates O7-Antigen Biosynthesis and Triggers NaCl-Dependent Motility Deficiency

et al. 2014

Self Cite

View full text Add to dashboard Cite

Several molecular detection marker genes specific for a number of individual Salmonella serogroups have been recently identified in our lab by comparative genomics for the genotyping of diverse serogroups. To further understand the correlation between serotype and genotype, the function of a Salmonella serogroup-C1-specific gene (SC_2092) was analyzed in this study. It was indicated from the topological prediction using the deduced amino acid sequence of SC_2092 that this putative protein was highly similar to the confirmed Wzx flippases. Furthermore, SDS-PAGE revealed that lipopolysaccharide (LPS) biosynthesis, specifically O-antigen synthesis, was incomplete in an SC_2092 in-frame deletion mutant, and no agglutination reaction with the O7 antibody was exhibited in this mutant. Therefore, it was revealed that this Salmonella serogroup-C1-specific gene SC_2092 encoded a putative flippase, which was required for O7-polysaccharide biosynthesis, and was designated here as wzxC1. Subsequently, the effects of the deletion of wzxC1 on bacterial motility and sodium chloride (NaCl) tolerance were evaluated. The wzxC1 mutant lacked swarming motility on solid surfaces and was impaired in swimming motility in soft agar. Moreover, microscopic examination and RT-qPCR exhibited that an increased auto-aggregation and a strong defect in flagella expression, respectively, were responsible for the reduced motility in this mutant. In addition, the wzxC1 mutant was more sensitive than the wild-type strain to NaCl, and auto-aggregation of mutant cells was observed immediately up on the addition of 1% NaCl to the medium. Interestingly, the motility deficiency of the mutant strain, as well as the cell agglomeration and the decrease in flagellar expression, were relieved in a NaCl-free medium. This is the first study to experimentally demonstrate a connection between a Salmonella serogroup specific gene identified by comparative genomics with the synthesis of a specific O-antigen biosynthesis. Also, our results show that the mutation of wzxC1 triggers a NaCl-dependent motility deficiency.

show abstract

Section: Discussionsupporting

confidence: 51%

“…Recently in our lab, we found seven genes that were conserved and specific for Salmonella serogroup C1 by comparative genomic analysis [18]. According to preliminary analyses, most of the C1-specific genes encode membrane proteins with high numbers of transmembrane segments (TMs).…”

Section: Introductionmentioning

confidence: 99%

Mutation of a Salmonella Serogroup-C1-Specific Gene Abrogates O7-Antigen Biosynthesis and Triggers NaCl-Dependent Motility Deficiency

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…The fragments which were not present in the genome sequences of the 17 strains with an E-value more than 10 À2 were identified as the specific sequences for S. Typhimurium. The method of comparative genomics was described by Chen et al (2010) and Liu et al (2011). These serovar-specific fragments were considered as candidate targets for PCR.…”

Section: Screening Of Salmonella Serovar-specific Detection Targetsmentioning

confidence: 99%

Development of a novel multiplex PCR assay for the identification of Salmonella enterica Typhimurium and Enteritidis

et al. 2012

Self Cite

View full text Add to dashboard Cite

“…Currently, alternative molecular typing methods such as multilocus sequence typing (MLST) and virulotyping [1,[14][15] are used to characterize many Salmonella enterica serovars. Multilocus sequence typing [16] has been increasingly recognized as a method of choice for genotyping a number of bacterial pathogens [17][18], including Salmonella [1,[19][20][21], and has been used successfully in epidemiological studies and outbreak investigations [17,22]. This technique is based on determination of the DNA sequence of a series of selected housekeeping, ribosomal, and/or virulenceassociated genes [23][24].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and molecular analysis of Salmonella serovar Livingstone strains isolated from humans in Tunisia and Belgium

Guedda

Taminiau

Ferjani

et al. 2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Salmonella Livingstone is one of the most common serotypes responsible for nosocomial outbreaks in Tunisia. In this study, 42 isolates of Salmonella Livingstone were analyzed. Most of these were isolated from humans (31 strains from Tunisia and 9 strains from Belgium) and 2 isolates came from food products (beef and pork). Methodology: All strains were characterized by antibiogram, multilocus sequence typing (MLST), and virulotyping. This last technique was carried out by simple PCR of five chromosomal genes (agfA, hin/H2, iroB, phoP/Q, and slyA) and two plasmid genes (spvA and spvC). Results: All Tunisian strains were resistant to amoxicillin, amoxicillin-clavulanic acid, ticarcillin, cefalotin, gentamicin, and kanamycin. They were also resistant to third-generation cephalosporin antibiotics (cefotaxim and ceftazidim). Belgian isolates were susceptible to all antibiotics tested. Further to MLST analyses, Tunisian strains belonged to the same sequence type, ST543. For Belgian isolates, eight strains had a ST543 profile, two strains had a ST638 profile, and one strain had a ST457 profile. Analyses of the virulence gene contents showed that strains isolated in different years and from different origins had the same virulence profile. These carried all five chromosomal genes and lacked plasmid-located virulence genes spvA and spvC. Conclusions: A combination of different typing methods showed that the majority of Belgian strains and all Tunisian strains were closely related; they belonged to the same sequence type (ST543) and had the same virulence profile, but different antibiotic resistance profiles depended on the country of origin.

show abstract

PCR identification of Salmonella serogroups based on specific targets obtained by comparative genomics

Cited by 40 publications

References 36 publications

Mutation of a Salmonella Serogroup-C1-Specific Gene Abrogates O7-Antigen Biosynthesis and Triggers NaCl-Dependent Motility Deficiency

Mutation of a Salmonella Serogroup-C1-Specific Gene Abrogates O7-Antigen Biosynthesis and Triggers NaCl-Dependent Motility Deficiency

Development of a novel multiplex PCR assay for the identification of Salmonella enterica Typhimurium and Enteritidis

Antimicrobial and molecular analysis of Salmonella serovar Livingstone strains isolated from humans in Tunisia and Belgium

Contact Info

Product

Resources

About