PCR in lyme neuroborreliosis: a prospective study

Pícha, Dušan; Moravcová, Lenka; Zdárský, E; Marešová, Vilma; Hulínský, V.

doi:10.1111/j.1600-0404.2005.00482.x

Cited by 20 publications

(11 citation statements)

References 33 publications

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“…This value correlates with previously reported results: 18.4% of PCR-positive plasma samples from patients with EM (Goodman et al, 1995) and 28% of PCR-positive plasma samples from patients with active neuroborreliosis (Picha et al, 2005). It should be mentioned that it was not always possible to detect Borrelia DNA in blood by PCR, especially if relatively small amounts of human blood were examined in a single round of PCR, or analyzed blood of patients with post-Lyme borreliosis syndromes, or isolated DNA from small quantities of plasma (Klempner et al, 2001;Coulter et al, 2005).…”

Section: Discussionsupporting

confidence: 93%

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Фоменко¹,

Ливанова²,

Chernousova³

2008

International Journal of Medical Microbiology

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Ixodes persulcatus ticks, wild small mammals, and infected people in Novosibirsk region of Russia were investigated to analyze the local distribution and diversity of Borrelia burgdorferi sensu lato. The molecular typing was based on the partial sequencing of the 16S rRNA gene and the 5S-23S rRNA intergenic spacer region. The analysis of our data revealed the presence of two Borrelia species, B. garinii and B. afzelii. B. garinii DNA was found much more often, namely in 74% of all PCR-positive human blood samples, in 72% of all samples from small mammals (including blood and tissues), and in 76% of Borrelia-positive unfed adult I. persulcatus. B. afzelii DNA was found in 21% of the PCR-positive human blood samples, in 20% of the PCR-positive samples from the small mammals (including blood and tissues) and in 19% of the PCR-positive ticks. Mixed infection with B. garinii and B. afzelii was observed in both clinical and environmental samples. Interestingly, four samples isolated from I. persulcatus were closely related to the relapsing fever species B. miyamotoi.

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Section: Discussionsupporting

confidence: 93%

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Фоменко¹,

Ливанова²,

Chernousova³

2008

International Journal of Medical Microbiology

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“…It is possible to draw attention to the high number of positive PCR examined in urine. The safety of this material for DNA testing is often questioned in many reports, but some previous and recent results provide a good promise for the future 16,19,26,32 …”

Section: Discussionmentioning

confidence: 99%

“…We have already studied this problem earlier, 19 and in this study an attempt was made to check the new arrangement PCR in a group of patients with different forms of LB. The main aim of study was the clinical testing of PCR with an emphasis on the reactivity of the newly selected primers in different forms of LB.…”

Section: Introductionmentioning

confidence: 99%

Examination of specific DNA by PCR in patients with different forms of Lyme borreliosis

et al. 2008

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“…Results of PCR-based detection of B. burgdorferi in CSF have been variable at best. Some reports suggest it may be useful in acute meningitis; generally, this approach is probably best reserved for research studies at this time [8,[41][42][43]. Some laboratories have used unconventional techniques.…”

Section: Diagnosismentioning

confidence: 96%

Neurologic Manifestations of Lyme Disease

Halperin

2011

Curr Infect Dis Rep

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Borrelia burgdorferi infection, the tick-borne spirochetosis known as Lyme disease or Lyme borreliosis, involves the nervous system (neuroborreliosis) in 10% to 15% of patients. Common manifestations include lymphocytic meningitis, cranial neuritis, mononeuropathy multiplex, and painful radiculoneuritis. Rare patients develop inflammation in the brain or spinal cord. Regardless of the form of involvement, neuroborreliosis can be microbiologically cured in virtually all patients using standard 2- to 4-week antimicrobial regimens. Oral regimens appear to be as effective as parenteral ones in most instances. Although patients ill with Lyme disease may have concomitant cognitive or memory difficulty, these symptoms are not specific to neuroborreliosis and, when present in isolation, should not be viewed as suggestive of this diagnosis. When present as part of Lyme disease, they do not require additional or different treatment.

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PCR in lyme neuroborreliosis: a prospective study

Abstract: The highest sensitivity of PCR was achieved in the acute period of neuroborreliosis - 63.1% in three body fluids comparing with CSF antibody synthesis.

Cited by 20 publications

References 33 publications

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Diversity of Borrelia burgdorferi sensu lato in natural foci of Novosibirsk region

Examination of specific DNA by PCR in patients with different forms of Lyme borreliosis

Neurologic Manifestations of Lyme Disease

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