PCR-ITS-RFLP identification of pineapple spoilage fungi

Yang

et al. 2020

BMC Oral Health

Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87, 18.75, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. S AB were 100%, and inter-groups similarity coefficients, i.e. S AB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.

Section: Introductionmentioning

confidence: 99%

A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

Yang

et al. 2020

BMC Oral Health

“…The IRB numbers for our medical ethic files were No. 2015 (17) of the stomatology hospital and 2010 (137) of the 2 nd hospital affiliated to Medical School Zhejiang University. All participants underwent training session to minimize the undesirable discrepancy.…”

Section: Methodsmentioning

confidence: 99%

A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

Yang

et al. 2019

Preprint

Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods:Positive rates and genotypic profiles of Candida albicansstrains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNApolymerase chain reactions were used to sequence the DNA of these strains, and then their geneticsimilarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence AnalysesSoftware. Results:The prevalence of C. albicansstrains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SABwere 100%, and inter-groups similarity coefficients, i.e. SABwere less than 30%. Conclusions:The genotypic results of C. albicansin OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicansin OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.

“…The IRB numbers for our medical ethic files were No. 2015 (17) of the stomatology hospital and 2010 (137) of the 2nd hospital affiliated to Medical School Zhejiang University. Training session was brought forward to all participants to minimize the undesirable discrepancy.…”

Section: Methodsmentioning

confidence: 99%

“…Combination of random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) [17][18][19] of ribosome DNA polymerase chain reaction (PCR)was used to intergroup genotype C. albicans isolates from healthy individuals and OLP patients. The results showed that the phenotypes and intragroup genotype of C. albicans were homologous but the genotypes of C. albicans were heterogeneous among erosive(E)-OLP, non-erosive (NE)-OLP and healthy individuals.…”

mentioning

confidence: 99%

A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

Zhang

et al. 2019

Preprint

Objectives：This study aimed to investigate the prevalence and genotypic profiles of Candida albicans from patients with oral lichen planus (OLP). Materials andMethods：Genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reaction were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results：The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients S AB were 100%, and inter-groups similarity coefficients S AB were less than 30%. Conclusions：The genotypic evidence of C. albicans in OLP might inferred an endogenous infection and some etiologic sense contributing to professional recognition on the development and progression of OLP for more suitable diagnose and treatment.