PCR METHOD FOR THE DETECTION OF GENUS FUSARIUM AND FUMONISIN‐PRODUCING ISOLATES FROM FRESHLY HARVESTED SORGHUM GRAINS GROWN IN KARNATAKA, INDIA

Abstract

Sixty‐four isolates of Fusarium species isolated from 44 sorghum samples collected during 2004–2005 were subjected to polymerase chain reaction (PCR) analysis. PCR detection was performed on all Fusarium species using two different sets of primers, namely, internal transcribed spacer (ITS) and FUM1. The developed protocol is rapid for small‐scale extraction of DNA from fungal mycelia for the detection of Fusarium species using diagnostic PCR. Sixty‐four Fusarium isolates, namely, Fusarium verticillioides (45), Fusarium proliferatum (4), Fusarium anthophilum (4), Fusarium sporotrichioides (3), Fusarium pallidoroseum (6) and Fusarium oxysporum (2), were analyzed by PCR using the ITS and FUM1 set of primers. All Fusarium species scored positive with the ITS set of primers. Among the 64 isolates, 53 have scored positive with the FUM1 set of primers. These 53 isolates represent F. verticillioides (45), F. proliferatum (4) and F. anthophilum (4), respectively. The results of the study revealed that PCR‐based technique could be used to identify a group of potential fumonisin‐producing Fusarium species. PRACTICAL APPLICATIONS Polymerase chain reaction (PCR) method provides the basis for a simple, accurate, rapid and precise detection of potential fumonisin producing Fusarium species, which are of considerable risk to animal and human health. The detection of Fusarium species is therefore crucial for the prevention of toxins entering the food chain. The protocols developed in this study are helpful for a rapid and small‐scale extraction of nucleic acid from fungal mycelia, occurring on contaminated sorghum samples. A large number of samples can be screened in relatively less time using this PCR protocol when compared with the conventional methods.

Morphological and molecular identification and PCR amplification to determine the toxigenic potential of Fusarium spp. isolated from maize ears in southern Poland

“…In this study, only three potential fumonisin producers could be identified. This result is surprising, as it is in contradiction with the data of Sreenivasa et al (2008) and Yazeed et al (2011), in which 53 of 64 plant-derived isolates and all of the 21 feed-samples-derived isolates, respectively, were identified as fumonisin producers. The results of the biological test are similar to the ones obtained by Bis (2006) in the analysis of potentially toxicogenic fungal strains isolated from soils in Cracow and its surroundings, where only 60% of the isolated strains could have been considered as toxic.…”

“…isolates was analyzed in accordance with the PCR-based approach with FUM1-F and FUM1-R primer pair introduced by Sreenivasa et al (2008). In this study, only three potential fumonisin producers could be identified.…”

Assessment of the growth inhibiting effect of some plant essential oils on different Fusarium species isolated from sorghum and maize grains

“…All Fusarium isolates were identified up to species level based on the micro-morphological features, using fungal keys (Leslie & Summerell 2006). The identity was confirmed by PCR, using Fusarium genus specific primer sequences (Sreenivasa et al 2008).…”

DETECTION OF FUMONISIN PRODUCING STRAINS OF Fusarium moniliforme CAUSING RICE BAKANAE DISEASE USING MOLECULAR MARKERS

“…Previous workers (Baird et al, 2008 andSrinivasa et al, 2008) used this primer only with maize infecting Fusarium tissues. This study is the first effort on rice in Egypt represented banding size for both VERTF and Fum 5 primers.…”