PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

Theune, Sheila M.; Fung, Jennifer C.; Todd, S.; Sakaguchi, Alan Y.; Naylor, Susan L.

doi:10.1016/0888-7543(91)90418-e

Cited by 49 publications

(21 citation statements)

References 47 publications

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“…To identify those human chromosome(s) mandatory for PAg presentation, hybrid cells were cloned and tested for induction of reporter cell stimulation in the presence of 1 nM HMBPP. PCR karyotyping showed that loss of human chromosomes 2,3,7,8,9,10,11,13,17,18,20,21, and X had no effect on PAg-mediated activation of the reporter cells, while cells without Chr6 failed to induce activation of the reporter cells in the presence of HMBPP or zoledronate. For reasons so far unknown, 2 of 6 of the Chr6-bearing hybridoma cell lines stimulate the reporter cells in the presence of HMBPP and zoledronate but not in the presence of 2 mM sec-butylamine.…”

Section: Resultsmentioning

confidence: 99%

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Vγ9Vδ2 TCR‐activation by phosphorylated antigens requires butyrophilin 3 A1 (BTN3A1) and additional genes on human chromosome 6

et al. 2014

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Section: Resultsmentioning

confidence: 99%

“…Mouse-human hybridoma cells were karyotyped by PCR [17,18] with parental lines as reference. Content of human genes in CHO Chr6 cells was confirmed by PCR karyotyping [17,18].…”

Section: Pcr-karyotypingmentioning

confidence: 99%

Vγ9Vδ2 TCR‐activation by phosphorylated antigens requires butyrophilin 3 A1 (BTN3A1) and additional genes on human chromosome 6

et al. 2014

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“…To verify the identity of the human chromosome, PCR analysis of some hybrids was also carried out with primers specific for this chromosome (28,31). These data are summarized in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2.

Jeggo

Hafezparast

Thompson

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Complementation of the repair defect in hamster xrs mutants has been achieved by transfer of human chromosome 2 using the method of microcell-mediated chromosome transfer. The xrs mutants belong to ionizing radiation complementation group 5, are highly sensitive to ionizing radiation, and have an impaired ability to rejoin radiation-induced DNA double-strand breaks. Both phenotypes were corrected by chromosome 2, although the correction of radiation sensitivity was only partial. Complementation was achieved in two members of this complementation group, xrs6 and XR-V15B, derived independently from the CHO and V79 cell lines, respectively. The presence of human chromosome 2 in complemented clones was examined cytogenetically and by PCR analysis with primers directed at a human-specific long interspersed repetitive sequence or chromosome 2-specific genes. Complementation was observed in 25/27 hybrids, one of which contained only the q arm of chromosome 2. The two noncomplementing hybrids were missing segments of chromosome 2. The use of a back-selection system enabled the isolation of clones that had lost the human chromosome and these regained radiation sensitivity. Transfer of several other human chromosomes did not result in complementation of the repair defect in XR-V15B. These data show that the gene defective in xrs cells, XRCC5, which is involved in double-strand break rejoining, is located on human chromosome 2q.

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“…The Hs27 (newborn human foreskin) cell line was obtained from the ATCC (CRL 1634). Primers for detecting the human 32-microglobulin gene (B2M) were B2MI (5'-CAC-CCAGTCTAGTGCATGCCTTCT-3') and B2M2 (5'-TGAGAAGGAAGTCACGGAGCGAGA-3') and for detecting the human gastrin gene (GAS) were GAS1 (5'-ATGCTAGTCG-GTGTAGAGCCATG-3') and GAS2 (5'-TTGTACCTCAT-AGGGCTGCGTGA-3') (27).…”

Section: Methodsmentioning

confidence: 99%

Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast.

Lee¹,

Peterson²,

Calman³

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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A human cDNA encoding a galactokinase (EC 2.7.1.6) was isolated by complementation of a galactokinase-deficient (gal1-) strain of Saccharomyces cerevisiae. This cDNA encodes a predicted protein of 458 amino acids with 29% identity to galactokinase of Saccharomyces carlsbergensis. Previous studies have mapped a human galactokinase gene (GK1) to chromosome 17q23-25, closely linked to thymidine kinase. The galactokinase gene that we have isolated (GK2) is located on chromosome 15. The relationship between the disease locus for galactokinase deficiency galactosemia, which is responsible for cataracts in newborns and possibly presenile cataracts in adults, and the two galactokinase loci is unknown.

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PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

Cited by 49 publications

References 47 publications

Vγ9Vδ2 TCR‐activation by phosphorylated antigens requires butyrophilin 3 A1 (BTN3A1) and additional genes on human chromosome 6

Vγ9Vδ2 TCR‐activation by phosphorylated antigens requires butyrophilin 3 A1 (BTN3A1) and additional genes on human chromosome 6

Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2.

Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast.

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