PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae)

Abstract

ABSTRACT. PCR-RAPD and PCR-RFLP polymorphisms detected in Anopheles cruzii (Diptera, Culicidae). The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the

Assessing the molecular divergence between Anopheles (Kerteszia) cruzii populations from Brazil using the timeless gene: further evidence of a species complex

“…Although, they found some differences between sequences from different localities, including Juquitiba and Cananéia, they considered premature to conclude based on their results that there are distinct sibling species in the areas they investigated. Similar results were observed by Calado et al [ 44 ] using PCR-RAPD and PCR-RFLP of the ITS2 region.…”

Speciation in Brazilian Atlantic Forest Mosquitoes: A Mini-Review of the Anopheles cruzii Species Complex

“…cruzii populations from Brazil. Similar results were observed by Calado et al (2006), using PCR-RAPD and PCR-RFLP of the ITS2 region.…”

“…cruzii (e.g. Calado et al, 2006;Carvalho-Pinto & Lourenço-de-Oliveira, 2004;Malafronte et al, 2007;Ramirez & Dessen, 2000a,b; see also below).…”

Kerteszia subgenus of Anopheles associated with the Brazilian Atlantic rainforest:current knowledge and future challenges

“…homunculus using ITS2 RFLP, indicating that this could be used as a tool to distinguish between these species. It should be noted that none of the ITS2 sequences generated by Calado et al [52] and Calado and Navarro-Silva [53] have been deposited in GeneBank, preventing further analyses of the sequences used in both studies.…”