PCR‐RFLP Analysis of Mitochondrial DNA for Identification of Snail Meat Species

Abstract

Several species of land snails, including Helix pomatia and Helix lucorum are consumed as food products. The main source of commercial competition is an imported African snail, Achatina fulica. The only way to distinguish between these species has been morphologically. We hypothesized a reliable method for identifying canned snails could be based on using PCR‐RFLP analysis of mito‐chondrial DNA. The molecular weights of the amplified fragments were perfectly identical, regardless of low extraction (fresh snails or cooked and canned samples). The whole amplified products (16S rRNA and 12S rRNA) made it possible to check any fraudulent label and identify the three species using four restriction enzymes (RFLP).

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

“…DdeI helped in differentiation of chital and sambar from each other. Also, 16S rRNA was used for species identification by PCR-RFLP method (Borgo et al, 1996;Sawyer et al, 2003). Chikuni et al (1994) discriminated goat and sheep meat by PCR-RFLP of satellite I DNA sequence using restriction enzyme of ApaI.…”

PCR-RFLP of the mitochondrial cytochrome oxidase gene: a simple method for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)

“…However, while sequencing speci®c DNA regions is a valuable technique in phylogenetic and population studies 24±26 it is costly and time-consuming and might be inappropriate for food analysis of a large number of samples. Several authors 14,21,22,27,28 have determined the discriminatory power of restriction fragment length polymorphisms analysis of amplicons (PCR-RFLP) obtained from mitochondrial DNA for animal species identi®cation. With this approach, amplicons are not sequenced but are digested with restriction endonucleases.…”

Analytical Methods for Quantification of Relative Flying Fish Paste Content in Processed Sea Food (ago-noyaki)

“…In particular, analysis of mitochondrial DNA (mtDNA), which has species-specific features in the base sequences and a high genetic stability due to maternal inheritance, has been used widely in the study of phylogeny and population structure of fishes and in the authentication of fish products. Complete mtDNA sequences of many fish species are already available in DNA databases, i.e., GenBank, EMBL, DDBJ and MitBASE (Borgo et al, 1996, Attimonelli et al, 2000, Lockley and Bardsley., 2000, Inoue et al, 2000, Inoue et al, 2001a, Inoue et al, 2001b., Ishiguro et al, 2001, Miya et al, 2000, Manchado et al, 2004, Yanagimoto et al, 2004, Mabuchi et al, 2007. Thus, mtDNA analysis is useful for authentication of ingredients of commercial fishery products.…”

“…Such authentication of agonoyaki products is necessary to ensure the proper use and quality of flying fish and to eliminate imitation flying fish paste products from local markets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) has been widely used for authentication of fish species, such as Alaska pollack (Aranishi et al, 2005), tuna (Ram et al, 1996, Lin andHwang, 2007), snail (Borgo et al, 1996), bonito (Ram et al, 1996), and Atlantic mackerel (Infante et al, 2006).…”