PCR–RFLP analysis of the dermatophytes isolated from patients in Central Poland

Dobrowolska, Anita; Stączek, Paweł; Kaszuba, Aleksandra; Kozłowska, Magdalena

doi:10.1016/j.jdermsci.2006.01.001

Cited by 30 publications

(28 citation statements)

References 5 publications

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“…2,3 Dermatophytes are a group of closely related keratinophilic fungi that can invade keratinized tissues of humans and animals such as skin, hair and nails causing dermatophytosis. 4 Dermatophytosis includes several distinct clinical manifestations. The severity of the disease depends on strain or species of infecting fungus, the sensitivity of the host and the site of infection.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Dermatophytic Infection and Detection of Dermatophytes by Microscopic and Culture Methods

Islam

Majid

Ahmed³

et al. 2018

J Enam Med Col

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Section: Introductionmentioning

confidence: 99%

Prevalence of Dermatophytic Infection and Detection of Dermatophytes by Microscopic and Culture Methods

Islam

Majid

Ahmed³

et al. 2018

J Enam Med Col

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“…These difficulties in comparing a new technique to reference mycological methods that have their own limitations have already been underlined (3,13). The poor sensitivity and specificity of direct examination (10) and the fact that culture is negative in a significant percentage of cases of true dermatophytic onychomycosis, despite positive direct microscopy, could account for the difference in sensitivity/specificity between PCR and mycology.…”

Section: Discussionmentioning

confidence: 99%

“…Most are based on real-time PCR, species-specific nested PCR, or a combination of PCR with restriction fragment length polymorphism or sequencing (3,9,11,12,13,16,18,19). Menotti et al (17) previously showed the excellent sensitivity and specificity of a PCR-restriction fragment length polymorphism assay for rapid diagnosis of dermatophytes and Scytalidium spp.…”

mentioning

confidence: 99%

Multicenter Evaluation of a Commercial PCR-Enzyme-Linked Immunosorbent Assay Diagnostic Kit (Onychodiag) for Diagnosis of Dermatophytic Onychomycosis

Savin¹,

Huck

Rolland

et al. 2007

J Clin Microbiol

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We prospectively evaluated a new PCR-enzyme-linked immunosorbent assay kit (Onychodiag; BioAdvance, France) for the diagnosis of dermatophytic onychomycosis by testing nail samples from 438 patients with suspected onychomycosis and from 108 healthy controls in three independent laboratories. In two laboratories, samples were collected by trained mycologists as close as possible to the lesions (proximal samples). In one laboratory, samples were collected by other physicians. All samples were processed by conventional mycological techniques and by Onychodiag, blindly to the mycological results. An additional distal sample, collected by clipping the nail plate, was obtained from 75 patients and tested with Onychodiag alone. In patients with culture-proven dermatophytic onychomycosis, the sensitivity of Onychodiag was 83.6% (87.9% including the gray zone) and ranged from 75 to 100% according to the laboratory and the sampling conditions. The specificity was 100% when healthy subjects were considered true negative controls. Onychodiag was positive on 68 patient samples that were sterile or yielded nondermatophyte species in culture. Based on the results of Onychodiag for mycologically proven positive samples and true-negative samples, these results were considered true positives, and the poor performance of mycology on these samples was attributed to inconvenient sampling conditions or to contaminants. When tested on distal samples, Onychodiag was positive in 49/53 (92%) cases of proven dermatophytic onychomycosis. Finally, with either proximal or distal samples, Onychodiag provided a diagnosis of dermatophytic onychomycosis within 24 to 48 h after sampling, and its sensitivity was close to that of mycological techniques applied to proximal samples.

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“…Some species were isolated only from one substrate and not from the other such as C. asperatium from goat and C. pannorum from sheep hairs ( Table 1). Most of these fungi were recovered, with variable degrees and densities from animals hair or natural soil baited, with sterilized human or animals hair from different parts of the world (El-Said, 2002;Periasamy et al, 2004;Dobrowolska et al, 2006;Yahyaraeyat et al, 2009;Beraldo et al, 2011;Emenuga and Oyeka, 2013;Enany et al, 2013;Debnath et al, 2015).…”

Section: Dermatophytic and Keratinophilic Fungi (Using Hair Baiting Tmentioning

confidence: 99%

“…The presence of keratinophilic fungi on hairs of various animals has been briefly reviewed by numerous researchers in many parts of the world (Ali-Shtayeh et al, 2000;Dobrowolska et al, 2006;Al-Duboon and Farhan, 2007;Nichita and Marcu, 2010;Sallam and ALKolaibe, 2010;Jain and Sharma, 2012;Emenuga and Oyeka, 2013;Enany et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Awad¹

2017

Afr. J. Microbiol. Res.

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The objective of this study was to evaluate the occurrence of mycoflora in 30 samples of healthy goat's hair and sheep wool collected from different localities in Taif, Saudi Arabia, and the ability of some fungal isolates for keratinase activities. Sixty four species belonging to 28 genera were collected from the two substrates. The wool of sheep was polluted with fungi than goat hairs, and contained high total counts and number of fungal genera and species. Nine species of true dermatophytes isolated belonged to Microsporum (3 species) and Trichophyton (6 species). Several keratinophilic species were isolated of which, Chrysosporium indicum, Chrysosporium keratinophilum and Chrysosporium tropicum were the most prevalent. The commonest saprophytes in order of frequency were members of the genera, Aspergillus, Penicillium, Alternaria and Cochliobolus. In addition, the other genera found included Acremonium, Chaetomum, Cladosporium, Cochliobolus, Fusarium, Mucor, Paecilomyces, Phoma, Rhizopus, Scopulariopsis, Stachybotrys, Trichoderma and others. Five species from 20 tested isolates (Aspergillus niger, C. keratinophilum, C. tropicum, Microsporum gypseum and Trichoderma viride) had high keratinase activity. The results of this study indicate that both goat hair and sheep wool provide a suitable habitat for dermatophytes and other keratinophilic fungi. Most of these fungi play an important role in the degradation of keratin substrates, so that they can help preserve the environment and reduce pollution.

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PCR–RFLP analysis of the dermatophytes isolated from patients in Central Poland

Cited by 30 publications

References 5 publications

Prevalence of Dermatophytic Infection and Detection of Dermatophytes by Microscopic and Culture Methods

Prevalence of Dermatophytic Infection and Detection of Dermatophytes by Microscopic and Culture Methods

Multicenter Evaluation of a Commercial PCR-Enzyme-Linked Immunosorbent Assay Diagnostic Kit (Onychodiag) for Diagnosis of Dermatophytic Onychomycosis

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

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