PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil

Silveira, Ana Paula Stefanello da; Vieira, Victor Bruno Duarte; Batalini, Leticia Surian; Carmo, Silvia Barbosa do; Friozi, Elisabete; Arruda, Eduardo José de; Lima, Manoel Sebastião da Costa; Neitzke-Abreu, Herintha Coeto

doi:10.1590/0037-8682-0040-2018

Cited by 12 publications

(8 citation statements)

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“…co-seropositivity was high (41%) and higher than those reported in studies carried out on dogs from Cyprus (36.2%) PLOS ONE [39], Côte d'Ivoire (4.9%) [12], and Portugal (2.2%) [40]. This frequency was also higher than those found in dogs from Campo Grande, Mato Grosso do Sul (31.7%) [41], and Cuiabá, Mato Grosso (2.5%) [42], Brazil. On the other hand, higher frequencies of L. infantum and Ehrlichia spp.…”

Section: Discussionmentioning

confidence: 52%

Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum

et al. 2021

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In canine leishmaniosis caused by the protozoan Leishmania infantum, little is known about how co-infections with or co-seropositivities for other pathogens can influence aggravation of this disease. Therefore, the objectives of this study were to evaluate the frequency of co-infections with or co-seropositivities for certain pathogens in dogs seropositive for L. infantum and their relationship with clinical signs, histological changes and L. infantum load. Sixty-six L. infantum-seropositive dogs were submitted to clinical examination, collection of blood and bone marrow, culling, and necropsy. Antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato, Ehrlichia spp. and Toxoplasma gondii and Dirofilaria immitis antigens were investigated in serum. Samples from different tissues were submitted to histopathology and immunohistochemistry for the detection of Leishmania spp. and T. gondii. Quantitative real-time PCR was used to assess the L. infantum load in spleen samples. For detection of Coxiella burnetii, conventional PCR and nested PCR were performed using bone marrow samples. All 66 dogs tested positive for L. infantum by qPCR and/or culture. Fifty dogs (76%) were co-seropositive for at least one pathogen: T. gondii (59%), Ehrlichia spp., (41%), and Anaplasma spp. (18%). Clinical signs were observed in 15 (94%) dogs monoinfected with L. infantum and in 45 (90%) dogs co-seropositive for certain pathogens. The L. infantum load in spleen and skin did not differ significantly between monoinfected and co-seropositive dogs. The number of inflammatory cells was higher in the spleen, lung and mammary gland of co-seropositive dogs and in the mitral valve of monoinfected dogs. These results suggest that dogs infected with L. infantum and co-seropositive for certain pathogens are common in the region studied. However, co-seropositivities for certain pathogens did not aggravate clinical signs or L. infantum load, although they were associated with a more intense inflammatory reaction in some organs.

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Section: Discussionmentioning

confidence: 52%

Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum

et al. 2021

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“…The MC-PCR methodology used in the present work was performed with primers MC1 and MC2 that were designed specifically for the species L. donovani and L. infantum [1,4,18] The positivity for these primers was 49/50 (98%) of the dogs that presented amplification in at least one of the analyzed samples. The observed result proves that the species prevalent in the city of Montes claros is L. infantum, since it is the species associated with the transmission of CVL in urban areas [19].…”

Section: Discussionsupporting

confidence: 58%

“…According to Dourado (2007) [7] and Silveira et al (2018) [19] PCR results can be influenced by parasite load, used methodology to DNA purification, primer selection, sample type, and presence of inhibitors. For this reason the difference in sensitivity of each methodology studied.…”

Section: Discussionmentioning

confidence: 99%

Molecular Methodology for the Detection of the Leishmania Genus in Different Biological Samples Extracted from Dogs

Sá

Olimpo

Royo

et al. 2019

Acta Scientiae. Vet.

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Background:The leishmaniases are a group of parasitic diseases caused by trypanosomatids belonging to the genus Leishmania, members of the class Kinetoplastida, order Trypanosomatidae, family Trypanosomatidae. Despite innumerous wild species that are infected, the domestic dog is a potential reservoir of Leishmania infantum in urban areas, which expands the transmission pathway to humans. When infected, the dog becomes Visceral Canine Leishmaniasis (CVL), which is characterized by a diverse clinical picture that ranges from asymptomatic to non-specific signs, such as skin lesions, lymphadenomegaly, weight loss, splenomegaly, and/or ocular lesions, thus impairing accurate and rapid diagnosis. In Brazil, it is considered a public health problem since it is endemic in certain regions. Therefore, parasitological, serological, and molecular methods can be used for the detection of the disease. However, the possibility of serological cross-reaction and the occurrence of co-infection with other trypanosomatids decreases the specificity rate to below 100%, which suggests the use of more accurate diagnostic tools. Several molecular targets and starting samples for leishmaniasis diagnosis are already standardized, but there is lack of data allowing the evaluation of the target, as well as which biological material is more efficient for the molecular diagnosis of CVL. The sensitivity of PCR may vary with DNA quality, primer type, parasitemia level, and number of target copies per cell. The spleen, blood, liver, aspirate of bone marrow and lymph nodes are the most frequently used for molecular diagnosis of CVL. The present study aimed to evaluate and compare three protocols of the polymerase chain reaction (PCR) for the molecular diagnosis of CVL in different biological samples removed from animals with different clinical and anatomopathological signs. Materials, Methods & Results:Fifty seropositive dogs for CVL were used, their clinical and anatomopathological signs were evaluated, and were classified as asymptomatic, oligosymptomatic, and polysymptomatic. The PCR was conducted with specific primers for each region of the smaller subunit of rRNA, ITS-1, and KDNA genes. The four types of biological samples (spleen, blood, lymph nodes, and liver) were analyzed. Among the 50 dogs studied, 19 (38%) were in the asymptomatic group; all the animals showed healthy appearance, 15/50 (30%) of the dogs presented up to two symptoms, being classified as oligosymptomatic. The polysymptomatic dogs [16/50 (32%)] presented with a compromised clinical status. The study showed that all dogs had a positive result in at least one analyzed PCR methodology. Comparing the results of the three types of tests in the four types of studied samples, the nested-PCR was the one which presented a greater frequency of amplifications. Of a total of 200 samples, 124 showed amplification, showing a 62% sensitivity. Discussion: The results revealed that, when 50 dogs were analyzed, the spleen tissue samples had a better performance in all the tests, but the se...

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“…e Leishmania spp. são descritas associadas em cães com erliquiose com bastante frequência na literatura, sendo considerado um fator de prognóstico (p = 0,0030), em que as coinfecções podem potencializar a patogênese da erliquiose canina e os sinais clínicos se tornarem mais intensos (SILVEIRA et al, 2018;SOARES et al, 2018;De TOMMASI et al, 2013). No presente trabalho foi observado 25,54% (35/137) (SHIPOV et al, 2008;HARRUS et al, 1997).…”

Section: Resultsunclassified

Prevalência E Fatores De Risco Associados À Infecção Pelo Vírus Da Artrite Encefalite Caprina Em Caprinos No Estado Do Maranhão

Costa¹,

Melo²,

Silva³

et al. 2020

A Pesquisa Nos Diferentes Campos Da Medicina Veterinária 3

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PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil

Cited by 12 publications

References 13 publications

Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum

Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum

Molecular Methodology for the Detection of the Leishmania Genus in Different Biological Samples Extracted from Dogs

Prevalência E Fatores De Risco Associados À Infecção Pelo Vírus Da Artrite Encefalite Caprina Em Caprinos No Estado Do Maranhão

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