PCR-SSCP of growth hormone gene and its association with body weight in Black Bengal goat

Abstract

Present study was undertaken to investigate the single nucleotide polymorphism within growth hormone gene and its correlation with body weight in Black Bengal goat. Two fragments of growth hormone gene, 245 bp fragment (partial intron 1, exon 2 and partial intron 2) and 472 bp fragment (partial intron 2, exon 3 and intron 3 and partial exon 4) were analyzed for detection of polymorphism expected to be present at this locus. SSCP of 245 bp and 472 bp fragment revealed 4 and 5 genotypes, respectively. Sequencing revealed substitution at 5 places in 245 bp fragment whereas at 6 places in 472 bp fragment of growth hormone gene. Least square analysis revealed that only 472 bp fragment genotypes had significant effect on body weight at 6 and 9 month of age. Animals having AC genotype had the highest birth weight, whereas animals having CC genotype had lowest birth weight.

Single-Nucleotide Polymorphisms of Growth Hormone Gene and Its Relationship with Growth Traits in Black Bengal Goats

“…The substitution of a guanine (G) at position 781 causes an amino acid change from serine to glycine, while the A1575G does not result in a change in amino acid [ 13 ]. The SNPs of the GH gene in BB goats have been studied using different techniques, such as SSCP techniques, and an association with birth weight has been demonstrated [ 18 , 19 , 20 ].…”

“…Moreover, a study on the 245 bp fragments (partial intron 1, exon 2, and partial intron 2) of the GH gene polymorphisms in BB goats revealed five genotypes (AA, AB, AC, AD, and CC), and those with the AC genotype had greater birth weight compared to those with the CC genotype [19]. Another study, specifically that of Dayal (2016) [20], showed that the 472 bp fragment genotypes (partial intron 2, exon 3, intron 3, and partial exon 4), but not the 245 bp fragment genotype, had a significant effect on body weight at 6 and 9 months of age in BB goats. However, the associations between A781G or A1575G of the GH gene and the growth traits in BB goats have not been explored yet.…”