PCSK9-related LDL-C value is correlated to Rab5 isoform expression level

Naseri, Faezeh; Mohammadi, Asghar; Hosseni, Bita; Shabani, Mohammad; Piran, Sadegh; Soltanmohammadi, Elham; Najafi, Mohammad

doi:10.1016/j.genrep.2016.12.012

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…To further test the feasibility of the proposed immunosensor, 10 human serum samples (from University-Town Hospital of Chongqing Medical University, China) were used for PCSK9 analysis, including serum samples from eight patients with high LDL values and serum samples from two normal subjects. As shown in Table S2, consistent with literature reports, the PCSK9 concentration is positively correlated with the LDL value. Moreover, the test results of the ECL immunosensor are also consistent with those of enzyme-linked immunosorbent assay, and the relative errors ranged from 1.94 to 7.99%, indicating the potential feasibility of the immunosensor for real sample detection.…”

Section: Resultssupporting

confidence: 91%

“…The data are shown in Table 1; the recovery response fluctuates from 94 to 102.4%, with a variation of RSD ranging from 5.26 to 6.37%, which indicates the potential applicability of this sensor in clinical analysis. S2, consistent with literature reports, 57 the PCSK9 concentration is positively correlated with the LDL value. Moreover, the test results of the ECL immunosensor are also consistent with those of enzyme-linked immunosorbent assay, and the relative errors ranged from 1.94 to 7.99%, indicating the potential feasibility of the immunosensor for real sample detection.…”

Section: ■ Experimental Sectionsupporting

confidence: 92%

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Novel Ce(III)-Metal Organic Framework with a Luminescent Property To Fabricate an Electrochemiluminescence Immunosensor

Zhou

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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We designed a novel luminescent metal–organic framework (MOF) named Ce-TCPP-LMOF (CTM) through a simple one-pot solvothermal method. CTM was synthesized by using the emerging electrochemiluminescent (ECL) material (4,4′,4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) as the organic ligand and Ce(III) as the metal node. We found that CTM not only has the remarkable ability to emit light but also has a uniform “sandwich biscuit” shape and suitable nanoscale size, which are promising for further applications. We also applied CTM to construct a novel ECL immunosensor and achieve sensitive detection of the proprotein convertase subtilisin/kexin type 9 (PCSK9), a biomarker related to cardiovascular diseases. To further amplify the ECL signal of CTM, a novel dual-amplified signal strategy was established by inducing a polyamidoamine dendrimer (PAMAM) and gold nanoparticles (AuNPs). Importantly, we first proved that the ECL signal of the CTM/S2O8 2– system could be enhanced by the PAMAM electric field. As the electron transfer rate was accelerated by the AuNP layer, this ECL signal was further enhanced in AuNP-modified electrodes. The ECL immunosensor showed desirable performance for PCSK9 analysis within a detection range of 50 fg mL–1 to 10 ng mL–1 and a low limit of detection of 19.12 ± 2.69 fg mL–1. Real sample detection suggested that the immunosensor holds great potential for analyzing clinical serum samples.

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Section: Resultssupporting

confidence: 91%

Section: ■ Experimental Sectionsupporting

confidence: 92%

Novel Ce(III)-Metal Organic Framework with a Luminescent Property To Fabricate an Electrochemiluminescence Immunosensor

Zhou

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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Circulating low density lipoprotein (LDL)

Khosravi

Hosseini‐Fard

Najafi

2018

Hormone Molecular Biology and Clinical Investigation

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Low-density lipoprotein (LDL) particles are known as atherogenic agents in coronary artery diseases. They modify to other electronegative forms and may be the subject for improvement of inflammatory events in vessel subendothelial spaces. The circulating LDL value is associated with the plasma PCSK-9 level. They internalize into macrophages using the lysosomal receptor-mediated pathways. LDL uptake is related to the membrane scavenger receptors, modifications of lipid and protein components of LDL particles, vesicular maturation and lipid stores of cells. Furthermore, LDL vesicular trafficking is involved with the function of some proteins such as Rab and Lamp families. These proteins also help in the transportation of free cholesterol from lysosome into the cytosol. The aggregation of lipids in the cytosol is a starting point for the formation of foam cells so that they may participate in the primary core of atherosclerosis plaques. The effects of macrophage subclasses are different in the formation and remodeling of plaques. This review is focused on the cellular and molecular events involved in cholesterol homeostasis.

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Association Between Fat-soluble Vitamins and Lipid Profile in the Overweight Population

Piran

Sarmasti

Shabani

et al. 2020

FNA

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Background &Aims: It is well-known that the coronary artery stenosis is related to lipid profile. This is a descriptive cross-sectional study to investigate the relationship between the serum fat-soluble vitamins (A, E and D), circulating proprotein convertase subtilisin/kexin type 9 (PCSK9), and lipid profile in the study population. Methods: A total of 120 overweight subjects were participated in this study. The circulating PCSK9 and vitamin D were measured by ELISA technique. The serum vitamin A and vitamin E amounts were simultaneously measured by the HPLC method. The Serum Small Dense LDLCholesterol (sdLDL-C) values were evaluated using heparin-Mg2+ precipitation technique. The lipid profile was measured by routine laboratory techniques. Results: The serum vitamin E values correlated significantly to vitamin A (r= 0.47, P= 0.0001), VLDL-C (r= 0.30, P= 0.002), total cholesterol (r= 0.309, P= 0.001), PCSK9 (r= 0.233, P= 0.01) and total triglyceride (r= 0.61, P= 0.0001) values. The circulating PCSK9 values correlated significantly to LDL-C (r= 0.17, P= 0.05) and total cholesterol (r= 0.23, P= 0.009) values. However, there were not correlations between the levels of serum D and A vitamins, the serum LDL-C, sdLDL-C and total cholesterol values. Conclusion: The data showed the correlations between serum vitamin E and PCSK9-related LDLC values lower than the normal range. Furthermore, the results suggested a nutritional need on the patents considering supplementation or fortification of vitamin E for the overweight subjects with higher LDL-C levels.

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PCSK9-related LDL-C value is correlated to Rab5 isoform expression level

Cited by 3 publications

References 19 publications

Novel Ce(III)-Metal Organic Framework with a Luminescent Property To Fabricate an Electrochemiluminescence Immunosensor

Novel Ce(III)-Metal Organic Framework with a Luminescent Property To Fabricate an Electrochemiluminescence Immunosensor

Circulating low density lipoprotein (LDL)

Association Between Fat-soluble Vitamins and Lipid Profile in the Overweight Population

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