Pd23-10 real-Time Mri Reveals a Change in Prostatic Urethral Angle During Voiding

Abstract

qRT-PCR and Western blot analysis. Functional and morphological changes in mitochondria were examined. In vivo, forty SD rats (6 wks of age) were divided into four groups and bred until twenty weeks: control (CON), High Fat Diet (HFD), HFDþ300 mg/kg/d NAC (HFDþLNAC), and HFDþ900 mg/kg/d NAC (HFDþHNAC)}. At the end of the 20 weeks, blood and bladder smooth muscle samples were collected, and OS and contractile levels were examined.RESULTS: In vitro, high glucose significantly enhanced oxidative stress, inflammation and fibrosis in BSMCs. Moreover, the increase in cytokine expression was accompanied by enhanced NLRP3 expression and the phosphorylation of NF-kB. However, N-acetylcysteine had an inhibitory effect on the expression of ROS/NLRP3/NF-kB and the dissolution of mitochondria in HG-treated BSMCs. In vivo, compared to those in the control group, the mean contractility and frequency of bladder smooth muscles in the HFD group was significantly decreased (p<0.01). The mean contractility in the LNACþHFD group was significantly higher than that in the HFD group (p<0.01). Interestingly, the HNACþHFD group did not show any recovery in muscle contractility but exhibited significantly reduced bladder contractile frequency (p<0.01).CONCLUSIONS: Our findings indicated that high glucoseinduced oxidative stress, inflammation and fibrosis are mainly mediated by the ROS/NLRP3/NF-kB signaling pathways and mitochondrial changes in BSMCs. NAC ameliorated these changes by blocking the ROS/NLRP3/NF-kB signaling pathways and protecting mitochondrial function and morphology.