Pdcd4 protein and mRNA level alterations do not correlate in human lung tumors

In vivo study on the effects of microcystin extracts on the expression profiles of proto-oncogenes (c-fos, c-jun and c-myc) in liver, kidney and testis of male Wistar rats injected i.v. with toxins

“…As for other kinds of genes, similar phenomenon also existed. In squamous cell carcinoma samples, in contrast to the suppression of Pdcd4 mRNA, Pdcd4 protein level remained unchanged or even testis c-myc up-regulated (Kalinichenko et al, 2008). Bergmann et al (2008) demonstrated that Ferroportin and DMT1 mRNA levels were significantly higher in cirrhotic human livers than in controls, whereas the levels of proteins were lower in cirrhotic livers than in controls.…”

Loss of programmed cell death 4 induces apoptosis by promoting the translation of procaspase-3 mRNA

“…The difference between changes in the Pdcd4 mRNA and protein expression during apoptosis is consistent with the previous reports suggesting that changes in the mRNA levels are not always paralleled by concomitant changes in the protein levels. 17,27 This observation may be explained by our data implicating miR-199a-5p-operated translational repression in the apoptotic downregulation of the Pdcd4 protein expression independent of the mRNA expression: even though Pdcd4 mRNA is continuously expressed, its translation is inhibited by action of miR-199a-5p, resulting in the decrease of the protein. In addition, this observation may be supported by the previous report describing the involvement of an RNA-binding protein in the Pdcd4 mRNA stability and translation.…”

Protein kinase GSK3β regulates tumor suppressor pdcd4 expression in lung cancer cells

“…On the next day after seeding, the cells were incubated for 18 h with the following inhibitors: (1) rapamycin, an inhibitor of the mTOR 1 signaling complex (mTORC1) (Calbiochem, United States); (2) LY294002 (Promega, United States), which inhibits phosphoinositide 3 kinases, which are activators of Akt protein kinase; or (3) Akt inhibitor X (Calbio chem, United States), an inhibitor of Akt protein kinase itself. The lysates prepared from the cells treated and not treated with the inhibitors were ana lyzed by immunoblotting with the antibodies against the Pdcd4 protein, which were obtained by us earlier [8]. To control the total amount of protein in lanes, we detected α tubulin using monoclonal antibodies DM1A against α tubulin (Sigma, United States).…”