PDGF Gene Therapy Enhances Expression of VEGF and bFGF Genes

Machens, Hans‐Günther; Niedworok, Christian; Spanholtz, Timo; Maichle, A.

doi:10.1097/01.prs.0000233442.86110.87

Cited by 4 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these, VEGF and b‐FGF are the two key players of wound healing . VEGF has been shown to influence angiogenesis via the HIF‐1α pathway induced by hypoxia , whereas b‐FGF has positive effects on proliferation, migration and angiogenic processes . Earlier studies have already shown that adipose‐derived stem cells (ASC) can positively influence wound healing, as they are attracted to the wound site and influence wound healing processes via paracrine mechanisms as well as fusion and differentiation, for example, into keratinocytes or fibroblasts .…”

Section: Introductionmentioning

confidence: 99%

Acute and chronic wound fluids inversely influence adipose‐derived stem cell function: molecular insights into impaired wound healing

Koenen

Spanholtz

Maegele

et al. 2013

International Wound Journal

View full text Add to dashboard Cite

Wound healing is a complex biological process that requires a well-orchestrated interaction of mediators as well as resident and infiltrating cells. In this context, mesenchymal stem cells play a crucial role as they are attracted to the wound site and influence tissue regeneration by various mechanisms. In chronic wounds, these processes are disturbed. In a comparative approach, adipose-derived stem cells (ASC) were treated with acute and chronic wound fluids (AWF and CWF, respectively). Proliferation and migration were investigated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and transwell migration assay. Gene expression changes were analysed using quantitative real time-polymerase chain reaction. AWF had a significantly stronger chemotactic impact on ASC than CWF (77·5% versus 59·8% migrated cells). While proliferation was stimulated by AWF up to 136·3%, CWF had a negative effect on proliferation over time (80·3%). Expression of b-FGF, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 was strongly induced by CWF compared with a mild induction by AWF. These results give an insight into impaired ASC function in chronic wounds. The detected effect of CWF on proliferation and migration of ASC might be one reason for an insufficient healing process in chronic wounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Acute and chronic wound fluids inversely influence adipose‐derived stem cell function: molecular insights into impaired wound healing

Koenen

Spanholtz

Maegele

et al. 2013

International Wound Journal

View full text Add to dashboard Cite

show abstract

“…[12][13][14][15][16][17][18] On the other hand, growth factors have shown promise in improving flap survival through increased angiogenesis and blood supply to the flap. [19][20][21][22][23][24][25][26][27][28][29] Among the more common factors, vascular endothelial growth factor (VEGF), a 35 to approximately 45 kDa homodimeric glycoprotein, has emerged as the central signaling pathway for many of these growth factors [30][31][32][33] and is thus the most widely investigated and successful of these agents. Nevertheless, the mechanisms of the effects are still not clear.…”

mentioning

confidence: 99%

Effects of Vascular Endothelial Growth Factor on Survival of Surgical Flaps: A Review of Experimental Studies

Fang

Lineaweaver

Chen

et al. 2013

J reconstr Microsurg

View full text Add to dashboard Cite

Partial or complete necrosis of skin flaps remains a significant problem in plastic and reconstructive surgery. Growth factors have shown promise in improving flap survival through increased angiogenesis and blood supply to the flap. Vascular endothelial growth factor (VEGF) is the most widely investigated and successful one. But the mechanisms of the effects are still not very clear. In the course of a series of experiments, we indicated that tissue survival of surgical flaps could be improved by both preoperative (sustained phase effect) and intraoperative (acute phase effect) application of VEGF. We reviewed both experimental and clinical investigations on the use of VEGF with surgical flaps to summarize the evidence of both phases of VEGF activity in promotion of flaps survival in detail. With the combinations of acute and sustained phases of effects, VEGF protein and gene, VEGF morphologic actions, and VEGF histochemical modulations suggest a pattern of VEGF activity that can be superimposed on classic descriptive mechanisms of tissue survival of flaps.

show abstract

“…Enhancing angiogenesis in the scaffold by achieving permanently high concentrations of growth factors still presupposes the presence of large amounts of stem cells. Transfection of cells with gene vectors has been shown feasible for increasing proangiogenic factor release [ 4 , 23 ], but due to ethical problems alternative methods need to also be evaluated. Therefore, cell stimulation by cytokines after seeding in the scaffold might be less critical and even easier to realize.…”

Section: Discussionmentioning

confidence: 99%

LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration

et al. 2015

View full text Add to dashboard Cite

High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.

show abstract

PDGF Gene Therapy Enhances Expression of VEGF and bFGF Genes

Cited by 4 publications

References 1 publication

Acute and chronic wound fluids inversely influence adipose‐derived stem cell function: molecular insights into impaired wound healing

Acute and chronic wound fluids inversely influence adipose‐derived stem cell function: molecular insights into impaired wound healing

Effects of Vascular Endothelial Growth Factor on Survival of Surgical Flaps: A Review of Experimental Studies

LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration

Contact Info

Product

Resources

About