PDMS-b-PMOXA polymersomes for hepatocyte targeting and assessment of toxicity

Biomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironment

“…Possible internalisation mechanisms of various PMOXA-PDMS-PMOXA-based polymersomes and their high cytocompatibility in various cell lines have already been reported 17 , 57 – 59 . Here, we evaluated the cytocompatibility of the biomimetic AOs by testing their cellular toxicity using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay before studying their intracellular activation and enzymatic activity.…”

Biomolecule–polymer hybrid compartments: combining the best of both worlds

“…3). [33][34][35][36][37] Apart from direct visualization by TEM, laser light scattering is one of the routine measurements for simultaneous size and morphology determination of nanosized compartments. For dynamic light scattering (DLS), the intensity fluctuations of the scattered laser light due to the Brownian motion of the vesicles are used to determine the hydrodynamic radius by Stokes-Einstein relation.…”

DNA-directed arrangement of soft synthetic compartments and their behavior in vitro and in vivo

“…12 For polymersome formation, we selected the diblock copolymer poly(dimethylsiloxane)-block-poly(2-methyloxazoline) (PDMS-PMOXA) because of its low toxicity and biocompatibility. 36,37 As previously shown, the combination of PMOXA-PDMS-PMOXA triblock copolymer with azide-functionalized diblock PDMS-PMOXA copolymer, bearing a longer PMOXA block, forms polymersomes with exposed azide functional groups on the polymersomes' surface. 12 We then linked dibenzocyclooctyne (DBCO)-functionalized ssDNA (DBCO-ssDNA) to the polymersomes and promoted their zipping by DNA hybridization.…”