PDMS-free microfluidic cell culture with integrated gas supply through a porous membrane of anodized aluminum oxide

Bunge, Frank; Driesche, Sander van den; Vellekoop, Michael J.

doi:10.1007/s10544-018-0343-z

Cited by 10 publications

(8 citation statements)

References 32 publications

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“…Membranes in microfluidic systems can be fabricated from several materials, including inorganic, organic, or combinations. Bunge et al 130 designed a 3 μm thickness porous membrane made of aluminum oxide for cell culture. The membrane was gas permeable and sandwiched between two closed microfluidic chambers.…”

Section: ■ Devices Formed From Multiple Materialsmentioning

confidence: 99%

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

et al. 2019

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Section: ■ Devices Formed From Multiple Materialsmentioning

confidence: 99%

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

et al. 2019

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“…It is well established that PDMS readily absorbs small hydrophobic molecules, including compounds added to culture medium, cell signaling compounds, and the therapeutics often being assessed in cell–drug interaction studies. − Consequently, the use of PDMS-based devices can result in misrepresentations of drug toxicity and efficacy. The accuracy of the observed drug interactions and PK/PD models of these systems is inherently limited, or, if the PDMS absorption of the moiety of interest is estimated/modeled for, require a significant amount of additional work to be invested. , For microfluidic MPSs, the PDMS channel surfaces can be chemically modified (with materials including polydopamine and polynorepinephrine) to minimize absorption, but such coatings must maintain their stability over the course of experimentation, which may last several weeks. ,,, …”

Section: Introductionmentioning

confidence: 99%

“…PDMS is also slightly autofluorescent, causes Raman scattering, is incompatible with organic solvents, and is gas permeable . Gas permeability is a benefit in certain cases but can lead to evaporation and alterations in medium composition, bubble formation, the inability to culture anoxic cells, and variations in osmolarity. , The oligomers that encompass PDMS also have the potential to leach out, resulting in deformation or potential issues in cell culture. , Notably, the soft-lithography process of many PDMS-based devices requires master molds that take time to develop.…”

Section: Introductionmentioning

confidence: 99%

“…10,11 For microfluidic MPSs, the PDMS channel surfaces can be chemically modified (with materials including polydopamine and polynorepinephrine 16 ) to minimize absorption, but such coatings must maintain their stability over the course of experimentation, which may last several weeks. 10,11,17,18 PDMS is also slightly autofluorescent, 13 causes Raman scattering, 19 is incompatible with organic solvents, 17 and is gas permeable. 17 Gas permeability is a benefit in certain cases but can lead to evaporation and alterations in medium composition, bubble formation, the inability to culture anoxic cells, and variations in osmolarity.…”

Section: ■ Introductionmentioning

confidence: 99%

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Beyond Polydimethylsiloxane: Alternative Materials for Fabrication of Organ-on-a-Chip Devices and Microphysiological Systems

Campbell

Yazbeck

et al. 2020

ACS Biomater. Sci. Eng.

189

175

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Polydimethylsiloxane (PDMS) is the predominant material used for organ-on-a-chip devices and microphysiological systems (MPSs) due to its ease-of-use, elasticity, optical transparency, and inexpensive microfabrication. However, the absorption of small hydrophobic molecules by PDMS and the limited capacity for high-throughput manufacturing of PDMS-laden devices severely limit the application of these systems in personalized medicine, drug discovery, in vitro pharmacokinetic/pharmacodynamic (PK/PD) modeling, and the investigation of cellular responses to drugs. Consequently, the relatively young field of organ-on-a-chip devices and MPSs is gradually beginning to make the transition to alternative, nonabsorptive materials for these crucial applications. This review examines some of the first steps that have been made in the development of organ-on-a-chip devices and MPSs composed of such alternative materials, including elastomers, hydrogels, thermoplastic polymers, and inorganic materials. It also provides an outlook on where PDMS-alternative devices are trending and the obstacles that must be overcome in the development of versatile devices based on alternative materials to PDMS.

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“…This in turn enables testing at an early preclinical stage, where the active ingredient is initially only available in small quantities. Although these microfluidic devices are easily accessible via academic research, the transfer of elaborated standard operation procedures, developed for static cell permeation assays of pharmaceutical drugs, remains challenging [ 7 , 8 , 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Tissue Barrier-on-Chip: A Technology for Reproducible Practice in Drug Testing

et al. 2022

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One key application of organ-on-chip systems is the examination of drug transport and absorption through native cell barriers such the blood–brain barrier. To overcome previous hurdles related to the transferability of existing static cell cultivation protocols and polydimethylsiloxane (PDMS) as the construction material, a chip platform with key innovations for practical use in drug-permeation testing is presented. First, the design allows for the transfer of barrier-forming tissue into the microfluidic system after cells have been seeded on porous polymer or Si3N4 membranes. From this, we can follow highly reproducible models and cultivation protocols established for static drug testing, from coating the membrane to seeding the cells and cell analysis. Second, the perfusion system is a microscopable glass chip with two fluid compartments with transparent embedded electrodes separated by the membrane. The reversible closure in a clamping adapter requires only a very thin PDMS sealing with negligible liquid contact, thereby eliminating well-known disadvantages of PDMS, such as its limited usability in the quantitative measurements of hydrophobic drug molecule concentrations. Equipped with tissue transfer capabilities, perfusion chamber inertness and air bubble trapping, and supplemented with automated fluid control, the presented system is a promising platform for studying established in vitro models of tissue barriers under reproducible microfluidic perfusion conditions.

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PDMS-free microfluidic cell culture with integrated gas supply through a porous membrane of anodized aluminum oxide

Cited by 10 publications

References 32 publications

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

Beyond Polydimethylsiloxane: Alternative Materials for Fabrication of Organ-on-a-Chip Devices and Microphysiological Systems

Tissue Barrier-on-Chip: A Technology for Reproducible Practice in Drug Testing

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