PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

Prauzner-Bechcicki, Szymon; Raczkowska, Joanna; Madej, Ewelina; Pabijan, Joanna; Lukeš, Jaroslav; Šepitka, Josef; Rysz, Jakub; Awsiuk, Kamil; Bernasik, Andrzej; Budkowski, Andrzej; Lekka, Małgorzata

doi:10.1016/j.jmbbm.2014.09.020

Cited by 67 publications

(54 citation statements)

References 55 publications

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“…In addition, PDMS substrates provides a range of mechanical properties that are more physiologically relevant compared with other substrates (Mata et al, ). In this study, PDMS substrates were fabricated with elastic moduli varying from 0.24 to 2.1 MPa, which are in a similar range to those in previous studies (Evans et al, ; Prauzner‐Bechcicki et al, ). Compared to traditional TCPS, our results revealed that elastic moduli of PDMS‐based substrates can more closely simulate the elastic modulus values of periodontal tissues including alveolar bone and cementum.…”

Section: Discussionmentioning

confidence: 94%

Evaluation of polydimethylsiloxane‐based substrates for in vitro culture of human periodontal ligament cells

Yan

Beucken

Yuan

et al. 2019

J Biomedical Materials Res

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Periodontal ligament (PDL) cells are regarded as the cell type with the highest potential for periodontal regeneration. Biophysical cues of the culture substrate are increasingly identified as vital parameters to affect cell behavior. Compared to traditional tissue culture polystyrene (TCPS), polydimethylsiloxane (PDMS) substrates corroborate more closely the elastic modulus values of the physiological environment. Consequently, the aim of this study was to evaluate the effect of PDMS‐based substrates with different stiffness on cellular responses of human PDL cells. PDMS substrates with different stiffness were fabricated by varying the ratio of base to curing component. The influence of PDMS substrates on PDL cell spreading and cytoskeletal morphologies, motility, proliferation, stemness gene expression, and osteogenic differentiation was evaluated and compared to that on conventional TCPS. PDL cells cultured on PDMS substrates exhibited a smaller cell size and more elongated morphology, with less spreading area, fewer focal adhesions, and faster migration than cells on TCPS. Compared to TCPS, PDMS substrates promoted the rapid in vitro expansion of PDL cells without interfering with their self‐renewal ability. In contrast, the osteogenic differentiation ability of PDL cells cultured on PDMS was lower in comparison to cells on TCPS. PDL cells on PDMS exhibited similar cell morphology, motility, proliferation, and self‐renewal gene expression. The stiffer PDMS substrate increased the osteogenic gene expression of PDL cells compared to the soft PDMS group in one donor. These data indicate that PDMS‐based substrates have the potential for the efficient PDL cell expansion.

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Section: Discussionmentioning

confidence: 94%

Evaluation of polydimethylsiloxane‐based substrates for in vitro culture of human periodontal ligament cells

Yan

Beucken

Yuan

et al. 2019

J Biomedical Materials Res

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“…21 Hence, despite the fact that hydrophobic nature of PDMS requests an intermediate protein for cell attachment, 16 in some cases even the usage of PDMS substrate without coating has been suggested to properly investigate effects of bulk stiffness on cell behavior. 21 Additionally, it has been argued that some surface modifications such as plasma treatment can create a thin layer with different elastic behavior from the bulk of substrate which might undermine effect of substrate properties on cell behavior. 29 Here we coated substrates with a very thin layer of fibronectin to allow cell interact with the substrates and sense their stiffness.…”

Section: Discussionmentioning

confidence: 99%

“…17-19 Study of cancerous cells cultured on PDMS substrates with two distinct elastic moduli demonstrated changes in the morphological parameters by substrate rigidity, while other chemical and physical properties of the substrates were kept the same. 21 …”

Section: Introductionmentioning

confidence: 99%

Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness

Goli-Malekabadi¹,

Tafazzoli-Shadpour²,

Tamayol³

et al. 2017

Bioimpacts

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Introduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic conditions. Despite numerous studies, cells by time span sense mechanical properties of the substrate, but the response is not well understood. We hypothesized that time is a major determinant influencing the behavior of cells seeded on substrates of varying stiffness. Methods: We monitored cell spreading, internal structure, 3D topography, and the viability of ECs over 24 hours of culture on polydimethylsiloxane (PDMS) substrates with two different degrees of elastic modulus. Results: Despite significant differences in cell spreading after cell seeding, cells showed a similar shape and internal structure after 24 hours of culture on both soft and stiff substrates. However, 3D topographical images confirmed existence of rich lamellipodia and filopodia around the cells cultured on stiffer PDMS substrates. Conclusion: It was concluded that the response of ECs to the substrate stiffness was time dependent with initial enhanced cellular spreading and viability on stiffer substrates. Results can provide a better comprehension of cell mechanotransduction for tissue engineering applications.

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“…However, coverage with polymer layer changes the mechanical properties of the substrate simultaneously [44–46], especially for hard film on a soft support. This effect can have great consequences for potential applications of polymer coatings in biomedical field as cells are very sensitive to substrate elasticity [28,71–73] and can sense the effective stiffness of rigid objects that are not in direct cellular contact, up to several microns [48]. …”

Section: Resultsmentioning

confidence: 99%

“…Mostly investigated factors influencing cellular response include surface topography [5–12], chemistry [13–18] and wettability [19–21]. However, it has been shown that also mechanical properties of the substrate play an important role for adhesion and proliferation of cells, which can be enhanced or prohibited, depending on subtle changes of substrate elasticity [22–28]. Cells are able to react to external elasticity and even generate internal forces, enabling detection of extracellular environmental properties [29,30].…”

Section: Introductionmentioning

confidence: 99%

Elasticity patterns induced by phase-separation in polymer blend films

et al. 2017

Self Cite

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Systematical studies on the impact of the thickness of thin films composed of polystyrene (PS) or poly(ethylene oxide) (PEO) on the effective elasticity of polymer-decorated soft polydimethylsiloxane substrate were performed. For both investigated polymer films, elasticity parameter was determined from force-displacement curves recorded using atomic force microscopy. Effective stiffness of supported film grows monotonically with film thickness, starting from the value comparable to the elasticity of soft support and reaching plateau for polymer layers thicker than 200 nm. In contrary, for films cast on hard support no significant thickness dependence of elasticity was observed and the value of elasticity parameter was similar to the one of the substrate. Based on these results, non-conventional method to produce elasticity patterns of various shapes and dimensions induced by phase-separation process in symmetric and asymmetric PS:PEO blend films on soft support was demonstrated. Elevated PS domains were characterized by elasticity parameter 2 times higher than lower PEO matrix. In contrary, adhesion force was increased more than 3 times for PEO regions, as compared to PS areas.

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PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

Cited by 67 publications

References 55 publications

Evaluation of polydimethylsiloxane‐based substrates for in vitro culture of human periodontal ligament cells

Evaluation of polydimethylsiloxane‐based substrates for in vitro culture of human periodontal ligament cells

Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness

Elasticity patterns induced by phase-separation in polymer blend films

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