PDMS through-hole fabrication by soft lithography using CH

Visualization of the Cellular Uptake and Trafficking of DNA Origami Nanostructures in Cancer Cells

“…However, Cy5-DNA showed neglectable cellular uptake under our current experimental settings (i.e., 250 nM) as verified both by flow cytometry and confocal microscopy ( Figure 3a,b), in agreement to the findings of Mirkin and collegues. 34,35 Nevertheless, the cellular uptake of Cy5-DNA became prominent at a concentration of 1 μM ( Figure S10), suggesting that a low concentration must be utilized to minimize the interfering effect of Cy5-DNA (e.g., 250 nM in the current study). Cellular uptake experiments were also independently conducted using a noncyanine dye (Alexa-488), and produced similar results ( Figure S11).…”

“…39,40 Mirkin et al reported that scavenger receptors play important roles in the cellular uptake of spherical nucleic acids in multiple mammalian cell lines. 34,40 Biocca et al reported that scavenger receptors are essential for COS fibroblast cells to internalize octahedral DNA nanocages. 41 Therefore, a mechanistic study was performed to assess the involvement of scavenger receptors in the uptake of DONs into H1299 and DMS53 cells.…”

“…Surprisingly, considerable cytotoxicity was observed in H1299 and DMS53 cells treated with the pharmacological inhibitors at the recommended conditions (data not shown). 34,40,43 Inhibitors were diluted significantly in order to maintain apparent cell viability, but no inhibition of cellular uptake of DONs was observed at such conditions ( Figure S18c), possibly due to insufficient blockade of targeting pathways at the reduced concentrations.…”

“…44 Both studies suggested that nanoparticles of high aspect ratio might enter through a similar process: maximizing capturing efficiency via longitudinal alignment, followed by minimizing energy expense via transverse entry. Membrane invaginations appeared flaskshaped, with sizes between 50 and 100 nm, closely resembling caveolae, 34,42 suggesting that LRs may enter H1299 cells through a caveolin-dependent pathway.…”