PDZK1-interacting protein 1(PDZK1IP1) promotes subcutaneous preadipocyte proliferation in goats

Chen, Dingshuang; Wang, Ruilong; Sheng, Xueqing; Zhao, Nan; Lin, Yaqiu; Wang, Yong; Zhu, Jiangjiang; Li, Yanyan

doi:10.1080/10495398.2022.2132952

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…MTT experiments were performed to detect the cells' viability. We planted goat subcutaneous preadipocytes in 96-well plates with a density of 3 × 10 3 per well and cultured them according to the methods of previous articles in our laboratory [28]. Adding 10 µL MTT reagent (solarbio, Beijing, China) into each well of 96-well plates away from light, cells were cultured at 37 • C and 5% CO 2 for 4 h. The absorbance at 490 nm was measured by using an enzyme-labeled instrument.…”

Section: Mtt Assaymentioning

confidence: 99%

Effect of CXCL17 on Subcutaneous Preadipocytes Proliferation in Goats

Fei

et al. 2023

Animals

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The presence or absence of subcutaneous adipose accumulation will affect the energy storage, insulation resistance and metabolism of animals. Proliferation and differentiation of preadipocytes play a significant role in lipid deposition. The objective of this study was to clone the goat CXCL17 gene and investigate its potential functions on goat subcutaneous preadipocytes’ proliferation by gaining or losing function in vitro. The goat CXCL17 gene was cloned by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and bioinformatics analysis was performed. The expression of the CXCL17 gene in the different goat tissues and adipocytes at different differentiation stages was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the cloned sequence of goat CXCL17 gene is 728 bp and the CDS region is 357 bp, encoding 118 amino acids. CXCL17 protein is located in nucleus, cytoplasm, mitochondria and extracellular matrix. Tissue-expression profiles revealed that CXCL17 expressed in all of the examined tissues. In visceral tissues, the highest expression level was found in lung (p < 0.01); in muscle tissues, the highest CXCL17 expression level was found in the longissimus dorsi (p < 0.01) and in adipose tissues, the highest expression level was found in subcutaneous adipose (p <0.01). Compared with those cells before differentiation, CXCL17 expression levels upregulated at 48 h (p < 0.01), 72 h (p < 0.01), 120 h (p < 0.01) and downregulated at 96 h (p < 0.01). Furthermore, the results of crystal violet staining and semi-quantitative assay showed that transfection with 1 μg CXCL17 expression plasmid reduced the cell numbers in vitro. Meanwhile, the expression of CCND1 was significantly decreased. A similar consequence happened after interfering with CXCL17 expression. However, plasmid transfected with 2 μg pEGFPN1-CXCL17 increased the number of cells in vitro. These results suggest that CXCL17 is involved in the proliferation of goat subcutaneous preadipocytes.

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Section: Mtt Assaymentioning

confidence: 99%

Effect of CXCL17 on Subcutaneous Preadipocytes Proliferation in Goats

Fei

et al. 2023

Animals

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“…However, the regulating effects of PDZK1IP1 in regulating lipid deposition in preadipocytes have not been reported. In our previous study, we successfully cloned the goat PDZK1P1 gene sequence and demonstrated that PDZK1IP1 overexpression could promote goat subcutaneous preadipocyte proliferation [ 23 ]. In addition, it is worth noting that bortezomib can induce autophagy in breast cancer cells, while overexpressed PDZK1IP1 can inhibit autophagy in these cells [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

PDZK1-Interacting Protein 1(PDZKIP1) Inhibits Goat Subcutaneous Preadipocyte Differentiation through Promoting Autophagy

Chen

et al. 2023

Animals

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PDZK1IP1 is highly expressed in tumor tissue and has been identified as a tumor biomarker. However, the role of PDZK1IP1 in goat subcutaneous preadipocyte differentiation remains largely unknown. The molecular mechanism of autophagy in regulating the differentiation of goat subcutaneous preadipocytes has not been clarified yet. In our study, PDZK1IP1 gain of function and loss of function were performed to reveal its functions in preadipocyte differentiation and autophagy. Our results showed that the overexpression of PDZK1IP1 inhibited the differentiation of goat subcutaneous preadipocytes, whereas it promoted autophagy. Consistently, the knockdown of PDZK1IP1 demonstrated the opposite tendency. Next, we investigated whether PDZK1IP1 inhibited the differentiation of goat preadipocytes by regulating autophagy. We found that inhibiting autophagy can rescue the PDZK1IP1-induced differentiation restraint in goat subcutaneous preadipocytes. In conclusion, PDZK1IP1 acts as a regulator of adipogenesis, and inhibits goat subcutaneous preadipocyte differentiation through promoting autophagy. Our results will contribute to further understanding the role and mechanism of PDZK1IP1 in controlling adipogenesis.

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PDZK1-interacting protein 1(PDZK1IP1) promotes subcutaneous preadipocyte proliferation in goats

Cited by 2 publications

References 38 publications

Effect of CXCL17 on Subcutaneous Preadipocytes Proliferation in Goats

Effect of CXCL17 on Subcutaneous Preadipocytes Proliferation in Goats

PDZK1-Interacting Protein 1(PDZKIP1) Inhibits Goat Subcutaneous Preadipocyte Differentiation through Promoting Autophagy

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