Pea (Pisum sativum L.)

Griga, Miroslav; Novák, František

doi:10.1007/978-3-642-74448-8_4

Cited by 9 publications

(3 citation statements)

References 65 publications

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“…macrocarpon Ser.) is grown worldwide, extensively in northern USA, Europe, Canada, Russia, and China [1,2]. Due to the delicious flavor and abundant nutrients, its tender shoot, seed, and pod are consumed as vegetables, especially the tender pod [3].…”

Section: Introductionmentioning

confidence: 99%

Comparison of storage and lignin accumulation characteristics between two types of snow pea

Wang²,

Qu³

et al. 2022

PLoS ONE

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Snow pea is a very important vegetable, and its postharvest storage characteristics vary with species. Few studies on the differences in its storage characteristics are available. In this study, postharvest changes in metabolic rate (respiration rate and water loss rate), membrane permeability (relative conductivity), nutrient contents (total sugar, amino acids, starch), lignin, cellulose, β-Glucosidase (β-GC) enzyme activity, texture properties, PG enzyme activity and their relationship were analyzed in large sweet broad peas and small snow peas. On the 8th day of storage, we found that the respiration rate and water loss rate were increased, total sugars and total amino acids were decreased significantly in these two legume vegetables, and that metabolic rate was slower with less nutrients consumed in large sweet broad peas than in small snow peas. Throughout the 8-day whole storage, the lignin and cellulose contents were always lower in large sweet broad peas than in small snow peas. With the increasing storage time, small snow peas were more susceptible to lignification and fibrosis, which was observed in their texture properties. The enzyme activities related to cellulose and pectin degradation (β-GC, PG) also showed the same trend during the storage. At the late stage of storage, the taste of large sweet broad peas was better than that of small snow peas. In conclusion, the storage period of large sweet broad peas was longer than that of the small snow peas, and its lignification degree was lower than that of the small snow peas. Meanwhile, senescence and lignin accumulation led to hardening of snow pea during postharvest storage. Our findings provide a theoretical reference for improving the postharvest storage quality of snow pea and extending the shelf life.

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Section: Introductionmentioning

confidence: 99%

Comparison of storage and lignin accumulation characteristics between two types of snow pea

Wang²,

Qu³

et al. 2022

PLoS ONE

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“…Pea (Pisum sativum L) is a member of the cultivated legume crops belonging to the order Fabales, family Fabaceae and tribe Viciae (Griga and Novak 1990).…”

Section: Introductionmentioning

confidence: 99%

Differentiate of Ten Pea Cultivars ( Pisum Sativum L. ) by RAPD Markers and Seed Storage Proteins = التفريق بين عشرة أصناف من البازيلاء ( Pisum Sativum L. ) بواسطة تقنية RAPD و بروتينات البذور الخاصة بالتخزين

Tahir¹,

Kareem²,

Amin³

2015

JJAS

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The present study involved characterization of variability of random amplified polymorphic DNA (RAPD) markers and protein patterns in some Pisum sativum cultivars. Twenty RAPD primers were used to estimate the genetic diversity in ten pea cultivars. A total of 89 bands were scored, of which 53 bands (59.55%) were polymorphic and two of polymorphic band were unique bands. The average of amplified and polymorphic bands was 4.45 and 2.65, respectively. The highest polymorphic bands found at locus C10, R11 and V03 (5 bands), whereas the lowest number of polymorphic bands found at locus OPA-09, OPA13, OPA-07, P19, N7 and N20 (1 band). Distance matrix based on Jaccard coefficient ranged from 0.333 to 0.774. The highest value was found between O-P622 and O-P234, while the lowest genetic distance was observed between O-P923 and O-P888. A dendrogram revealed two major clusters: cluster I and cluster II. The results of dendrogram indicated that the groups of cluster II is most genetically distant from groups of cluster I. The SDS-PAGE patterns of seed storage proteins, showed that among 23 electrophoretic protein bands, only five bands were recorded to be polymorphic. The distance matrix values ranged from 0.00 to 0.75. At distance 23, cluster analysis sorting the cultivars into two major groups. The dendrogram constructed from both methods divided pea cultivars into two clusters. In this study, analysis using RAPD markers provide more information about genetic diversity among pea cultivars and the results of SDS-PAGE show low variation among pea cultivars.

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“…The in vitro regeneration is the prerequisite step of genetic transformation methods more and more often employed as a component of contemporary breeding programs. The following methods of in vitro cultures were applied to regenerate peas: direct regeneration via meristem cultures, organogenesis, somatic embryogenesis and protoplast cultures (for review: Davies and Mullineaux 1993;Griga and Novak 1990). To obtain transgenic peas, mainly organogenesis de novo (Grant et al 1995;Polowick et al 2000;Puonti-Kaerlas, 1990;Schroeder et al 1993), but also meristem cultures (Bean et al 1997;Davies et al 1993;Nadolska-Orczyk and Orczyk 1999) have been successfully used.…”

Section: Introductionmentioning

confidence: 99%

Organogenesis and long-term micropropagatlon of Polish pea cultivars

et al. 2011

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The complete protocol for regeneration and long-term micropropagation of several Polish cultivars of pea (Pisum sativum L.) has been elaborated. The shoots were the most likely regenerated via de novo organogenesis. The adventitious buds formed in callus derived from cotyledons tissue adjacent to the axillary meristems of immature embryos. All cultivars calli regenerated several shoots per explant on the MS medium supplemented with B5 vitamins and 4.5 mgl -1 of BAP, however some differences in regeneration capacity among cultivars were observed. The plantlets were subsequently micropropagated with slightly higher efficiency and preserving a good viability over the long-term culture on a medium containing 2.0 mgl -1 than one with 4.5 mgl -1 of BAP. The additional step of the pre-conditioning culture of multiplicated shoots on a medium with very low BAP concentration i.e. 0.02 mgl -1 was applied and appeared to be beneficial before rooting in vitro or grafting. The modified MS-derived medium with the half-strength of MS macroelements but with the full original dose of calcium and supplemented with B5 vitamins and 1.0 mgl -1 of NAA was developed for effective rooting. The shoots were also sufficiently transferred into ex vitro conditions using grafting. The majority of the regenerated plants had adapted to in vivo conditions in a greenhouse and subsequently has set seeds. The presented protocol provides relatively efficient rate of de novo pea regeneration and would be useful for Agrobacterium-mediated transformation purposes.

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Pea (Pisum sativum L.)

Cited by 9 publications

References 65 publications

Comparison of storage and lignin accumulation characteristics between two types of snow pea

Comparison of storage and lignin accumulation characteristics between two types of snow pea

Differentiate of Ten Pea Cultivars ( Pisum Sativum L. ) by RAPD Markers and Seed Storage Proteins = التفريق بين عشرة أصناف من البازيلاء ( Pisum Sativum L. ) بواسطة تقنية RAPD و بروتينات البذور الخاصة بالتخزين

Organogenesis and long-term micropropagatlon of Polish pea cultivars

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