Peach and Cherry Agroindustrial Wastes: New and Economic Sources for the Production of Lignocellulolytic Enzymes

Abstract

Pleurotus eryngii was incubated on both peach and cherry agroindustrial wastes by solid state fermentation for 30 days without/with pretreatment conditions. The lignocellulosic substrates were pretreated with dilute acid and alkaline solutions, hot water before incubation. The maximum carboxymethyl cellulase and xylanase activities peaked on 3 rd and 5 th days under control conditions of both wastes, respectively. The highest laccase and manganese peroxidase activities reached to their maximum on 17 th day as 2193.06 ± 50.4 UL -1 and 732.73 ± 19.8 UL -1 , respectively. The highest aryl alcohol oxidase activity was obtained as 239.25 ± 7.3 UL -1 in control condition of peach cultures. The used pretreatment methods had generally negative effects on lignocellulolytic enzyme production. The highest lignocellulolytic activities were detected using peach wastes. To results, these wastes could be used as alternative, new and economic energy sources to produce high amounts of lignocellulolytic enzymes.

Decolorization and degradation potential of enhanced lignocellulolytic enzymes production by Pleurotus eryngii using cherry waste from industry

“…Various agroindustrial wastes were investigated in the production process of lignocellulolytic enzymes with SSF . According to the literature published so far, CW in the production of enzymes used in various industries, including lignocellulolytics by fungi, has not been reported except for the other research of Akpinar and Ozturk Urek . Thus, this waste is a new natural substrate for use in the productions of lignocelluloytic enzymes by P. eryngii under SSF conditions.…”

“…P. eryngii (DC.) Gillet (MCC58) provided from the Agroma Mushroom culture collection (Denizli, Turkey) is preferred because it produces high levels of lignocellulolytic enzymes in SSF . P. eryngii was grown on (g L −1 ) malt extract, 30.0; peptone; 3.0; and agar, 15.0 (pH 5.6) at 25 ± 0.5 °C .…”

“…After fermentation, production medium was suspended in 50 mL acetate buffer (50 mM, pH 5.0) after each flask had been removed and shaken for 1 hour (h) at 180 rpm. Then, it was centrifuged (4 ± 1 °C, 15 120 g, 10 min) , and obtained supernatants were utilized for analysis of the lignocellulolytic enzymes activities, at the same time, the changes in the protein levels has studied throughout the cultivation period. Experiments were performed triplicate and samples were analyzed in triplicate.…”

“…One of the fermentation methods used in the production of lignocellulolytic enzymes, solid‐state fermentation (SSF), is the process that best imitates the common habitat of most filamentous fungi . In SSF process, the solid substrate is an insoluble matter functioning both as physical support and source of nutrients.…”

“…Also, it is the new substrate material for the productions of lignocellulolytic enzymes under SSF conditions. Some culture conditions such as presence/absence of micronutrients are believed to have an important effect on lignocellulolytic enzymes productions by fungi . In this study, the effects of different inducers such as copper, iron, Tween 80, ammonium nitrate, and manganese were investigated in order to increase the productions of lignocellulolytic enzymes by P. eryngii .…”