The anaerobic ability (AnA) is given by the sum of the alactic and lactic (AlA) ability. The cycloergonometer has been used at the maximal 30-second strength (max 30 ) to the AnA approach (mean power and fatigue index). Presently, the AnA is not directly measured, and it is required an operational approaching, such as the measurement of the performance and the blood lactate concentration ([La]). In high-level velocity runners, it is expected that on long-endurance, that is, 60 seconds (max 30 ) strength there would be a higher AnA demand, and aiming the ecological validity, it must be applied on the track. The purpose of this study was to make a comparison between the derived variables of the (max 30 ) and (max 60 ) in runners. Eight male national and international 19-27 years old athletes were submitted to max 30 and max 60 running tests on an official synthetic track, where it was performed an arterialized blood collection from the ear lobule immediately 1, 3, 5, 7.5, and 10 minutes after the strength. To make a comparison between the max 30 and the max 60 , it was used the t-Student test and the simple Pearson's correlation to check the association between variables. The lactate concentrations were significantly higher at max 60 than at max 30 (20.9 ± 1.2 vs. 18.2 ± 1.9 mM, p < 0.05). Oppose to this, the mean velocities were significantly lower (7.9 ± 0.2 vs. 9.1 ± 0.2 m.s -1 , respectively). Significant correlations were found between max 30 and max 60 (r = 0.92; p < 0.05). However, it was not seen the same as to the lactate (r = 0.62; p > 0.05). According to these athletes' characteristics, who are highly tolerant to elevated [La], the derived test variables with duration/distance close to the 60 s/500 m seem to be more adequate as AnA indexes.