Peak-fitting assisted SERS strategy for accurate discrimination of carboxylic acid enantiomers

Tao, Jing; Li, Dan; Guo, Yanyan; Deng, Wei

doi:10.1039/d1cc04506g

Cited by 14 publications

(7 citation statements)

References 16 publications

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“…For estimating, N SERS the following assumptions are taken into consideration (i) the reporter molecule, 4-MPBA, is present as a monolayer over the plasmonic substrate, (ii) 4-MPBA adopts a tilted configuration on silver surfaces, and (iii) the topological polar surface area of MPBA molecule is 0.34 nm 2 . [40,41] Therefore, N SERS can be expressed as equation ( 5)…”

Section: Sers Substrate Preparation and Characterizationmentioning

confidence: 99%

A Practical Nanoplasmonic SERS Substrate for Differential Diagnosis of Lung Normal and Cancer Cells through Multivariate Statistical Analysis

Abraham,

Emmanuel,

Ajikumar

et al. 2023

ChemNanoMat

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Lung cancer ranks first for cancer‐related mortalities primarily due to late diagnosis. Though Surface‐Enhanced Raman spectroscopy (SERS) is a popular bioanalytical technique, its direct application to diagnosis is impeded by low data reproducibility. Colloidal nanoparticles suffer from SERS intensity fluctuations due to unavoidable aggregation, and Brownian and diffusion motions in biological samples. The processes for solid‐state SERS substrates are either sophisticated or difficult to reproduce. Herein, we revisit the well‐established thermal evaporation process for the easy and reproducible preparation of silver nanoparticles loaded SERS glass substrates. The static mode of thermal evaporation yielded closely packed and uniformly distributed silver nanoparticles. The properties of these nanoparticles are tuned for the best performance by controlling the thermal evaporation process. And SERS substrate exhibited a reasonably good enhancement factor of ~ 105 with uniformity and reproducibility < 6 % RSD over a large area. It was utilized for label‐free SERS fingerprinting of lung adenocarcinoma cells A549 and normal lung fibroblast cells, WI38. The obtained data shows a slight distinction of Raman fingerprints in terms of certain biomolecules like nucleic acids, proteins, and lipids. Further multivariate statistical tools have been utilized which ensures a clear divergence between the cancerous cells and normal cells.

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Section: Sers Substrate Preparation and Characterizationmentioning

confidence: 99%

A Practical Nanoplasmonic SERS Substrate for Differential Diagnosis of Lung Normal and Cancer Cells through Multivariate Statistical Analysis

Abraham,

Emmanuel,

Ajikumar

et al. 2023

ChemNanoMat

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“…Normally, it is very difficult to directly distinguish enantiomers by using the SERS method because of their similar Raman-active vibration signals from l -form and d -form enantiomers. To address the issue above, a strategy to modify SERS substrates with chiral or achiral molecules has been proposed for discriminating enantiomers via enantiodifferential interaction. − Unfortunately, such an SERS assay suffers from low enantioselectivity because the signal differences between a pair of enantiomers are not remarkable. Therefore, it is urgently desired to explore a novel chiral selector to improve the SERS enantioselective detection.…”

Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Enantioselective Detection of Gastric Cancer-Related d-Amino Acids in Saliva Based on Enzyme-Mediated Cascade Reaction

Liu,

Zhang,

Zheng

et al. 2023

Anal. Chem.

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The unusual D-amino acids (D-AAs), as the counter enantiomer of usual L-amino acids (L-AAs), have evoked increasing attention because of their potential relevance with diseases. Accordingly, it is essential to establish sensitive and selective detection methods for D-AAs without the interferences from L-AAs. The surface-enhanced Raman scattering (SERS) technique is efficacious for the detection of molecules but routinely ineffective in enantiomeric differentiation. D-Proline (D-Pro) and D-alanine (D-Ala) are regarded as biomarkers of gastric cancer. Herein, Ramanactive boronate modified SERS chips are constructed to develop a Damino acid oxidase (DAAO)-mediated cascade reaction-based SERS enantioselective assay for D-Pro and D-Ala. The principle is that DAAO selectively catalyzes the deamination of D-Pro and D-Ala, and the produced H 2 O 2 oxidizes boronate to present a new SERS peak at 883 cm −1 for quantitative analysis in a ratiometric way. A linear range from 20 to 400 μmol/L and a limit of detection down to 14.8 μmol/L are reached. In addition, interferences from L-AAs and many other possible species coexisting in biofluids with the detection of D-Pro and D-Ala are ignorable. Enzyme-mediated cascade reaction-based SERS chips are further utilized for saliva sample analysis, and the total levels of D-Pro and D-Ala in salivary samples from gastric cancer patients are much higher than those of healthy persons. This work provides a solution for SERS enantioselective analysis and noninvasive screening chiral biomolecules for disease diagnosis.

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“…Instead, designed molecules are used as chirality selectors/sensors to interact differently with enantiomers, and such differential molecular interactions are converted into enantiomer-specific optical signals collected by various spectroscopies . With this view in mind, surface-enhanced Raman scattering (SERS) spectroscopy, due to its superior sensitivity to bond vibration changes induced by molecular interactions, could be a potential and good alternative for enantiomeric discrimination. , However, so far, the application of SERS in chiral detection is still in its infancy. − …”

Section: Introductionmentioning

confidence: 99%

Chiral Detection of Glucose: An Amino Acid-Assisted Surface-Enhanced Raman Scattering Strategy Showing Opposite Enantiomeric Effects on SERS Signals

et al. 2022

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It is of considerable concern to establish chiral detection methods for revealing enantioselective interactions among chiral molecules. Surfaceenhanced Raman scattering (SERS) spectroscopy is sensitive to molecular interaction due to bond variations. However, its application in chiral detection is underexplored. Inspired by the chiral selectivity toward glucose and amino acids in life, we herein propose a SERS strategy based on molecular interactions for the discrimination of D-and L-glucose (Glu) using chiral phenylalanine (Phe) decorated on gold nanoparticles as a chirality selector and Raman reporter. Interestingly, the SERS signal of L-Phe is enhanced by D-Glu but suppressed by L-Glu. In contrast, the SERS signal of D-Phe is increased by L-Glu but decreased by D-Glu. According to the above-observed intensity change (ΔI) of the SERS signal of Phe induced by Glu, it is easy to determine the chiral configurations (judged by the positive or negative sign of ΔI), enantiomeric excess (ee) values, and concentrations (estimated by the magnitude of ΔI) of Glu. Taking advantage of the high SERS enhancement and opposite enantiomeric effects on SERS signals, the proposed strategy enables enantiomeric discrimination at a low Glu concentration (10 −6 mol/L) and is further exerted for the noninvasive detection of D-/L-Glu in saliva samples. In contrast, the common chiroptical analysis tool of circular dichroism (CD) spectroscopy failed to directly detect Glu enantiomers.

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Peak-fitting assisted SERS strategy for accurate discrimination of carboxylic acid enantiomers

Abstract: A novel peak-fitting assisted SERS (PF-SERS) strategy was developed for the first time to discriminate the carboxylic acid enantiomers. The PF-SERS method offered substantial improvement in accuracy, quantitative performance of...

Cited by 14 publications

References 16 publications

A Practical Nanoplasmonic SERS Substrate for Differential Diagnosis of Lung Normal and Cancer Cells through Multivariate Statistical Analysis

A Practical Nanoplasmonic SERS Substrate for Differential Diagnosis of Lung Normal and Cancer Cells through Multivariate Statistical Analysis

Surface-Enhanced Raman Scattering Enantioselective Detection of Gastric Cancer-Related d-Amino Acids in Saliva Based on Enzyme-Mediated Cascade Reaction

Chiral Detection of Glucose: An Amino Acid-Assisted Surface-Enhanced Raman Scattering Strategy Showing Opposite Enantiomeric Effects on SERS Signals

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