Peak Velocity as an Alternative Method for Training Prescription in Mice

Abstract

Purpose: To compare the efficiency of an aerobic physical training program prescribed according to either velocity associated with maximum oxygen uptake (vVO2max) or peak running speed obtained during an incremental treadmill test (Vpeak_K) in mice.Methods: Twenty male Swiss mice, 60 days old, were randomly divided into two groups with 10 animals each: 1. group trained by vVO2max (GVO2), 2. group trained by Vpeak_K (GVP). After the adaptation training period, an incremental test was performed at the beginning of each week to adjust training load and to determine the amount of VO2 and VCO2 fluxes consumed, energy expenditure (EE) and run distance during the incremental test. Mice were submitted to 4 weeks of aerobic exercise training of moderate intensity (velocity referring to 70% of vVO2max and Vpeak_K) in a programmable treadmill. The sessions lasted from 30 to 40 min in the first week, to reach 60 min in the fourth week, in order to provide the mice with a moderate intensity exercise, totaling 20 training sessions.Results: Mice demonstrated increases in VO2max (ml·kg−1·min−1) (GVO2 = 49.1% and GVP = 56.2%), Vpeak_K (cm·s−1) (GVO2 = 50.9% and GVP = 22.3%), EE (ml·kg−0,75·min−1) (GVO2 = 39.9% and GVP = 51.5%), and run distance (cm) (GVO2 = 43.5% and GVP = 33.4%), after 4 weeks of aerobic training (time effect, P < 0.05); there were no differences between the groups.Conclusions: Vpeak_K, as well as vVO2max, can be adopted as an alternative test to determine the performance and correct prescription of systemized aerobic protocol training to mice.

Depletion of HuR in murine skeletal muscle enhances exercise endurance and prevents cancer-induced muscle atrophy

“…Therefore, we used the Columbus Instrument’s Comprehensive Animal Monitoring System (CLAMS)® (Fig. 3a) to determine the rate of oxygen consumption (VO 2 ) and carbon dioxide production (VCO 2 ), two indicators widely used to measure the oxidative capacity of rodents and humans 20 . While we did not observe any change in the voluntary movement of these animals (Supplementary Fig.…”

Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

“…To determine the maximum power, you need to perform a treadmill test together with gas analyzer equipment and obtain the VO 2 max/VO 2 peak ( Hoydal et al., 2007 ; Petrosino et al., 2016 ). A simpler, yet less accurate, alternative is to do an incremental speed test until mice cannot run anymore and obtain the maximum velocity (Vmax), which correlates with VO 2 max/VO 2 peak ( Jordan et al., 2017 ; Picoli et al., 2018 ) . In this protocol we use a speed of 22–25 cm/s which is considered as moderate intensity (within ∼60% of their maximal velocity), as untrained C57BL/6J mice reach a Vmax on average at 40 cm/s ( Ezagouri et al., 2019 ).…”

Exercise training improves cardiac function and regulates myocardial mitophagy differently in ischaemic and pressure‐overload heart failure mice

“…(1) For the adaptation period, treadmill speed was 0.0 m/min for 15 min on day 1; 5.0 m/min for 15 min on day 2; and 10.0 m/min for 10 min on day 3 (Janice Sánchez et al., 2019). (2) For the exercise capacity test, exercise capacity was assessed by corrected peak velocity ( V peak ), where V peak = ( V + t / T × S ), and T = 180 s, S = 3.0 m/min, V is the maximum running speed at the finished phase (m/min), and t is the running time of the unfinished phase (s) (Picoli et al., 2018; Weissmann et al., 2014). All mice were tested pre‐exercise and post‐exercise, and mice undergoing training were given an additional test to adjust the exercise intensity in the mid‐exercise period.…”

“…where V peak = (V + t/T × S), and T = 180 s, S = 3.0 m/min, V is the maximum running speed at the finished phase (m/min), and t is the running time of the unfinished phase (s) (Picoli et al, 2018;Weissmann et al, 2014). All mice were tested pre-exercise and post-exercise, and mice undergoing training were given an additional test to adjust the exercise intensity in the mid-exercise period.…”