Peanut agglutinin-induced structural changes in cowpea Rhizobia as revealed by freeze-etching

Mody, Bina; Mody, Rustom; Modi, V. V.

doi:10.1007/bf02092163

Cited by 2 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such extracellular polar body consists of (lipo)polysaccharides, while the polar periplasmic bay is the place where presumably (lipo)polysaccharide synthesis takes place [146, 147]. In addition, PNL was found to enhance the incidence of extracellular polar body [145], although capsule formation also occurred without PNL as the culture grew older [140, 145]. Capsule development initiates at one pole of the cell and expands to engulf the entire cell [140].…”

Section: Molecular Communication Between the Symbiontsmentioning

confidence: 99%

“…Importantly, PNL has been shown to induce structural changes in groundnut‐nodulating bradyrhizobia: cells displayed polarity in forming an extracellular polar outgrowth of the outer membrane opposite a polar periplasmic bay [145]. Such extracellular polar body consists of (lipo)polysaccharides, while the polar periplasmic bay is the place where presumably (lipo)polysaccharide synthesis takes place [146, 147].…”

Section: Molecular Communication Between the Symbiontsmentioning

confidence: 99%

See 1 more Smart Citation

Nodulation of groundnut byBradyrhizobium: a simple infection process by crack entry

1997

View full text Add to dashboard Cite

Infection of legumes by rhizobia may occur by immediate intercellular penetration of root cells (crack entry) as an alternative mode to the more elaborate infection through infection threads. The intercellular spreading mode of infection is exemplified through a comprehensive description of root infection by Bradyrhizobium and nodule organogenesis in Arachis hypogaea (groundnut). The role of axillary root hairs and the processes of plant penetration and intercellular spreading, of internalization and intracellular multiplication, and of bacteroid differentiation are described. Then flavonoids and phytoalexins, Nod factors, lectins, and surface poly(oligo)saccharides pass in review. The roles of these various (macro)molecules in the chemical communication between the two symbionts are discussed. Attention is given to special features of groundnut nodules; the presence and functions of oleosomes and other bodies, the presence and functions of nodule lectins, and the evidence for the export of amides from the nodules are discussed. Finally, a speculative model for the groundnut infection process is presented.

show abstract

Section: Molecular Communication Between the Symbiontsmentioning

confidence: 99%

Section: Molecular Communication Between the Symbiontsmentioning

confidence: 99%

Nodulation of groundnut byBradyrhizobium: a simple infection process by crack entry

1997

View full text Add to dashboard Cite

show abstract

Systematic Evaluation of Macromolecular Carbohydrate-Lectin Recognition Using Precision Glycopolymers

Williams,

Stone,

Joshi

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

The precise modulation of protein-carbohydrate interactions is critical in glycobiology, where multivalent binding governs key cellular processes. As such, synthetic glycopolymers are useful for probing these interactions. Herein, we developed precision glycopolymers (PGPs) with unambiguous local chemical composition and well-defined global structure and systematically evaluated the effect of polymer length, hydrophobicity, and backbone hybridization as well as glycan density and identity on the binding to both mammalian and plant lectins. Our studies identified glycan density as a critical factor, with PGPs below 50% grafting density showing significantly weaker lectin interactions. Coarse-grained molecular dynamics simulations suggest that the observed phenomena may be due to a decrease in carbohydrate-carbohydrate interactions in fully grafted PGPs, leading to improved solvent accessibility. In functional assays, these PGPs reduced the cell viability and migration in 4T1 breast cancer cells. Our findings establish a structure−activity relationship in glycopolymers, providing new strategies for designing synthetic glycomacromolecules for a myriad of applications.

show abstract

Peanut agglutinin-induced structural changes in cowpea Rhizobia as revealed by freeze-etching

Cited by 2 publications

References 20 publications

Nodulation of groundnut byBradyrhizobium: a simple infection process by crack entry

Nodulation of groundnut byBradyrhizobium: a simple infection process by crack entry

Systematic Evaluation of Macromolecular Carbohydrate-Lectin Recognition Using Precision Glycopolymers

Contact Info

Product

Resources

About