The amino acid levels of oil-free peanut meal prepared from 16 varieties of peanuts that had 24-30% protein content of the kernels were examined. Nearly two-fold variations in the limiting essential amino acids (lysine, isoleucine, methionine, threonine, and valine) were found and are reported. Data for the remaining amino acids also are included. Utilization of these variations in amino acid composition should assist the development of peanut protein of improved quality. An analytical procedure to measure amino acid composition of peanut meal with a high precision on duplicate samples and which requires 15 hr for hydrolysis for best accuracy is described.