Peanut T-cell epitope discovery: Ara h 1

Ramesh, Manish; Yuenyongviwat, Araya; Konstantinou, George N.; Lieberman, Jay; Pascal, Mariona; Masilamani, Madhan; Sampson, Hugh A.

doi:10.1016/j.jaci.2015.12.1327

Cited by 42 publications

(44 citation statements)

References 30 publications

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“…Indeed, contrasting theoretical information about epitopes prediction and available experimental results (IEDB http://www.iedb.org), for allergens analysed in the current study showed that e.g. all predicted specific epitopes for Ara h 1 ( Figure 4) have been confirmed experimentally and identified as allergens ID 99142, 98985, 98887, 99364 [42][43][44], and 148649, 148770 [43,44]. Searching Lup an 1 specific epitopes (Table 1) in the Ara h 1 epitopes database IEDB allowed us to identify one experimentally confirmed epitope from Ara h 1 (ID 148579) [45,46] that matched with the Lup an 1 epitope VRRYSARLS in 7 out of 9 positions (∼78%), which may be enough to induce cross-reactivity between both proteins.…”

Section: Discussioncontrasting

confidence: 76%

“…Epitopes located in the globular region of Lup an 1 are the ones commonly shared to other legume proteins. In this regard, a large number of epitopes (thirty-six peptides) have been also identified for the peanut allergen Ara h 1 as T-cell epitopes vaccines candidates by using in silico predictions and MHC class II binding assays [44]. Several of these epitopes may be involved in cross-reactivity between Ara h 1 and Lup an 1 [44].…”

Section: Discussionmentioning

confidence: 99%

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Narrow‐leafed lupin (Lupinus angustifolius L.) β‐conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory‐related disease amelioration

Lima-Cabello

Alché

Foley

et al. 2017

Molecular Nutrition Food Res

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The use of narrow leafed lupin -NLL (Lupinus angustifolius L.) as a new food is resulting in an increasing number of allergic reactions cases, particularly in atopic patients with other pre-existing legume allergies.In the current study, we have performed an extensive in silico analysis of the NLL seed β-conglutin proteins, a new family of major allergen proteins identified in NLL, and a comparison to other relevant food allergens such as peanut Ara h 1. We analysed the variability of surface residues involved in conformational IgE-binding epitopes, lineal B-and T-cell epitopes, and changes in 2-D structural elements and 3D motives, with the aim to investigate cross-allergenicity among lupin, peanut, and other different legumes. Our results revealed that considerable structural differences exist, particularly affecting 2-D elements (loops and coils), and numerous micro-heterogeneities are present in fundamental residues directly involved in epitopes differential variability. Thus, variability of residues involved in IgE-binding epitopes might be a major contributor to the observed differences in cross-allergenicity among legumes.

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Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Narrow‐leafed lupin (Lupinus angustifolius L.) β‐conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory‐related disease amelioration

Lima-Cabello

Alché

Foley

et al. 2017

Molecular Nutrition Food Res

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“…To date, 27 linear epitopes have been recognized within the molecule of Ara h 1, of which the two most potent epitopes are found within the amino acid residues 139 to 147 and 175 to 183 (Matsuo, Yokooji, & Taogoshi, ). Recently, 36 T‐cell epitopes of Ara h 1 have been identified (Ramesh et al., ), which is useful as T‐cell epitopes may play a significant role in development of immunotherapy treatments (Tao et al., ). This information can help the food processing scientists to study the alteration in IgE binding epitopes to lower adverse events yet maintain T‐cell epitopes for immunotherapy purposes.…”

Section: Molecular Characteristics Of Peanut Allergensmentioning

confidence: 99%

Peanut Allergy: Characteristics and Approaches for Mitigation

Shah

Shi

Ashley

et al. 2019

Comp Rev Food Sci Food Safe

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Peanut allergy has garnered significant attention because of the high sensitization rate, increase in allergy, and severity of the reaction. Sufficiently reliable therapies and efficient mitigating techniques to combat peanut allergy are still lacking. Current management relies on avoiding peanuts and nuts and seeds with homologous proteins, although adverse events mostly occur with accidental ingestion. There is a need for hypoallergenic peanut products to protect sensitized individuals and perhaps serve as immunotherapeutic products. Alongside traditional practices of thermal and chemical treatment, novel processing approaches such as high‐pressure processing, pulsed ultraviolet light, high‐intensity ultrasound, irradiation, and pulsed electric field have been performed toward reducing the immunoreactivity of peanut. Covalent and noncovalent chemical modifications to proteins also have the tendency to alter peanut allergenicity. Enzymatic hydrolysis seems to be the most advantageous technique in diminishing the allergenic potential of peanut. Furthermore, the combined processing approach (hurdle technologies) such as enzymatic hydrolysis followed by, or in conjunction with, roasting, high pressure and heat, ultrasound with enzymatic treatment, or germination have shown a significant reduction of peanut immunoreactivity and may emerge as useful techniques in reducing the allergenicity of peanut and other foods. This study represents our current knowledge about the alterations in allergenic properties of peanut via different processing mechanisms as well as evaluating its future potential, geographical based data on increasing sensitization, clinical relevance, eliciting dose, and current management of peanut allergy. Furthermore, the molecular characteristics and clinical relevance of peanut allergens have been discussed.

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“…91 However, because of the increase in food allergies, attention has largely shifted away from epitope mapping of inhalant allergens to focus on food allergens that pose a serious risk of anaphylaxis. 92–97 Unfortunately, little is known about the relationship between T-cell epitopes of food allergens and asthma development, although this aspect is worth exploring, particularly among polysensitized children. As of 2010, only approximately 17% of all allergens listed by the International Union of Immunological Societies database had been mapped, indicating much work remains to be done.…”

Section: Tslpmentioning

confidence: 99%

Pathogenic CD4 + T cells in patients with asthma

Muehling

Lawrence

Woodfolk

2017

Journal of Allergy and Clinical Immunology

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Asthma encompasses a variety of clinical phenotypes that involve distinct T cell–driven inflammatory processes. Improved understanding of human T-cell biology and the influence of innate cytokines on T-cell responses at the epithelial barrier has led to new asthma paradigms. This review captures recent knowledge on pathogenic CD4+ T cells in asthmatic patients by drawing on observations in mouse models and human disease. In patients with allergic asthma, TH2 cells promote IgE-mediated sensitization, airway hyperreactivity, and eosinophilia. Here we discuss recent discoveries in the myriad molecular pathways that govern the induction of TH2 differentiation and the critical role of GATA-3 in this process. We elaborate on how cross-talk between epithelial cells, dendritic cells, and innate lymphoid cells translates to T-cell outcomes, with an emphasis on the actions of thymic stromal lymphopoietin, IL-25, and IL-33 at the epithelial barrier. New concepts on how T-cell skewing and epitope specificity are shaped by multiple environmental cues integrated by dendritic cell “hubs” are discussed. We also describe advances in understanding the origins of atypical TH2 cells in asthmatic patients, the role of TH1 cells and other non-TH2 types in asthmatic patients, and the features of T-cell pathogenicity at the single-cell level. Progress in technologies that enable highly multiplexed profiling of markers within a single cell promise to overcome barriers to T-cell discovery in human asthmatic patients that could transform our understanding of disease. These developments, along with novel T cell–based therapies, position us to expand the assortment of molecular targets that could facilitate personalized treatments.

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Peanut T-cell epitope discovery: Ara h 1

Abstract: Preselection of peptides by using in silico and in vitro approaches in combination with conventional methods appears to be an effective strategy for identifying peanut T-cell peptide vaccine candidates.

Cited by 42 publications

References 30 publications

Narrow‐leafed lupin (Lupinus angustifolius L.) β‐conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory‐related disease amelioration

Narrow‐leafed lupin (Lupinus angustifolius L.) β‐conglutin proteins modulate the insulin signaling pathway as potential type 2 diabetes treatment and inflammatory‐related disease amelioration

Peanut Allergy: Characteristics and Approaches for Mitigation

Pathogenic CD4 + T cells in patients with asthma

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