Pear pomace soluble dietary fiber ameliorates the negative effects of high-fat diet in mice by regulating the gut microbiota and associated metabolites

Abstract

The gut microbiota and related metabolites are positively regulated by soluble dietary fiber (SDF). In this study, we explored the effects of SDF from pear pomace (PP) on the regulation of gut microbiota and metabolism in high-fat-diet-fed (HFD-fed) C57BL/6J male mice. The results showed that PP-SDF was able to maintain the HFD disrupted gut microbiota diversity with a significant increase in Lachnospiraceae_UCG-006, Akkermansia, and Bifidobacterium spp. The negative effects of high-fat diet were ameliorated by PP-SDF by regulating lipid metabolisms with a significant increase in metabolites like isobutyryl carnitine and dioscoretine. Correlation analysis revealed that gut microbiota, such as Akkermansia and Lachnospiraceae_UCG-006 in the PP-SDF intervention groups had strong positive correlations with isobutyryl carnitine and dioscoretin. These findings demonstrated that PP-SDF interfered with the host's gut microbiota and related metabolites to reduce the negative effects caused by a high-fat diet.

Synbiotic Combination between Lactobacillus paracasei VL8 and Mannan-Oligosaccharide Repairs the Intestinal Barrier in the Dextran Sulfate Sodium-Induced Colitis Model by Regulating the Intestinal Stem Cell Niche

“…Mice cecum contents were collected and immediately stored at −80 °C to determine the composition of the gut microbiota. DNA extraction, amplification, and 16S rRNA gene sequencing were conducted according to a previous description with a slight modification . Total DNA in the feces was extracted using a DNeasy PowerSoil Pro Kit (Qiagen, the U.S.).…”

“…Mass spectrometry conditions: Positive and negative ion scan modes and mass scanning range from 70 to 1050 m / z . Specific conditions were referred to those described in the previous literature with slight modifications. , …”

From the past to the future: Fermented milks and their health effects against human diseases

“…They are not digested and absorbed by the small intestine and can mostly enter the large intestine intact to be fermented by gut microbiota and converted into more beneficial metabolites (Simpson & Campbell, 2015). Ji et al (2022) found that pear pomace dietary fiber can improve lipid metabolism by enriching beneficial bacteria Akkermansia and bifidobacteriaceae to produce more isobutyryl carnitine and phenylalanyl-valine and other key metabolites. Dietary fiber can be utilized by many beneficial bacteria, such as Lactobacillus and Bifidobacterium, and this prebiotic property makes dietary fiber well suited for application in fermented milk (Clemens, 2015).…”

“…Ji et al. (2022) found that pear pomace dietary fiber can improve lipid metabolism by enriching beneficial bacteria Akkermansia and bifidobacteriaceae to produce more isobutyryl carnitine and phenylalanyl‐valine and other key metabolites. Dietary fiber can be utilized by many beneficial bacteria, such as Lactobacillus and Bifidobacterium , and this prebiotic property makes dietary fiber well suited for application in fermented milk (Clemens, 2015).…”

Modulating the Human Gut Microbiota through Hypocaloric Balanced Diets: An Effective Approach for Managing Obesity

“…The gut microbiota plays a key role in human health by improving digestion and regulating the immune system, and thus this aspect has attracted growing concerns in recent years [ 4 ]. There is increasing evidence that imbalances in the gut microbiota may be a crucial factor in obesity [ 5 , 6 , 7 , 8 ]. Several studies have indicated that gut-microbiota-derived metabolites play a crucial role in nutrient uptake and energy metabolism of the body, and any irregularities in this process lead to obesity or being underweight [ 9 , 10 ].…”