Pearl Grey Shading Net Boosts the Accumulation of Total Carotenoids and Phenolic Compounds That Accentuate the Antioxidant Activity of Processing Tomato

Formisano, Luigi; Ciriello, Michele; El‐Nakhel, Christophe; Poledica, Milena M.; Starace, Giuseppe; Graziani, Giulia; Ritieni, Alberto; Pascale, Stefania De; Rouphael, Youssef

doi:10.3390/antiox10121999

Cited by 14 publications

(13 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The activity values for GT, SGT, WT, SWT, BT, and SBT were 2.16 ± 0.04, 2.23 ± 0.02, 1.26 ± 0.02, 1.43 ± 0.02, 0.70 ± 0.04, and 0.87 ± 0.02 mg Trolox/g-FW, respectively, based on DPPH radical scavenging (Figure C). The reduction of solar radiation through preharvest shading proved to be an excellent tool to increase tomato fruit phytochemical quality and DPPH antioxidant activities during summer cultivation . Additionally, we investigated the potential for processing of shaded tea extract into functional products by measuring the in vitro hypoglycemic activity of shaded tea extracts using α-amylase and trypsin inhibition assays .…”

Section: Resultsmentioning

confidence: 99%

“…The reduction of solar radiation through preharvest shading proved to be an excellent tool to increase tomato fruit phytochemical quality and DPPH antioxidant activities during summer cultivation. 7 Additionally, we investigated the potential for processing of shaded tea extract into functional products by measuring the in vitro hypoglycemic activity of shaded tea extracts using α-amylase and trypsin inhibition assays. 21 The inhibition rates for α-amylase and trypsin using fixed fresh leaves as the control are shown in Figure S6B,C.…”

Section: Profiles Of Antioxidant Activity and Hypoglycemicmentioning

confidence: 99%

“…6 A study found that preharvest shading increased the content of organic acids, phenols, carotenoids, and antioxidant activity in tomatoes processed in summer. 7 Our previous study elaborated that preharvest shading can promote the content of amino acids, chlorophyll, and aromatic substances and reduce the tea polyphenol content to facilitate tea appearance, color, taste, aroma, and other aspects. 8 However, as most studies focus on metabolite changes in shaded fresh leaves, it is unclear how the quality of tea products changes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Preharvest Shading on Dynamic Changes in Metabolites, Gene Expression, and Enzyme Activity of Three Tea Types during Processing

Shao

Deng

Liu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Preharvest shading significantly influences tea flavor. However, little attention has been given to the mechanism of shading on metabolites, genes, and enzymes in the processing of different tea types. Our study identified 1028 nonvolatile metabolites covering 10 subclasses using a widely targeted metabolome. The results show that shading had a greater effect on the compositions of amino acids, flavonoids, and theaflavins in tea leaves. The combined transcriptomics and enzyme activity analysis results indicate that the upregulated expression of asparagine, aspartate, and tryptophan synthesis genes and proteolytic enzymes promoted the accumulation of amino acids. The downregulated enzyme genes resulted in the reduction of nongalloylated catechins and flavonoid glycosides. Simultaneously, the accumulation of TFs in shaded tea was due to the enhanced enzymatic activities of polyphenol oxidase and peroxidase during processing. Theaflavin-3-3′-di-O-gallate was also significantly positively correlated with the antioxidant and hypoglycemic activities of shaded tea. The results contribute to a better understanding of how preharvest treatments influence summer tea quality.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Profiles Of Antioxidant Activity and Hypoglycemicmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Preharvest Shading on Dynamic Changes in Metabolites, Gene Expression, and Enzyme Activity of Three Tea Types during Processing

Shao

Deng

Liu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Pigments (total chlorophyll, a, b, and carotenoids) were determined as described by Formisano et al (2021b) . Briefly, 0.5 g of fresh leaves were extracted in ammonia acetone, crushed in a ceramic mortar, and centrifuged at 2,000 rpm for 10 min using an R-10 M centrifuge (Remi Elektrotechnik Limited, Mumbai, India).…”

Section: Methodsmentioning

confidence: 99%

Between Light and Shading: Morphological, Biochemical, and Metabolomics Insights Into the Influence of Blue Photoselective Shading on Vegetable Seedlings

et al. 2022

Self Cite

View full text Add to dashboard Cite

High nursery densities reduce the seedling quality due to the competition for light. High light intensity, shading, and blue light depletion activate morphophysiological and metabolomic responses in plants, resulting in size modification to gain an advantage over neighboring plants. Our research aimed to unravel the effects of light intensity and quality on nursery seedlings at the morphological and biochemical levels. To this aim, the effect of black shading and blue photoselective shading nets were investigated in terms of morphometric, ionomic, and untargeted metabolomics signatures in Cucurbita pepo L., Citrullus lanatus L., Solanum lycopersicum L., and Solanum melongena L. seedlings. Plant height, diameter, sturdiness index, leaf area, specific leaf area, shoot/root ratio, and mineral content (by ion chromatography-IC) were evaluated. In C. pepo L and C. lanatus L., the blue net reduced the shoot/root and chlorophyll a/b ratios and increased stem diameter and total chlorophyll content. The black net increased plant height, stem diameter, and sturdiness index in Solanum lycopersicum L. and Solanum melongena L. At the same time, unshading conditions reduced leaf area, specific leaf area, shoot/root ratio, and total chlorophyll content. The blue net improved the sturdiness index and quality of C. pepo L. and C. lanatus L. Such impact on morphological parameters induced by the different shading conditions was corroborated by a significant modulation at the metabolomics level. Untargeted metabolomic phytochemical signatures of the selected plants, and the subsequent multivariate analysis coupled to pathway analysis, allowed highlighting a broad and diverse biochemical modulation. Metabolomics revealed that both primary and secondary metabolism were largely affected by the different shading conditions, regardless of the species considered. A common pattern arose to point at the activation of plant energy metabolism and lipid biosynthesis, together with a generalized down accumulation of several secondary metabolites, particularly phenylpropanoids. Our findings indicate an intriguing scientific interest in the effects of selective shading and its application to other species and different phenological stages.

show abstract

“…The antioxidant activities ABTS + (2,2′-azinobis-(3ethylbenzothiazoline-6-sulfonate), DPPH (2,2-diphenyl-1picrylhydrazyl), and FRAP (ferric reduction antioxidant potency) were determined by UV-VIS spectrophotometry (Shimadzu, Japan) according to the protocols described by Formisano et al (2021). Results were expressed as mmol Trolox equivalents kg -1 dw.…”

Section: Abts Dpph and Frap Antioxidant Activities Determinationmentioning

confidence: 99%

Zinc biofortification of hydroponically grown basil: Stress physiological responses and impact on antioxidant secondary metabolites of genotypic variants

Ciriello

Formisano²,

Kyriacou³

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Ocimum basilicum L. is an aromatic plant rich in bioactive metabolites beneficial to human health. The agronomic biofortification of basil with Zn could provide a practical and sustainable solution to address Zn deficiency in humans. Our research appraised the effects of biofortification implemented through nutrient solutions of different Zn concentration (12.5, 25.0, 37.5, and 50 µM) on the yield, physiological indices (net CO2 assimilation rate, transpiration, stomatal conductance, and chlorophyll fluorescence), quality, and Zn concentration of basil cultivars ‘Aroma 2’ and ‘Eleonora’ grown in a floating raft system. The ABTS, DPPH, and FRAP antioxidant activities were determined by UV-VIS spectrophotometry, the concentrations of phenolic acids by mass spectrometry using a Q Extractive Orbitrap LC-MS/MS, and tissue Zn concentration by inductively coupled plasma mass spectrometry. Although increasing the concentration of Zn in the nutrient solution significantly reduced the yield, this reduction was less evident in ‘Aroma 2’. However, regardless of cultivar, the use of the maximum dose of Zn (50 µM) increased the concentration of carotenoids, polyphenols, and antioxidant activity on average by 19.76, 14.57, and 33.72%, respectively, compared to the Control. The significant positive correlation between Zn in the nutrient solution and Zn in plant tissues underscores the suitability of basil for soilless biofortification programs.

show abstract

Pearl Grey Shading Net Boosts the Accumulation of Total Carotenoids and Phenolic Compounds That Accentuate the Antioxidant Activity of Processing Tomato

Cited by 14 publications

References 76 publications

Effects of Preharvest Shading on Dynamic Changes in Metabolites, Gene Expression, and Enzyme Activity of Three Tea Types during Processing

Effects of Preharvest Shading on Dynamic Changes in Metabolites, Gene Expression, and Enzyme Activity of Three Tea Types during Processing

Between Light and Shading: Morphological, Biochemical, and Metabolomics Insights Into the Influence of Blue Photoselective Shading on Vegetable Seedlings

Zinc biofortification of hydroponically grown basil: Stress physiological responses and impact on antioxidant secondary metabolites of genotypic variants

Contact Info

Product

Resources

About