PEBP Signaling Network in Tubers and Tuberous Root Crops

Susila, Hendry; Purwestri, Yekti Asih

doi:10.3390/plants12020264

Cited by 11 publications

(8 citation statements)

References 132 publications

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“…Indeed, the development of storage roots in at least some root crop species is dependent on the photoperiod [224]. On the other hand, several copies of FT-TFL1-like genes closely related to potato SP6A have been identified in the selected root crops, including cassava, sweet potato, radish, carrot and sugar beet [225,226], suggesting their role in storage root development.…”

Section: Facs In the Regulation Of Storage Organ Formationmentioning

confidence: 99%

Functional Modules in the Meristems: “Tinkering” in Action

Kuznetsova,

Efremova,

Dodueva

et al. 2023

Plants

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Background: A feature of higher plants is the modular principle of body organisation. One of these conservative morphological modules that regulate plant growth, histogenesis and organogenesis is meristems—structures that contain pools of stem cells and are generally organised according to a common principle. Basic content: The development of meristems is under the regulation of molecular modules that contain conservative interacting components and modulate the expression of target genes depending on the developmental context. In this review, we focus on two molecular modules that act in different types of meristems. The WOX-CLAVATA module, which includes the peptide ligand, its receptor and the target transcription factor, is responsible for the formation and control of the activity of all meristem types studied, but it has its own peculiarities in different meristems. Another regulatory module is the so-called florigen-activated complex, which is responsible for the phase transition in the shoot vegetative meristem (e.g., from the vegetative shoot apical meristem to the inflorescence meristem). Conclusions: The review considers the composition and functions of these two functional modules in different developmental programmes, as well as their appearance, evolution and use in plant breeding.

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Section: Facs In the Regulation Of Storage Organ Formationmentioning

confidence: 99%

Functional Modules in the Meristems: “Tinkering” in Action

Kuznetsova,

Efremova,

Dodueva

et al. 2023

Plants

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“…Despite the above knowledge, little is known about the genetic regulation of bulbil formation in P. ternata . In angiosperms, the PEBP (Phosphatidylethanolamine-Binding) gene family plays an important role in organ development, e.g., floral initiation and underground storage organ formation [ 30 ]. The transcriptome and profiles of Pinellia cordata were analyzed, and a total of fourteen unigenes homologous to PEBP gene family members were identified; there were four unigenes with differential expressions in three kinds of tissue (petiole, bulbil, and tuber), as revealed by the profiles, which indicates that members of the PEBP gene family could be involved in the bulbil development of Pinellia cordata [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Mou,

Zhang,

Qiu

et al. 2024

IJMS

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Pinellia ternata is a medicinal plant that has important pharmacological value, and the bulbils serve as the primary reproductive organ; however, the mechanisms underlying bulbil initiation remain unclear. Here, we characterized bulbil development via histological, transcriptomic, and targeted metabolomic analyses to unearth the intricate relationship between hormones, genes, and bulbil development. The results show that the bulbils initiate growth from the leaf axillary meristem (AM). In this stage, jasmonic acid (JA), abscisic acid (ABA), isopentenyl adenosine (IPA), and salicylic acid (SA) were highly enriched, while indole-3-acetic acid (IAA), zeatin, methyl jasmonate (MeJA), and 5-dexoxystrigol (5-DS) were notably decreased. Through OPLS-DA analysis, SA has emerged as the most crucial factor in initiating and positively regulating bulbil formation. Furthermore, a strong association between IPA and SA was observed during bulbil initiation. The transcriptional changes in IPT (Isopentenyltransferase), CRE1 (Cytokinin Response 1), A-ARR (Type-A Arabidopsis Response Regulator), B-ARR (Type-B Arabidopsis Response Regulator), AUX1 (Auxin Resistant 1), ARF (Auxin Response Factor), AUX/IAA (Auxin/Indole-3-acetic acid), GH3 (Gretchen Hagen 3), SAUR (Small Auxin Up RNA), GA2ox (Gibberellin 2-oxidase), GA20ox (Gibberellin 20-oxidase), AOS (Allene oxide synthase), AOC (Allene oxide cyclase), OPR (Oxophytodienoate Reductase), JMT (JA carboxy l Methyltransferase), COI1 (Coronatine Insensitive 1), JAZ (Jasmonate ZIM-domain), MYC2 (Myelocytomatosis 2), D27 (DWARF27), SMAX (Suppressor of MAX2), PAL (Phenylalanine Ammonia-Lyase), ICS (Isochorismate Synthase), NPR1 (Non-expressor of Pathogenesis-related Genes1), TGA (TGACG Sequence-specific Binding), PR-1 (Pathogenesis-related), MCSU (Molybdenium Cofactor Sulfurase), PP2C (Protein Phosphatase 2C), and SnRK (Sucrose Non-fermenting-related Protein Kinase 2) were highly correlated with hormone concentrations, indicating that bulbil initiation is coordinately controlled by multiple phytohormones. Notably, eight TFs (transcription factors) that regulate AM initiation have been identified as pivotal regulators of bulbil formation. Among these, WUS (WUSCHEL), CLV (CLAVATA), ATH1 (Arabidopsis Thaliana Homeobox Gene 1), and RAX (Regulator of Axillary meristems) have been observed to exhibit elevated expression levels. Conversely, LEAFY demonstrated contrasting expression patterns. The intricate expression profiles of these TFs are closely associated with the upregulated expression of KNOX(KNOTTED-like homeobox), suggesting a intricate regulatory network underlying the complex process of bulbil initiation. This study offers a profound understanding of the bulbil initiation process and could potentially aid in refining molecular breeding techniques specific to P. ternata.

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“…The mL38 mitoribosomal protein family forms a subgroup of a larger, diverse family of proteins known as the PEBP (Phosphatidylethanolamine Binding Protein) protein family, composed largely of nonmitochondrial, nonribosomal protein members ( Serre et al. , 1998 , 2001; Tsoy and Mushegian, 2022 ; Susila and Purwestri, 2023 ). PEBP proteins are found in many bacteria, archaea and broadly in eukaryotes, and include proteins such as the Raf-kinase inhibitor protein (RKIP) in mammals, and the flowering locus T (FT) protein in plants ( Banfield and Brady, 2000 ; Chautard et al.…”

Section: Introductionmentioning

confidence: 99%

Mutation of the PEBP-like domain of the mitoribosomal MrpL35/mL38 protein results in production of nascent chains with impaired capacity to assemble into OXPHOS complexes

Box,

Anderson,

Stuart

2023

MBoC

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Located in the central protuberance region of the mitoribosome, mitospecific mL38 proteins display homology to PEBP (phosphatidylethanolamine binding protein) proteins, a diverse family of proteins reported to bind anionic substrates/ligands and implicated in cellular signaling and differentiation pathways. In this study, we have performed a mutational analysis of the yeast mitoribosomal protein MrpL35/mL38 and demonstrate that mutation of the PEBP-invariant ligand binding residues Asp(D)232 and Arg(R)288 impacted MrpL35/mL38’s ability to support OXPHOS-based growth of the cell. Furthermore, our data indicate these residues exist in a functionally important charged microenvironment, which also includes Asp(D)167 of MrpL35/mL38 and Arg(R)127 of the neighboring Mrp7/bL27m protein. We report that mutation of each of these charged residues resulted in a strong reduction in OXPHOS complex levels that was not attributed to a corresponding inhibition of the mitochondrial translation process. Rather, our findings indicate that a disconnect exists in these mutants between the processes of mitochondrial protein translation and the events required to ensure the competency and/or availability of the newly synthesized proteins to assemble into OXPHOS enzymes. Based on our findings, we postulate that the PEBP-homology domain of MrpL35/mL38, together with its partner Mrp7/bL27m, form a key regulatory region of the mitoribosome.

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PEBP Signaling Network in Tubers and Tuberous Root Crops

Cited by 11 publications

References 132 publications

Functional Modules in the Meristems: “Tinkering” in Action

Functional Modules in the Meristems: “Tinkering” in Action

Endogenous Hormone Levels and Transcriptomic Analysis Reveal the Mechanisms of Bulbil Initiation in Pinellia ternata

Mutation of the PEBP-like domain of the mitoribosomal MrpL35/mL38 protein results in production of nascent chains with impaired capacity to assemble into OXPHOS complexes

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