Pectic Enzymes: Individual and Concerted Kinetic Behavior of Pectinesterase and Pectinase

Purification and Characterization of Pectinmethylesterase from Ficus awkeotsang Makino Achenes

“…The maximal activity was higher at higher NaCl concentration. However, the stimulation effect of NaCl is not so great as that reported for alfalfa (17) and tomato (7). The jelly fig PME appears to be a monomer with a mol wt of 38,000.…”

A comparative systems analysis of polysaccharide‐elicited responses in Neurospora crassa reveals carbon source‐specific cellular adaptations

“…One or both GH28 enzymes further relies on the pectin methylesterase CE8‐1 to modify the substrate, since the absence of this non‐hydrolytic enzyme also leads to a similar phenotype. To our knowledge, this is the first report of an in vivo synergism between these enzymes in saprobic fungi, which has previously been observed only in vitro (Jansen et al ., ; Dahodwala et al ., ; Pressey and Avants, ; Christgau et al ., ; Massiot et al ., ; Benen et al ., ; Wakabayashi et al ., ). In plant pathogenic fungi, several polygalacturonase or pectin methylesterase enzymes have been demonstrated to be important for growth on pectin and (often) essential for virulence (Schell et al ., ; Scott‐Craig et al ., ; Isshiki et al ., ; Valette‐Collet et al ., ; Moran‐Diez et al ., ).…”

A Review: Maceration in Relation to the Post‐harvest Handling and Processing of Plant Material

“…While PE will greatly increase the extent of degradation of isolated pectinic acids by depolymerases preferentially active against the de-esterified galacturonan (Dahodwala et al 1974;, there is little actual evidence to suggest that its action is significant during the maceration of dicotyledons by micro-organisms. Additions of either endo-PG or endo-PAL to plant tissue, in the absence of other added activities, will result in cell separation.…”