Pectin-like polysaccharide extracted from the leaves of Conocarpus erectus Linnaeus promotes antioxidant, immunomodulatory and prebiotic effects

Santos, Dayane Kelly Dias do Nascimento; Barros, Bárbara Rafaela da Silva; Filho, Iranildo José da Cruz; Júnior, Natanael da Silva Bezerra; Silva, Paula Roberta da; Nascimento, Pedro Henrique do Bomfim; Lima, Maria do Carmo Alves de; Napoleão, Thiago Henrique; Melo, Cristiane Moutinho Lagos de

doi:10.1016/j.bcdf.2021.100263

Cited by 13 publications

(21 citation statements)

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“…The solid pectin precipitate was dried at 70°C for approximately 72 hours according to the methodology of Habibi and coauthors [13]. The yield for obtaining pectin, ie precipitation in ethanol, was determined by Equation 1 proposed by Santos and coauthors [3].…”

Section: Extraction Of Pectin From C Pulcherrima Leavesmentioning

confidence: 99%

“…The methodology used to determine the moisture and ash contents was proposed by Santos and coauthors [3]. For moisture determination, the constant weight methodology was used, where 2.0 g of pectin were placed in an oven (Tecnal, TE-393/1), at 105 ± 2ºC and weighed at regular intervals of time, until weight constant.…”

Section: Determination Of Moisture and Ash Content Present In Pectinmentioning

confidence: 99%

“…Pectins refer to a family of oligosaccharides and polysaccharides, with common characteristics, however extremely diverse in structure [1]. The pectic skeleton is formed by α-linked galacturonic acid units (1→4), with varying degrees of esterified methyl carboxyl groups [2,3]. This skeleton can have different side chains, formed by different neutral sugars, such as galactose, rhamnose, arabinose, fucose, among others [2,1].…”

Section: Introductionmentioning

confidence: 99%

“…The diversity of composition and molecular arrangement of pectins enhance their biological properties. In the literature, pectins have an anti-inflammatory and analgesic action [6], gastroprotective [7], immunomodulatory [3], antioxidant activity [3,6], pro-inflammatory activity [3] and anti-tumor activity [8]. These biological activities are directly related to the chemical structure of these polysaccharides, that is, molecular weight, degree of esterification, solubility, free hydroxyl and galacturonic acid contents and different sugars present in the structure [9].…”

Section: Introductionmentioning

confidence: 99%

“…In Brazil, the species found the necessary conditions for the development of its fruits and seeds, which are produced in large quantities throughout the year. In folk medicine it is used in the treatment of various diseases, such as fevers, infections and oral ulcers [3,4]. In the pharmacological area, extracts obtained from flowers, fruits, seeds, bark, roots and leaves have been used in different activities, such as analgesics, anti-inflammatory [5], antioxidants, immunomodulators [6], anti-ulcer [7], and antifungal activity [10].…”

Section: Introductionmentioning

confidence: 99%

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Pectin-like Polysaccharide Extracted from the Leaves Caesalpinia pulcherrima is a Promising Antioxidant and Immunomodulator Agent

Melo

Sousa

Silva

et al. 2022

Braz. arch. biol. technol.

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Studies have shown that Caesalpinia pulcherrima extracts promote antioxidant, healing, immunomodulating and antiparasitic activities and its polysaccharides can be used as functional food. In this sense, this work had as objective the isolation and characterization of a polysaccharide-like pectin, extracted from the C. pulcherrima leaves and its possible applications as an antioxidant and immunomodulator agent. The molecule was characterized by high performance liquid chromatography, fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Its antioxidant potential was evaluated through the methods of phosphomolybdenum, ABTS radical scavenging [2,2'-azino-bis (3-ethylbenzothiazoline-6sulfonic acid], DPPH (1,1-diphenyl-2-picrylhydrazyl) and nitric oxide radical. The immunostimulating effects of pectin were tested in splenocytes to evaluate its toxic, proliferative and cell activator and immunomodulatory potential. The polysaccharide obtained has structural characteristics similar to pectins. Pectin showed high in vitro antioxidant activity for ABTS radical scavenging, moderate activity for phosphomolybdenum and low activity for DPPH and nitric oxide. In vitro immunomodulation assays showed that pectin obtained did not promote a cytotoxic effect (viability > 90%). The increase in cytosolic ROS levels indicates a possible mechanism of cell activation without causing damage. Immunophenotyping showed that HIGHLIGHTS• The pectin-like polysaccharide was isolated from the Caesalpinia pulcherrima leaves.• Showed promising results of antioxidant activity in in vitro assays.• The polysaccharide is non-toxic, stimulates cell activation and cytokine production in in vitro.• Promising therapeutic agent.

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Section: Extraction Of Pectin From C Pulcherrima Leavesmentioning

confidence: 99%

Section: Determination Of Moisture and Ash Content Present In Pectinmentioning

confidence: 99%