Pectinase production by Aspergillus niger using wastewater in solid state fermentation for eliciting plant disease resistance

Abstract

An elicitor of plant disease resistance, pectinase, was produced by solid state fermentation with Aspergillus niger. Sugar beet pulp was used as carbon source and the wastewater from monosodium glutamate production was used as nitrogen and water source. The composition of the fermentation medium was: 11 ml concentrated wastewater (containing NH 3 -N 38.2 mg/ml), sugar beet pulp 10 g, Na 2 HPO 4 Á 12H 2 O 0.2 g, KH 2 PO 4 0.04 g in a 500 ml Erlenmeyer flask. The fermentation temperature was 30°C and the relative humidity of the air was 75-90%. The maximum production of pectinase was reached after 96 h cultivation. The crude pectinase extracted from the fermented materials could elicit disease resistance in cucumber and tomato seedlings.

Column bioreactor use for optimization of pectinase production in solid substrate cultivation

“…They found A. niger as the best producer among the tested microorganisms. Besides that enzymatic extracts of A. niger have been used safely in food (10) and feed (17) industry and another applications as model to study the interaction between protein and carbohydrate (11) and to promote plant protection (2). Thus, the use of A. niger was chosen to the next experimental phase, and one variable was ruled out.…”

Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

“…PW is also a high concentration of COD wastewater. A few studies have focused on the utilization of such high concentration of organic matter wastewater (Bai et al, 2004;Mishra et al, 2004;Huang et al, 2005). As far as the authors are aware, no article has been published on utilization of both MGW and PW in one process.…”

“…Effluents from potato chips industry possess a high load of starchy materials (Mishra et al, 2004). Because of the low pH, high concentration of COD, sulfate and NH 3 -N, the treatment of such wastewaters by conventional activated sludge processes consume a lot of energy, resulting in high treatment costs (Bai et al, 2004).…”

Utilization of Sweat Potato Starch Wastewater and Monosodium Glutamate Wastewater for Cultivation of an Anti-Fungal Biocontrol Agent Paenibacillus Polymyxa

“…SPW also contains high COD and nutrient loadings. A few studies have already focused on the utilization of such high organic loading wastewater as substrates or production media in fermentation processes (Bai et al, 2004;Mishra et al, 2004;Huang et al, 2005). As far as the authors are aware, no papers have been published on utilization of both MGW and SPW in one process.…”

“…Effluents from sweat potato starch industry contains a high load of protein, pectin, and starchy materials with the COD of 10,000 -35,000 mg/l (Mishra et al, 2004). Due to the low pH, high concentration of COD, sufate and NH 3 -N, the treatment of such wastewaters by conventional activated sludge processes consumes a lot of energy, resulting in high treatment costs (Bai et al, 2004). Phyllosphere bacteria often have a positive influence upon plant, where they may be involved in the fixation of nitrogen, promoting the growth of plants, or the control of plant pathogens.…”