Pectobacterium carotovorum subsp. carotovorum - the causal agent of broccoli soft rot in Serbia

Gašić, Katarina; Gavrilović, Veljko; Dolovac, Nenad; Trkulja, Nenad; Živković, Svetlana; Ristić, Danijela; Obradović, Aleksa

doi:10.2298/pif1404249g

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…It has been widely reported that P. fluorescens and P. carotovorum are responsible for head soft rot in broccoli (Gašić et al, 2014). However, in the present work, S. liquefaciens , together with Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…Different species of bacteria can infect the florets of broccoli and produce head soft rot. Soft rot is caused by pectolytic bacteria capable of producing pectic enzymes, such as Pectobacterium carotovorum (formerly Erwinia carotovora ; Gašić et al, 2014), Pseudomonas fluorescens , Pseudomonas marginalis , and Pseudomonas brassicae (Aremu & Babalola, 2015). More recently, Clostridium spp., Cytophaga spp., and Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Amaranth cystatin inhibits the growth of broccoli (Brassica oleracea var. italica L.) pathogenic bacteria

Manzo‐Valencia

Cervantes‐Juan

Mozqueda‐Cervantes

et al. 2023

Journal of Phytopathology

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Head soft rot is the major disease of broccoli caused by Pseudomonas fluorescens and Pectobacterium carotovorum (formerly Erwinia carotovora). However, in the present work, Pseudomonas sp. was isolated along with Pantoea agglomerans and Serratia liquefaciens, from broccoli heads with disease symptoms. Different phytopathogenic assays confirmed the ability of the isolated Serratia liquefaciens strain to produce soft rot disease in stems and florets of broccoli. This study also investigated the effect of an amaranth cystatin (AhCPI) on the growth of these three bacterial pathogens and on the development of soft rot symptoms in broccoli. AhCPI significantly reduced the severity of head soft rot symptoms caused by Pseudomonas sp., and S. liquefaciens in vitro. Bacterial growth inhibition increased with the increase in AhCPI concentration. In the same way, it was demonstrated that the application of AhCPI (10 or 20 μM) on florets of broccoli with soft rot symptoms significantly reduced the severity of disease caused by S. liquefaciens or Pseudomonas sp. Our results suggest that S. liquefaciens can produce head soft rot in florets of broccoli and AhCPI could be a new sustainable alternative for the control of head soft rot disease in broccoli.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amaranth cystatin inhibits the growth of broccoli (Brassica oleracea var. italica L.) pathogenic bacteria

Manzo‐Valencia

Cervantes‐Juan

Mozqueda‐Cervantes

et al. 2023

Journal of Phytopathology

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“…atrosepticumабо Dickeya spp. не утворюють кислоти з α-метил глюкозиду та мальтози, але утилізують лактозу і трегалозу, і не продукують індол або лецитиназу [24].…”

Section: морфологічні характеристики колоній вірулентних ізолятівunclassified

Вacterial rot of tomatoes when grown in a protected ground

Bohoslavets

Kolomiiets²,

Butsenko³

et al. 2020

Biological Systems: Theory and Innovation

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The specific conditions of the protected ground, the almost constant tomato culture without substitution of the substrate, the elevated temperature and humidity lead to the accumulation of a large number of pathogens of bacterial diseases, which limit the increase in yield of this crop. The aim of the work was to characterize the symptoms, determine the etiology of wet rot of tomatoes for growing in protected ground and propose measures to control the pathogen. The study was conducted by standard microbiological and phytopathological methods. The pathogenic properties of the isolates were studied on vegetative tomato plants using a suspension of bacterial cells with a titer of 107 CFU/ml. It was established that the defeat of tomato plants with soft bacterial rot in closed ground conditions prevails in the second half of the growing season. The development of the disease was 30‒34% for a prevalence of 45%. Diseases of tomato plants in greenhouses in the Kiev region of Ukraine are of bacterial origin, caused by the soft rot pathogen P. carotovorum subsp. carotovorum. Characteristic symptoms of wet tomato rot are discoloration, chlorosis and leaf necrosis, void stems, the appearance of depressed water-saturated areas in the stalk, accompanied by decay of the fetus. Effective control measures can be preventive and agricultural measures

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“…Therefore, the soft rot test on potatoes was employed to identify the pectolytic Pectobacterium , but Pseudomonas was also reported to possess the same activity (Krejzar et al, 2008). Therefore, 16S rDNA sequences have been widely used and served as a gold standard technique for identifying P. carotovorum (Gasic et al, 2013; Hu et al, 2008; Lee et al, 2014). In addition, nucleotide sequences of the pectate lyase gene ( pel ) were also employed for the taxonomic identification of P. carotovorum (Baghaee‐Ravari et al, 2011; Faquihi et al, 2015; Golkhandan et al, 2013; Nazerian et al, 2011; Terta et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Host range and virulence diversity of Pectobacterium carotovorum subsp. brasiliense strain RDKLR infecting radish in India, and development of a LAMP-based diagnostics

Chandrashekar

Prasannakumar

Parivallal

et al. 2022

Journal of Applied Microbiology

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Aim This work aimed at determining the pathogenicity, molecular characterization, host range and rapid detection of Pectobacterium carotovorum subsp. brasiliense (Pcb) causing soft rot disease in radish. Methods and Results The four isolated isolates were inoculated to radish, typical soft rot symptoms were observed and Koch’s postulates were proved. The most virulent strain RDKLR was morphologically and biochemically distinct. Pcb showed a positive potato soft rot test and elicited hypersensitivity response on Nicotiana tobaccum. The genes Pel2 and pmrA were used for subspecies characterization of Pcb. It has a wide host range and infection was observed on slices of carrot, tomato, radish, potato, cauliflower, cabbage, chilli, knol‐khol, bell pepper and cucumber. Infectivity was also seen in seedlings under glasshouse conditions. Pcb produced cell wall degrading enzymes in semi‐quantification assay and is a strong biofilm producer. The LAMP technique was standardized to help rapid detection and take prophylactic measures to manage the disease. Conclusion This work reports Pcb as a new soft rot causing organism of radish in India. Pcb is highly virulent with a broad host range. The LAMP technique helps in rapid detection. Significance and impact of the study Pcb‐induced soft rot causes significant yield loss, decreased market value, damage in transit, storage and the market. Disease characterization and early identification aid in disease management and prevention in the field.

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Pectobacterium carotovorum subsp. carotovorum - the causal agent of broccoli soft rot in Serbia

Cited by 14 publications

References 17 publications

Amaranth cystatin inhibits the growth of broccoli (Brassica oleracea var. italica L.) pathogenic bacteria

Amaranth cystatin inhibits the growth of broccoli (Brassica oleracea var. italica L.) pathogenic bacteria

Вacterial rot of tomatoes when grown in a protected ground

Host range and virulence diversity of Pectobacterium carotovorum subsp. brasiliense strain RDKLR infecting radish in India, and development of a LAMP-based diagnostics

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