Pectolytic enzymes produced by Pseudomonas syringae pv. glycinea

Magro, P.

doi:10.1016/0378-1097(94)90162-7

Cited by 8 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Enzymes that are active at low temperatures are especially desirable for such degradation processes and would enable low energy treatment. Cold‐adapted PL producers described so far are predominantly bacterial representatives of a number of genera (Magro et al. 1994; Laurent et al .…”

Section: Introductionmentioning

confidence: 99%

Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida

Margesin¹,

Fauster²,

Fonteyne

2005

Lett Appl Microbiol

View full text Add to dashboard Cite

Aims: This study was conducted to determine optimal conditions for pectate lyase (PL) production by two psychrophilic yeast strains and to compare the properties of the cold-active enzymes using mesophilic PL as reference enzyme. Methods and Results: Two psychrophilic yeasts isolated from remote geographical locations (European Alps, north Siberia) produced extracellular cold-active PL. Both strains were identified as Mrakia frigida by analysis of ITS and large subunit (LSU) rRNA sequences. Maximum enzyme production occurred at a cultivation temperature of 1 or 5°C. The apparent optimum for enzyme activity was observed at 30°C and pH 8AE5-9. The enzymes were thermolabile, but were resistant to repeated freezing and thawing. Conclusion: We describe for the first time alkaline PL-producing representatives of the yeast species M. frigida. The two strains produce cold-active PL with similar properties, but have a different enzyme production pattern. Significance and Impact of the Study: The enzymes described in this study could be useful for a wide range of applications, such as low-temperature pretreatment of wastewater containing pectic substances.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida

Margesin¹,

Fauster²,

Fonteyne

2005

Lett Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…lycopersici (Jones et al, 1972;Lafitte et al, 1984;Powell et al, 2000). These proteins also have been found in the secretome of Arabidopsis roots when the plant is infected with P. syringae (Magro et al, 1994;De-la-Peña et al, 2008), which indicates that PGIPs participate in defense response signaling in the rhizosphere during bacterial attack. Therefore, PGIPs could be exploited as a strategy for protecting crops against PG-producing pathogens.…”

Section: Pgsmentioning

confidence: 83%

“…PGs are one of the factors responsible for causing severe diseases in many plants (Collmer and Keen, 1986;Magro et al, 1994;Shieh et al, 1997;Aysan et al, 2003). For instance, Aspergillus flavus, a saprophytic fungus responsible for important losses in maize, peanut (Arachis hypogaea), and cotton (Lillehoj et al, 1974;Shieh et al, 1997;Asis et al, 2005), produces a PG, P2c, which causes intercarpellary membrane damage due to the loss of pectin integrity, thus helping the spread and invasion of the fungus (Shieh et al, 1997).…”

Section: Pgsmentioning

confidence: 99%

Biotic Interactions in the Rhizosphere: A Diverse Cooperative Enterprise for Plant Productivity

De‐la‐Peña

Loyola‐Vargas

2014

PLANT PHYSIOLOGY

View full text Add to dashboard Cite

Microbes and plants have evolved biochemical mechanisms to communicate with each other. The molecules responsible for such communication are secreted during beneficial or harmful interactions. Hundreds of these molecules secreted into the rhizosphere have been identified, and their functions are being studied in order to understand the mechanisms of interaction and communication among the different members of the rhizosphere community. The importance of root and microbe secretion to the underground habitat in improving crop productivity is increasingly recognized, with the discovery and characterization of new secreting compounds found in the rhizosphere. Different omic approaches, such as genomics, transcriptomics, proteomics, and metabolomics, have expanded our understanding of the first signals between microbes and plants. In this review, we highlight the more recent discoveries related to molecules secreted into the rhizosphere and how they affect plant productivity, either negatively or positively. In addition, we include a survey of novel approaches to studying the rhizosphere and emerging opportunities to direct future studies.

show abstract

“…These are comparable with those of Bacillus sp. KSM-P15, having optimal activity around pH 10.5 and 50–55°C [18], and superior to several alkaline pectinases reported by Nasuno and Starr [19], Davé and Vaughn [20], and Magro et al [21], exhibiting the maximum activity at pH 8.5 and 45°C, and pH 8.0–8.5 and 60°C, pH 8.0 and 30–40°C, respectively. The PGL was stable at pH 7.0–10.5 and 30–50°C, and at least 81% of its maximum activity was retained within 8 h. These also showed that it was suitable for degumming ramie.…”

Section: Discussionmentioning

confidence: 99%

An Effective Degumming Enzyme fromBacillussp. Y1 and Synergistic Action of Hydrogen Peroxide and Protease on Enzymatic Degumming of Ramie Fibers

Guo

Zou

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Enzymatic degumming, as an alternative to chemical processing, has attracted wide attention. However, to date, little information about other enzyme components with effective degumming except pectinase has been reported, and there is no report about the effect of bleaching agent (H2O2) on enzymatic degumming and combining enzymatic degumming and H2O2 bleaching process. In this study, we found that the crude enzyme of wild-type Bacillus sp. Y1 had a powerful and fast degumming ability. Its PGL activity was the highest at pH 9.6–10.0 and 60°C and stable at pH 7–10.5 and 30–50°C, having a wide scope of pH and temperature. Its PGL also had a high H2O2 tolerance, and the gum loss and brightness of fibers could be significantly improved when H2O2 was added into it for degumming. The synergistic action was also found between it and H2O2 on the degumming and bleaching of ramie fibers. All showed that it was very suitable for a joint process of enzymatic degumming and H2O2 bleaching. It also contained more proteins compared with a control pectinase, and its high protease content was further substantiated as a factor for effective degumming. Protease and pectinase also had a synergistic action on degumming.

show abstract

Pectolytic enzymes produced by Pseudomonas syringae pv. glycinea

Cited by 8 publications

References 23 publications

Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida

Characterization of cold-active pectate lyases from psychrophilic Mrakia frigida

Biotic Interactions in the Rhizosphere: A Diverse Cooperative Enterprise for Plant Productivity

An Effective Degumming Enzyme fromBacillussp. Y1 and Synergistic Action of Hydrogen Peroxide and Protease on Enzymatic Degumming of Ramie Fibers

Contact Info

Product

Resources

About