Peculiar Ca2+ Homeostasis, ER Stress, Autophagy, and TG2 Modulation in Celiac Disease Patient-Derived Cells

Sposito, Silvia; Secondo, Agnese; Romanelli, Antonio Massimiliano; Montefusco, Antonio; Nanayakkara, Merlin; Auricchio, Salvatore; Barone, Maria Vittoria; Caputo, Ivana; Paolella, Gaetana

doi:10.3390/ijms24021495

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…Tg is an irreversible endoplasmic reticulum Ca 2+ -ATPase binder, which can induce endoplasmic reticulum protein folding disorder, leading to the accumulation of unprocessed proteins in the endoplasmic reticulum. This causes dysfunction of the endoplasmic reticulum and activation of SOCE, which is typically regarded as an agonist of endoplasmic reticulum stress (34). STIM1 is one of the key proteins of SOCE.…”

Section: Discussionmentioning

confidence: 99%

3,3'-Diindolylmethane inhibits the proliferation of esophageal squamous cell carcinoma cells via downregulation of STIM1

Xiong,

Tang,

et al. 2024

Oncol Lett

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3,3'-Diindolylmethane (DIM) is a natural phytochemical derived from cruciferous plants that has inhibitory effects on a wide range of tumor cells; however, its relevant effects on esophageal cancer cells have been poorly studied. Therefore, in the present study, a pharmacology network approach was used to predict the possible core targets of DIM acting on esophageal cancer. Subsequently, using in vitro experiments, TE-1 human esophageal cancer cells were treated with different concentrations of DIM (0, 40, 60 and 80 µM) for 24 h. Changes in cell activity were detected by Cell Counting Kit-8 assay, and changes in the expression levels of stromal interaction molecule 1 (STIM1) and apoptosis-related proteins, B-cell lymphoma-2 (Bcl-2) and Bax, were assessed by western blotting, followed by the upregulation of STIM1 by thapsigargin (Tg). Network pharmacology analysis showed that there were 39 potential core targets of DIM in esophageal cancer. The results of the in vitro experiments showed that DIM could inhibit the viability of esophageal cancer cells, downregulate the expression of STIM1 and Bcl-2 proteins and upregulate the expression of Bax protein, all in a concentration-dependent manner. The results also demonstrated that toxic carotenoids were agonist against STIM1 protein and upregulated STIM1 and Bax protein expression. After agonizing STIM1 protein expression using Tg, DIM was able to counteract the expression trend of STIM1, Bcl-2 and Bax protein in TE-1 cells. In summary, DIM induced apoptosis and inhibited the viability of esophageal cancer cells by downregulating the expression of STIM1 protein; therefore, the natural phytochemical, DIM, may be a potential substance for the early prevention and treatment of esophageal cancer cells.

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Section: Discussionmentioning

confidence: 99%

3,3'-Diindolylmethane inhibits the proliferation of esophageal squamous cell carcinoma cells via downregulation of STIM1

Xiong,

Tang,

et al. 2024

Oncol Lett

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“…There is still much to learn about several phenotypes of CD such as seronegative, slow-responding, and potential (limited lesions). With the development of substitute and complementary therapies, patients who cannot adhere to a gluten-free diet have cause for optimism [76].…”

Section: Management Of CDmentioning

confidence: 99%

Psychiatric and Neurological Manifestations of Celiac Disease in Adults

Alkhiari¹

2023

Cureus

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Celiac disease (CD), a chronic inflammatory disorder of the intestines, affects 0.7% to 1.4% of the world's population. CD causes diarrhea, abdominal discomfort, bloating, flatulence, and, in rare cases, constipation in the digestive tract. Since the identification of gluten as the disease-causing antigen, CD patients have been treated with a gluten-free diet, which is advantageous but has limitations for certain patient groups. CD is associated with mood disorders, such as manic-depressive disease, schizophrenia, and bipolar disorder, as well as other disorders such as depression and anxiety. The relationship between CD and psychological issues is not entirely understood. Here, we look at the most recent psychiatric data as they pertain to CD, as well as the relevant psychiatric manifestations that have been associated with this condition. Clinicians should examine mental health factors when a CD diagnosis is established. More research is needed to understand the pathophysiology of CD's psychiatric manifestations.

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Peculiar Ca2+ Homeostasis, ER Stress, Autophagy, and TG2 Modulation in Celiac Disease Patient-Derived Cells

Cited by 2 publications

References 40 publications

3,3'-Diindolylmethane inhibits the proliferation of esophageal squamous cell carcinoma cells via downregulation of STIM1

3,3'-Diindolylmethane inhibits the proliferation of esophageal squamous cell carcinoma cells via downregulation of STIM1

Psychiatric and Neurological Manifestations of Celiac Disease in Adults

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