“…Increasing the lipid content in the diet of cattle is known to mitigate greenhouse gas emissions and the supplementing feed with moderate amounts of oil reduces methane production by ruminants (Bayat et al, 2018;Singer et al, 2018). Limited research is available on profiling fatty acids in sainfoin (seed and vegetative tissue) (Tarasenko et al, 2015;Wijekoon et al, 2020), and further research is needed full characterization of the lipophilic fraction. The lipid fraction of fenugreek seeds revealed that oleic acid (C18:1), linoleic acid (C18:2), and α-linolenic acid (C18:3) are the main fatty acids present, but myristic acid (C14:0), pentadecylic acid (C15:0), palmitic acid (C16:0), margaric acid (C17:0), stearic acid (C18:0), arachidic acid (C20:0), eicosenoic acid (C20:1), and behenic acid (C22:0) were present in lower quantities (Chatterjee, Variyar, & Sharma, 2010, Bienkowski, Zuk-Golaszewska, Kaliniewicz, & Golaszewski, 2017Rathore et al, 2017).…”