Peculiarities of digestive function of proteinases in invertebrates—Inhabitants of cold seas

Mukhin, V. A.; Smirnova, E. B.; Novikov, V. Yu.

doi:10.1134/s0022093007050039

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Whilst lipids were mobilized faster than proteins in O. mimus, proteins were mobilized faster than lipids in O. maya. This finding suggests that the mobilization of lipids in temperate octopuses is different from the metabolic process that occurs in tropical octopuses (Mukhin et al 2007). The digestive enzyme activity in the GJ (mainly acidic proteases) and DG was greater in O. mimus than in O. maya.…”

Section: Discussionmentioning

confidence: 49%

Timing of digestion, absorption and assimilation in octopus species from tropical (Octopus maya) and subtropical-temperate (O. mimus) ecosystems

Linares¹,

Caamal‐Monsreal²,

Paz³

et al. 2015

Aquat. Biol.

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Section: Discussionmentioning

confidence: 49%

Timing of digestion, absorption and assimilation in octopus species from tropical (Octopus maya) and subtropical-temperate (O. mimus) ecosystems

Linares¹,

Caamal‐Monsreal²,

Paz³

et al. 2015

Aquat. Biol.

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“…From this figure it is evident that although the general process is similar between species, there are differences in the timing of the process and the form in which DG reserves are used. In O. mimus , lipids were mobilized faster than proteins while in O. maya an inverse relationship between proteins and lipids was observed, suggesting that mobilization of lipids could be a priority in temperate octopus species (Mukhin et al, 2007 ). Also, it was observed that in O. mimus the acidic enzyme activity in the digestive tract was greater than in O. maya , suggesting that through modulation by low temperatures O. mimus could require more enzymes to digest the meal.…”

Section: Octopus Maya and O Mimus Digestmentioning

confidence: 99%

Digestive Physiology of Octopus maya and O. mimus: Temporality of Digestion and Assimilation Processes

et al. 2017

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Digestive physiology is one of the bottlenecks of octopus aquaculture. Although, there are successful experimentally formulated feeds, knowledge of the digestive physiology of cephalopods is fragmented, and focused mainly on Octopus vulgaris. Considering that the digestive physiology could vary in tropical and sub-tropical species through temperature modulations of the digestive dynamics and nutritional requirements of different organisms, the present review was focused on the digestive physiology timing of Octopus maya and Octopus mimus, two promising aquaculture species living in tropical (22–30°C) and sub-tropical (15–24°C) ecosystems, respectively. We provide a detailed description of how soluble and complex nutrients are digested, absorbed, and assimilated in these species, describing the digestive process and providing insight into how the environment can modulate the digestion and final use of nutrients for these and presumably other octopus species. To date, research on these octopus species has demonstrated that soluble protein and other nutrients flow through the digestive tract to the digestive gland in a similar manner in both species. However, differences in the use of nutrients were noted: in O. mimus, lipids were mobilized faster than protein, while in O. maya, the inverse process was observed, suggesting that lipid mobilization in species that live in relatively colder environments occurs differently to those in tropical ecosystems. Those differences are related to the particular adaptations of animals to their habitat, and indicate that this knowledge is important when formulating feed for octopus species.

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“…The ability of midgut gland cells to secrete cathepsin D1 suggests that clawed lobsters follow a strategy to attain extracellular enzymes with originally intracellular enzymes and, thus, to digest food efficiently under conditions found in their habitats, as observed by Mukhin et al (2007).…”

Section: General Features Of Cathepsinsmentioning

confidence: 99%

Aspartic Cathepsin D Endopeptidase Contributes to Extracellular Digestion in Clawed Lobsters Homarus americanus and Homarus gammarus

et al. 2010

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Acid digestive proteinases were studied in the gastric fluids of two species of clawed lobster (Homarus americanus and Homarus gammarus). An active protein was identified in both species as aspartic proteinase by specific inhibition with pepstatin A. It was confirmed as cathepsin D by mass mapping, N-terminal, and full-length cDNA sequencing. Both lobster species transcribed two cathepsin D mRNAs: cathepsin D1 and cathepsin D2. Cathepsin D1 mRNA was detected only in the midgut gland, suggesting its function as a digestive enzyme. Cathepsin D2 mRNA was found in the midgut gland, gonads, and muscle. The deduced amino acid sequence of cathepsin D1 and cathepsin D2 possesses two catalytic DTG active-site motifs, the hallmark of aspartic proteinases. The putatively active cathepsin D1 has a molecular mass of 36.4 kDa and a calculated pI of 4.14 and possesses three potential glycosylation sites. The sequences showed highest similarities with cathepsin D from insects but also with another crustacean cathepsin D. Cathepsin D1 transcripts were quantified during a starvation period using real-time qPCR. In H. americanus, 15 days of starvation did not cause significant changes, but subsequent feeding caused a 2.5-fold increase. In H. gammarus, starvation caused a 40% reduction in cathepsin D1 mRNA, and no effect was observed with subsequent feeding.

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Peculiarities of digestive function of proteinases in invertebrates—Inhabitants of cold seas

Cited by 9 publications

References 15 publications

Timing of digestion, absorption and assimilation in octopus species from tropical (Octopus maya) and subtropical-temperate (O. mimus) ecosystems

Timing of digestion, absorption and assimilation in octopus species from tropical (Octopus maya) and subtropical-temperate (O. mimus) ecosystems

Digestive Physiology of Octopus maya and O. mimus: Temporality of Digestion and Assimilation Processes

Aspartic Cathepsin D Endopeptidase Contributes to Extracellular Digestion in Clawed Lobsters Homarus americanus and Homarus gammarus

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