Peculiarities of Proliferation of Epidermal Cambial Cells in Mouse Skin

Abstract

The long axis of epidermal cambial cells determining the direction of their division is determined before the beginning of division and is oriented perpendicularly, but not parallel, to the basal membrane, as in other basal cells. As a result, only one of the two newly formed cells adheres to the basal membrane and at the expense of traction forces detaching one cell from the other and elastic force of the basal membrane is formed as a maternal cell and stays in the focus of multiplication. The other cell, turning around under the effect of traction forces perpendicularly to the maternal cell, cardinally changes the direction of its long axis and is polarized parallel to the basal membrane like other cells. This cell becomes the daughter cell, its shape with the "head" and "tail" allows its rapid migration into other rosettes for differentiation.

Interaction of cambial dermal cells (fibroblasts) and epidermis in morphofunctional area of mouse skin

“…These cells had hyperchromatic nucleus and poorly stained cytoplasm with projections like those of the fibroblasts; they transform through the stages analogous to those of epidermal CC (Fig. 1, a) [5]. Two daughter cells of a small cell are located one under another.…”

Peculiarities of Proliferation and Differentiation of Cambial and Daughter Cells of Epidermal-Dermal Morphofunctional Zone in Normal Epithelium and in Cancer

“…The long axis of the cambial cells is perpendicular to the basal membrane, but not parallel to it as in other cells. Therefore, after its division one cells (maternal) is still adjacent to the basal membrane, while the other (daughter) cell is located above it [9,10]. These cells remain in close contact, which blocks polarization of the maternal cell along its major axis, because it is mechanically squeezed between the daughter cell and basal membrane.…”