Peculiarities of RBC aggregation studied by double trap optical tweezers

Khokhlova, Maria D.; Lyubin, Evgeny V.; Zhdanov, A. G.; Rykova, S.Y.; Krasnova, Tatyana; Соколова, И. А.; Fedyanin, Andrey A.

doi:10.1117/12.854502

Cited by 4 publications

(4 citation statements)

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“…19 The use of optical tweezers was also proposed for qualitative analysis of red cell (dis)aggregation. 20,21 In this paper, quantitative determination of force parameters of normal and pathological RBC pair aggregation utilizing double-trap optical tweezers is performed. This experimental approach provides a method to probe RBC aggregation mechanisms between individual cells and can offer insights into the connection between two theories of RBC aggregation.…”

Section: Introductionmentioning

confidence: 99%

Normal and system lupus erythematosus red blood cell interactions studied by double trap optical tweezers: direct measurements of aggregation forces

et al. 2012

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Abstract. Direct measurements of aggregation forces in piconewton range between two red blood cells in pair rouleau are performed under physiological conditions using double trap optical tweezers. Aggregation and disaggregation properties of healthy and pathologic (system lupus erythematosis) blood samples are analyzed. Strong difference in aggregation speed and behavior is revealed using the offered method which is proposed to be a promising tool for SLE monitoring at single cell level.

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Section: Introductionmentioning

confidence: 99%

Normal and system lupus erythematosus red blood cell interactions studied by double trap optical tweezers: direct measurements of aggregation forces

et al. 2012

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“…Topics of RBC assessment using optical devices have been of immense interest, as they provide vital information regarding the microcirculatory parameters such as RBC aggregation forces using optical tweezers [357][358][359], and studies have pursued the assessment of aggregation dynamics [360]. One can actively implement this technology to assess biological parameters such as rheology and hemodynamics, although a few shortcomings related to effective measurements need reconsiderations [361].…”

Section: Optical Trappingmentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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“…Similarly, Khokhlova et al applied double-trap optical tweezers to determine an aggregation force for normal and system lupus erythematosus (SLE) RBCs. They found that the aggregation speed of normal RBCs was almost two times lower than that of SLE ones, which could be applied to monitor SLE and its response to drug therapies [124]. Meanwhile, Maklygin et al used double-beam optical tweezers to measure the interaction forces as 8.4 ± 1.1 pN between eight-RBC linear aggregates [125].…”

Section: Optical Aggregation Of Rbcsmentioning

confidence: 99%

Multifunctional manipulation of red blood cells using optical tweezers

Xie

Liu

2021

Journal of Biophotonics

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Serving as natural vehicles to deliver oxygen throughout the whole body, red blood cells (RBCs) have been regarded as important indicators for biomedical analysis and clinical diagnosis. Various diseases can be induced due to the dysfunction of RBCs. Hence, a flexible tool is required to perform precise manipulation and quantitative characterization of their physiological mechanisms and viscoelastic properties.Optical tweezers have emerged as potential candidates due to their noncontact manipulation and femtonewton-precision measurements. This review aimed to highlight the recent advances in the multifunctional manipulation of RBCs using optical tweezers, including controllable deformation, dynamic stretching, RBC aggregation, blood separation and Raman characterization. Further, great attentions have been focused on the precise assembly of functional biophotonics devices with trapped RBCs, and a brief overview was offered for the growing interests to manipulate RBCs in vivo.

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Peculiarities of RBC aggregation studied by double trap optical tweezers

Cited by 4 publications

References 0 publications

Normal and system lupus erythematosus red blood cell interactions studied by double trap optical tweezers: direct measurements of aggregation forces

Normal and system lupus erythematosus red blood cell interactions studied by double trap optical tweezers: direct measurements of aggregation forces

Advances in Microfluidics for Single Red Blood Cell Analysis

Multifunctional manipulation of red blood cells using optical tweezers

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