Peculiarities of somatic embryogenesis of long-term proliferating embryogenic cell lines of Larix sibirica in vitro

Tretyakova, I. N.; Park, Maria E.; Ivanitskaya, A. S.; Орешкова, Н. В.

doi:10.1134/s1021443716050137

Cited by 14 publications

(7 citation statements)

References 29 publications

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“…The method of individual conifer species microclonal propagation by somatic embryogenesis has already begun to be widely used in various countries of the world, primarily in France, Canada, Germany, Great Britain, Ireland, Scandinavian countries, China, in plantation forestry and in the implementation of MVF (Multi Variety Forest) programs (Park et al, 2016). MVF is defined as the use of a range of genetically tested woody species in a production forest seed orchard (Weng et al, 2011).…”

Section: Use Of the Methods Of Somatic Embryogenesis In Representativementioning

confidence: 99%

“…This technology, based on a set of mapping of quantitative traits loci, makes possible the prediction of the phenotype of an individual (Goddard, Hayes, 2007). Thus, genomic selection makes it possible to identify elite genotypes at a very early stage of development without phenotyping through field testing and, thereby, to reduce significantly the duration of variety testing within the framework of afforestation programs (Park et al, 2016).…”

Section: Use Of the Methods Of Somatic Embryogenesis In Representativementioning

confidence: 99%

“…Sometimes, indolebutyric acid at 1.0 mg/L (Tret'yakova et al, 2012), 5.0 mg/L of auxin transport inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB), 5.0 mg/L phloroglucinol (auxin synergist) (Kim, Moon, 2009) or silver nitrate (2.0-5.5 mg/L) (Saly et al, 2002;Song et al, 2018) are used together with abscisic acid to improve the process of maturation of somatic embryos. Improving the quality of somatic embryos, their germination and the formation of full-fledged plants is achieved by combining abscisic acid with activated carbon (0.5-10 g/L), which is introduced into the nutrient medium during the pre-maturation of somatic embryos (Harry et al, 1991;Qi et al, 2004;Umehara et al, 2004;Tretyakova et al, 2016). Considering that maturing somatic embryos should be exposed to water stress, similar to developing zygotic embryos in vivo, substances such as polyethylene glycol 3000-4000 at 4-10 %, sucrose at an increased concentration (3-8 %) or maltose (3 %), and gelling agents gelright or phytagel (0.3-0.4 %) are introduced into the nutrient medium in order to reduce the available water (Klimaszewska et al, 1997;Ma et al, 1998;Qi et al, 2004;Lu et al, 2005;Teyssier et al, 2011;Tret'yakova, Barsukova, 2012;Song et al, 2018).…”

Section: Composition Of Culture Media For Embryogenic Masses and Somamentioning

confidence: 99%

“…According to microsatellite analysis, embryogenic lines were characterized by weak allelomorphic variability (Tretyakova et al, 2017). In general, nonetheless, the capacity of somatic embryos from long-term maintained lines for maturation and germination decreased sooner or later (Tretyakova et al, 2016).…”

Section: Dependence Of Somatic Embryogenesis Potential Of Larch Treesmentioning

confidence: 99%

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Somatic embryogenesis in Larix: the state of art and perspectives

Шмаков

Konstantinov

2020

Vestn. VOGiS

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Clonal propagation of conifers using somatic embryogenesis is essential for the selection of tree species, and for the implementation of afforestation and reforestation. In combination with cryopreservation, somatic embryogenesis creates the basis for the development of economically valuable lines of clones and elite genotypes. The industrial use of such genetically verified clone lines in forestry can significantly increase forest productivity compared to any conventional methods for improving tree crops that are available. Larch is considered as one of the main conifer candidates for large-scale reforestation, not only due to the vastness of its habitat, but also due to the unique quality of its wood, rapid growth and high ecological plasticity. However, the vast majority of larch species are characterized by uneven yields and extremely low seed quality. In this regard, obtaining planting material for reforestation from larch seeds on seed plantations is not advisable, but can be successfully implemented in afforestation programs using somatic embryogenesis technologies. Research on the somatic embryogenesis of larch has been conducted for over 30 years, which allowed considerable experience in this field to be accumulated. To date, the conditions for the initiation and maintenance of embryogenic cultures, as well as for the formation and development of somatic embryos have been determined. Significant progress has been made in the study of both the factors affecting these processes and the molecular mechanisms that underlie the various stages of embryogenesis. Nevertheless, despite the successes achieved, knowledge available today on the somatic embryogenesis of representatives of the genus Larix is still not enough to develop technologies for producing valuable plant-breeding material in vitro. This review analyzes the current state of research on the problem of somatic embryogenesis of representatives of the genus Larix. Particular attention is paid to the choice of explants for somatic embryogenesis, the composition of the media for cultivation, the dependence of the potential of somatic embryogenesis on the duration of cultivation, and the genetic control of somatic embryogenesis.

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Section: Use Of the Methods Of Somatic Embryogenesis In Representativementioning

confidence: 99%

Section: Use Of the Methods Of Somatic Embryogenesis In Representativementioning

confidence: 99%

Section: Composition Of Culture Media For Embryogenic Masses and Somamentioning

confidence: 99%

Section: Dependence Of Somatic Embryogenesis Potential Of Larch Treesmentioning

confidence: 99%

See 2 more Smart Citations

Somatic embryogenesis in Larix: the state of art and perspectives

Шмаков

Konstantinov

2020

Vestn. VOGiS

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“…Callus was transferred to fresh nutrient medium every two weeks. Maturation and germination of somatic embryos were described by us earlier [15].…”

Section: Proliferation Of Embryonic Massmentioning

confidence: 99%

Reproduction and Genetic Stability of Long-Term Proliferative Embryogenic Cell Lines of Larix Sibirica In Vitro

Nt¹

2017

IJCSMB

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The study of quality, reproduction and somaclonal changes of long-term proliferative Embryogenic cultures of Larix sibirica in vitro was conducted. Produced cell lines of Larix sibirica were characterized by high Embryogenic productivity. Multiplication of somatic embryos was activated through the cleavage budding and disintegrating suspensor cells. Cultivated Embryogenic cultures did not change the ploidy for seven years. According to microsatellite analysis proliferating cell lines of Siberian larch was characterized by weak allelic variability, and four-year cloned seedlings were genetically stabled.

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