“…Electrical stimulation of the bladder was carried out with a constant current stimulator (World Precision Instruments DS8000, Sarasota, FL, USA) to simulate sensory afferents to PAG. Sensory and motor fibers are more effectively stimulated at longer (0.4 ms) and shorter (0.05–0.15 ms) pulse widths, respectively . After a 2‐week recovery period, electrical stimulation of the bladder in the stimulation group was carried out giving bipolar square wave pulses of 20 Hz with an intensity of 2 mA.…”