Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up

Atluri, Venkata Subba Rao; Gogulamudi, Venkateswara R; Singhi, Pratibha; Khandelwal, Niranjan; Parasa, Lakshmana Swamy; Malla, Nancy

doi:10.1155/2014/904046

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…The LLGP‐EITB, however, is technically complicated, requires sophisticated laboratory capacity and is not widely available. Despite previously published data pointing to inferior diagnostic performance , many investigators still use antibody detection by ELISA for diagnostic purposes . Commercial ELISA assays are offered by most diagnostic laboratories in the United States and Europe, and globally ELISA for antibody is the most commonly performed serologic assay for cysticercosis.…”

Section: Introductionmentioning

confidence: 99%

Low sensitivity and frequent cross‐reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Garcı́a

Castillo²,

Gonzáles

et al. 2017

Tropical Med Int Health

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Abstractobjective To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa â and Ridascreen â , for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentillectin purified glycoprotein antigens).methods Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa â and Ridascreen â . All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits.results Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%).conclusions The performance of Novalisa â and Ridascreen â was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

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Section: Introductionmentioning

confidence: 99%

Low sensitivity and frequent cross‐reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Garcı́a

Castillo²,

Gonzáles

et al. 2017

Tropical Med Int Health

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“…In the present investigation, samples revealing anti-cysticercus antibodies against ESA (30.76 %) were on the lower side compared to Sahu et al (2015) , who observed 45 % seropositivity among ocular CC cases. Antibody response to ESA in confirmed NCC patients has been observed to the tune of 88.2 % and 66.6 % among 34 and 36 cases from Pondicherry ( Sahu et al , 2009 ) and Chandigarh ( Atluri et al , 2014 ) of India, respectively. Molinari et al (2002) observed an anti-cysticercus antibody against ESA in the cerebrospinal fluid (CSF) of 24 (17 %) of patients out of 91 with a neurological disorder.…”

Section: Discussionmentioning

confidence: 99%

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Satyaprakash,

Khan,

Zade

et al. 2023

Helminthologia

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SummaryNeurocysticercosis (NCC), one of the most important neuroparasitic diseases in humans, is caused byCysticercus cellulosae, the metacestode stage of digenetic zoonotic cestodeTaenia solium. The present study aims at the detection of anti-cysticercus antibodies in the sera of epileptic patients (n=26) visiting a tertiary care hospital in Nagpur, Maharashtra state, India, by an in-house developed indirect IgG-ELISA and enzyme-linked immunoelectro transfer blot (EITB) assay using different antigens (namely, Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA), Excretory-Secretory Antigen (ESA) and Membrane-Body Antigen (MBA)) prepared fromT. soliummetacestodes to find out the status of NCC. An attempt has also been made for molecular detection of NCC from blood samples of those patients by Polymerase Chain Reaction (PCR) assay targeted atlarge subunit rRNAgene ofT. solium. The IgG ELISA level of anti-cysticercus antibodies against WCA, CFA, SA, ESA and MBA antigens were as follows: 19.23 %, 23.07 %, 38.46 %, 30.76 % and 15.38 %. The seroreactivity to CFA, SA and ESA was found in equal proportions in patients with ring-enhancing lesions. In the EITB assay, the lower and medium molecular weight protein bands of SA and ESA were immunodominant compared to the higher WCA and CFA peptides. PCR positivity could be observed in 34.6 % (9/26) of the patients under study. It is the first report of detecting NCC among epileptic patients of the Nagpur region of Maharashtra state in India using serological and molecular tools.

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“…Una localización que puede ser diagnosticada durante la exploración física es la ocular, ya que, al observar el fondo de ojo, se puede ver el parásito en el espacio subretiniano o en el humor vítreo. Esta presentación puede poner en riesgo la visión, por la inflamación y degeneración de los quistes o el desprendimiento de la retina (14,16).…”

Section: Discussionunclassified

Neurocisticercosis en preescolares: presentación de caso

2016

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Introducción: La neurocisticercosis (ncc) es la infección parasitaria más común del sistema nervioso central causada por la larva de la Taenia solium. Esta patología es endémica en los países en vías de desarrollo y presenta su mayor incidencia en pacientes escolares y adolescentes de la población pediátrica. Muestra una diversidad clínica y hallazgos patológicos, en general, se presenta con formas intraparenquimatosas asociadas con convulsiones o formas extraparenquimatosas relacionadas con hipertensión endocraneana. La sospecha clínica y epidemiológica de la enfermedad obliga a solicitar estudios de tomografía axial computarizada (tac) y/o resonancia magnética, además de los estudios serológicos específicos. Presentación de caso: se describe un caso clínico de una niña preescolar con neurocisticercosis sucedido en el Hospital Infantil Napoleón Franco Pareja (hinfp) en Cartagena de Indias, Colombia, el cual se caracteriza por presentar un cuadro clínico que debuta con crisis tónicas en extremidades, por lo que se solicita estudio de tac cerebral, que muestra imágenes de encefalitis compatible con cistercosis cerebral, se decide hospitalizar iniciándose manejo cesticida, anticonvulsivante y antiinflamatorio. Conclusión: Los hallazgos imagenológicos y la evolución clínica de la neurocisticercosis en la infancia están asociados con el estadio de la enfermedad y la respuesta inmunológica del huésped.

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Pediatric Neurocysticercosis: Usefulness of Antibody Response in Cysticidal Treatment Follow-Up

Cited by 7 publications

References 30 publications

Low sensitivity and frequent cross‐reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Low sensitivity and frequent cross‐reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Neurocisticercosis en preescolares: presentación de caso

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