Pediatric peripheral blood progenitor cell collection: haemonetics MCS 3P versus COBE Spectra versus Fresenius AS104

Bambi, Franco; Faulkner, Lawrence; Azzari, Chiara; Gelli, Anna Maria Grazia; Tamburini, Anna; Tintori, Veronica; Lippi, Alma; Tucci, Fábio C.; Bernini, Gabriella; Genovese, Federica

doi:10.1046/j.1537-2995.1998.38198141501.x

Cited by 28 publications

(27 citation statements)

References 6 publications

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“…Although there was a thrombocyte loss after PPC collection, median platelet counts were equal before and after apheresis due to the described thrombocyte recirculation and additional homologous platelet transfusion in two children with platelet counts below 30,000/µl prior to apheresis. Our data were confirmed by Bambi et al [13] who found that postapheresis platelet counts dropped, at least in pediatric PPC collections with the Fresenius machine in comparison with the Haemonetics MCS 3p and COBE Spectra devices. Hematological recovery after autologous PPC transplantation was achieved in our 6 transplanted patients, and the median days of WBC recovery were similar to those reported by other authors [14,15].…”

Section: Discussionsupporting

confidence: 89%

Colledction of Peripheral Progenitor Cells in Pediatric Patients with Reduced Volume Technique

Moog¹,

Basu²,

Fuchs³

et al. 1999

Transfus Med Hemother

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Background: When peripheral progenitor cells (PPC) are harvested in children the special situation of their circulatory system has to be taken into account. Extracorporeal blood volume and product volume should be low to avoid side effects. Setting: Hemapheresis unit and pediatric oncologic ward of a University Clinic. Patients: 12 children with neuroblastoma, Ewing sarcoma, unclassified malignant bone tumor, medulloblastoma, pheochromocytoma, rhabdomyosarcoma and Wilms tumor. Material and Methods: The children underwent 24 PPC collections with a continuous flow system and a reduced volume programme. PPC were mobilized by chemotherapy and G-CSF application. Blood counts and CD34 antigen-expressing cells were determined before apheresis and in the PPC product. PPC were frozen at controlled rates and stored in liquid nitrogen until they were used for autologous transplantation. Results: Except for anticoagulant-induced venous irritation, no adverse side effects were observed. The median product volume was 59 ml (range 34–119 ml) and the yield of CD34 antigen-expressing cells was 1.9 × 10⁶/kg body weight (0.1 × 10⁶– 20.4 × 10⁶/kg). The platelet contamination of the products was 0.25 × 10¹¹(0.05 × 10¹¹–1.43 × 10¹¹). Median hematopoietic reconstitution for platelets was achieved on day 27 after transplantation and for leukocytes on day 10.5, respectively. Conclusion: PPC can be easily collected in children with the reduced product volume technique. The system is adopted to the small blood volume of infants so that no circulatory side effects were observed. The reduced volume technique helps lessening the quantity of dimethylsulfoxide needed for cryopreservation as well as reducing storage space. Platelet contamination of the product was low due to retransfusion of platelet-rich plasma to the patients prior to PPC harvesting.

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Section: Discussionsupporting

confidence: 89%

Colledction of Peripheral Progenitor Cells in Pediatric Patients with Reduced Volume Technique

Moog¹,

Basu²,

Fuchs³

et al. 1999

Transfus Med Hemother

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“…The collection of PBSC can be performed on various cell separator devices operating with semiautomated or automated apheresis programs [9, 10, 11]. The aim of the present study was to evaluate collection yields of conventional-volume COBE Spectra AutoPBSC leukaphereses and to demonstrate clinical significance of collected PBSC following HDCT.…”

Section: Discussionmentioning

confidence: 99%

“…Other reports described CD34-CE between 48 and 87% at preapheresis peripheral blood CD34+ cell counts of approximately 30/µl, using the cell separators Fresenius AS104, Haemonetics MCS-3P, COBE Spectra (protocol 4.7) and/or Fenwal CS3000 [9, 10, 11, 19, 20, 21]. Some authors reported better CD34-CE at a preapheresis level of circulating CD34+ cells <50/µl [13, 22].…”

Section: Discussionmentioning

confidence: 99%

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

et al. 2000

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Background: A convenient, effective and safe peripheral blood stem cell (PBSC) apheresis procedure is desirable to cope with the increasing requirements for PBSC collections. We performed PBSC harvesting with the novel COBE Spectra AutoPBSC^TM system using the default software configuration recommended by the manufacturer. We analyzed collection parameters and clinical efficiency of harvested autografts following high-dose chemotherapy (HDCT). Patients and Methods: Eighty-one patients underwent 102 harvests after standard chemotherapy plus filgrastim (5–10 μg/kg/day) to obtain a target of ≥2.5 × 10⁶ CD34+ cells/kg for autologous blood stem cell transplantation. Conventional-volume leukaphereses (median: 11 liters) were performed using the manufacturer’s standard software default regarding inlet flow, harvest/chase volume (3/7 ml) and number of collection cycles. The ratio of ACD-A to whole blood was initially set at 1:12 (56 collections), later at 1:10 (46 aphereses). Results: With respect to preapheresis counts of 93 (9–876) CD34+ cells/μl, 69 patients (85.2%) achieved ≥2.5 × 10⁶ CD34+ cells/kg by the first apheresis. PBSC products contained medians of 5.0 × 10⁶ (0.7–77.3) CD34+ cells/kg and 13.8 × 10⁴ (2.3–105.0) CFU-GM/kg. A preapheresis count of ≥40 CD34+ cells/μl predicted a single-apheresis yield of ≥2.5 × 10⁶ CD34+ cells/kg. Apheresis products showed a high mononuclear cell (MNC) purity of ≥89%. The median overall collection efficiency of CD34+ cells (CD34-CE) was 42.6% (12.2–87.4). The CD34-CE decreased significantly with increasing numbers of circulating CD34+ cells: 52.5% at CD34+ cells <40/μl versus 41.0% at CD34+ cells ≥40/μl (p ≤ 0.002). Coagulation problems occurred in 3 of 56 procedures at an ACD-A to whole blood ratio of 1:12, but in no case at 1:10. Patients who underwent HDCT and autologous blood stem cell transplantation (n = 40) engrafted in a median time of 9 (8–13) days for absolute neutrophil counts >0.5 × 10³/μl, 10 (8–13) days for WBC >1.0 × 10³/μl and 11 (8–17) days for platelets >20 × 10³/μl. Conclusions: As a result of efficient PBSC mobilization, a single conventional-volume leukapheresis with the COBE Spectra AutoPBSC system resulted in hematopoietic autografts with ≥2.5 × 10⁶ CD34+ cells/kg in 85% of patients. Following the standard PBSC apheresis recommendations of the manufacturer, the AutoPBSC system assures PBSC products with a high MNC purity and a moderate CD34-CE that declines significantly at increasing levels of circulating CD34+ cells. Leukaphereses performed at an ACD-A to whole blood ratio of 1:10 should run without coagulation problems.

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“…The topic of final product volumes using different cell separator systems has been discussed controversially in previous studies [5,9,11,16]. An apheresis procedure resulting in a small product volume facilitates the subsequent processing of the product [1,4,5,7,8,17]. In this respect, the Fresenius system proves to be preferable to the COBE Spectra system.…”

Section: Discussionmentioning

confidence: 99%

“…However, in the recent years, different clinical regimens for transplantation have been developed, taking advantage of improved approaches to collect peripheral blood stem cells (PBSC) [1][2][3]. PBSC are used for autologous transplantation, and with increasing tendency also for allogeneic transplantation [3][4][5][6][7][8]. Different types of cell separators have been introduced for obtaining PBSC.…”

Section: Introductionmentioning

confidence: 99%

Prospective Study on the Comparison of Two Apheresis Systems for Collecting Peripheral Blood Stem Cells

Herrmann¹,

Cassens²,

Krakowitzky³

et al. 2001

Transfus Med Hemother

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Background: In recent years, different clinical regimens of blood stem cell transplantation have been evaluated using peripheral blood stem cells (PBSC). Several types of cell separators were introduced for an efficient collection of PBSC. Material and Methods: In a prospective study at the Institute of Transfusion Medicine at the Münster University Hospital, two different cell separators were compared (COBE Spectra apheresis system, software version 5.1 / Fresenius AS.TEC 205 cell separator, software version 2.0.0) with regard to the collection of autologous peripheral blood stem cells. Patients: 7 patients (1 female, 6 male) with acute myeloid leukaemia (n = 2), multiple myeloma (n = 2), non-Hodgkin’s lymphoma (n = 2), testicular tumour (n = 1). Parameters of the investigation: efficiency of stem cell collection (yield/time), reduction of platelet counts and haemoglobin values during the apheresis process, product volume and ACD consumption. Results: The different parameters were evaluated at the basis of an equivalent length of time of each of the apheresis procedures. The volume of blood processed per time unit was significantly higher in the case of using the COBE Spectra apheresis system, and so was the ACD consumption. Upon comparison of yields of PBSC (CD34+ cells/kg body weight), we observed a trend in favour of a higher efficiency in the case of the COBE Spectra apheresis system. However, this trend did not reach statistical significance. The product volume was significantly lower in case of collecting PBSC with the Fresenius cell separator system than in case of using the COBE Spectra cell separator system. Carrying out the apheresis with the Fresenius cell separator system resulted in a diminished reduction of platelet counts and haemoglobin values in the course of the apheresis procedure, when compared to the COBE Spectra system. Conclusion: Both cell separators are well suitable for an efficient PBSC collection. Differences in the blood volume processed per time unit, the consumption of ACD, the yield of PBSC per time unit and the reduction of platelet counts and haemoglobin values allow for the individual selection of the optimal apheresis system guided by the clinical condition of each patient.

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Pediatric peripheral blood progenitor cell collection: haemonetics MCS 3P versus COBE Spectra versus Fresenius AS104

Abstract: All machines seem to have comparable PBPC extraction efficiency, but the AS104 seems to give the component with the greatest PBPC enrichment. This feature might be relevant for further ex vivo cell processing (CD34+ cell selection, expansion, and so on).

Cited by 28 publications

References 6 publications

Colledction of Peripheral Progenitor Cells in Pediatric Patients with Reduced Volume Technique

Colledction of Peripheral Progenitor Cells in Pediatric Patients with Reduced Volume Technique

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

Prospective Study on the Comparison of Two Apheresis Systems for Collecting Peripheral Blood Stem Cells

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