Objective: We aimed to evaluate the impact of a low-dose whole-body computed tomography (WBCT) protocol on radiation doses in pediatric major trauma patients. Methods: Retrospective cohort study of pediatric trauma patients (<16 years) at a national level 1 pediatric trauma center (PTC) over a 6-year period prior and post introduction of a low-dose WBCT protocol (2014–2019). Demographic data, patient characteristics, CT device, and exposure information including scan range, dose-length product (DLP), and volume computed tomography dose index (CTDIvol) were collected. Effective dose (ED) and exposure parameters were compared before and after protocol introduction. Results: Forty-eight patients underwent WBCT during the study period. Prior to introduction of the low-dose protocol (n =18), the ED was 20.6 mSv (median 20.1 ± 5.3 mSv [range 12.5-30.7]). After introduction of the low-dose WBCT protocol (n = 30), mean ED was 4.8 mSv (median 2.6 ± 5.0 [range: 0.8–19.1]). This resulted in a reduction of 77% in mean ED (p value <0.001). Conclusions: Significant radiation dose reduction of 77% can be achieved with low-dose WBCT protocols in pediatric trauma centers