Pedicle frozen autograft for limb sparing surgery in a dog with femoral osteosarcoma

Abstract

Osteosarcoma (OSA) is the most common bone tumor diagnosed in dogs and

Three cases of dogs with osteosarcoma of the forelimb treated with liquid nitrogen for limb-sparing surgery using autologous bone

“…The long-term prognosis is also good, with reported postoperative complications such as fractures (19.4%), deep infection (11.1%), and local soft tissue recurrence (11.1%), with no recurrence detected within the liquid nitrogen-treated autogenous bone [ 4 ]. Limited reports in the veterinary field also indicated good postoperative mandibular and gait function improvements in cases where reconstruction methods utilizing liquid nitrogen-treated autologous bone were applied [ 1 , 3 , 12 , 16 ]. Although none of the aforementioned complications were observed, exudate was observed postoperatively in Case 1 and screw breakage in Case 2.…”

“…In Tsuchiya et al .’s protocol, the extracted affected bone is treated with liquid nitrogen at −196°C for 20 min, left at a room temperature of 20°C for 15 min, and incubated in a saline solution at 30°C for 10 min, for a total duration of 45 min [ 21 ]. In veterinary medicine, tumor bone has been reconstructed using liquid nitrogen cryoprocessed bone tissue in the scapula in cats [ 1 ] and the mandible [ 12 ], femur [ 3 ], and tibia [ 16 ] in dogs. However, there are few reports of reconstructive surgery using liquid nitrogen-treated autologous bone in the veterinary field.…”

Osteosarcoma in Dogs: Diagnosis and Treatment - A Literature Review

“…These are: Chen at. Al [2][3][4][5][7][8][9][10][11][12][13][14]. The animals described in this review, the animals were middle aged to elderly and medium to large size, except for one case of a Maltese dog reported by Teixeira et al [18].…”

“…The study by Ferrigno et al [8] still includes abdominal ultrasound. The dentification of metastases and other diseases is fundamental to planning the treatment and, consequently, obtaining better prognosis [13,14,21,23] Regarding the treatment, Ferrigno et al [8] and Gôuvea et al [2], reported that limb preservation surgery, considered to be the best choice of treatment, as well as emphasized by Ziliotto et al [7], when combined with adjuvant chemotherapy provided a longer survival. In addition, the study by Ziliotto et al [7] has improved the surgical technique focused on the preservation of the bone implant in glycerin, which proved to be efficient for this purpose.…”

Efficacy of frozen autograft treated with liquid nitrogen in limb-sparing surgery in feline scapular osteosarcoma: A case report

“…Additionally, the incidence of complications, such as postoperative infection and construct failure, is high [5, 7]. To the best of our knowledge, there have been only two reports of frozen autograft reconstruction in dogs [2, 8], but none in cats.…”