Pediococcus acidilactici isolated from traditional cheese as a potential probiotic with cytotoxic activity against doxorubicin-resistant MCF-7 cells

Khaleghi, Moj; Khorrami, Sadegh; Jafarinasab, Tayebeh

doi:10.1007/s13205-023-03597-w

Cited by 4 publications

(1 citation statement)

References 57 publications

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“…Several Pediococcus strains have been investigated based on these criteria, isolated from various sources including dry-cured meat (104), fruit processing residues (105), human sources such as milk (106) and ileal epithelium (107), fermented food like cheese (42,108), salted and fermented sea-food (109), fermented cucumber (110), meat product (11), and fermented cereal-dairy product (12), and animal sources such as gastrointestinal tract of broilers (111) and cow manure (112). Among these strains, P. pentosaceus and P. acidilactici are particularly notable due to the presence of multiple strains identified with probiotic potential.…”

Section: Pediococcus Biocontrol Potentialmentioning

confidence: 99%

Genomic characterization and antimicrobial activity of Pediococcus pentosaceus ST65ACC

Oliveira

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The bacteriocinogenic strain Pediococcus pentosaceus ST65ACC was previously isolated from Brazilian artisan cheese (BAC) and showed strong bactericidal activity against Listeria monocytogenes. This strain also showed beneficial properties, such as the ability to survive gastrointestinal conditions and to aggregate with L. monocytogenes, indicating it as a potential probiotic candidate. The present study aimed to genomically characterize the P. pentosaceus ST65ACC strain through whole-genome sequencing (WGS), analyzing its technological and beneficial properties, bacteriocin clusters, and safety profiles, and also evaluating its antimicrobial activity against L. monocytogenes Scott A in different culture media. The genome of P. pentosaceus ST65ACC comprises 1,933,194 bp witha GC content of 37.00 %, and contains1,950 protein coding sequences (CDSs) with 62 RNA genes (6 rRNA, 55 tRNA, 1 tmRNA). Genomic analysis revealed the presence of operons that encode the bacteriocins pediocin PA-1/AcH and penocin-A, and also identified the presence of genes related to beneficial properties, such as stress adaptation genes and adhesion genes. Regarding the strain safety profiles, genes encoding biogenic amines and virulence genes were not detected. Genes related to antibiotic resistance were identified in the genome, but not in prophage regions, and no plasmids were detected, thus presenting a low risk of transferring to other bacteria. The results obtained fromthe interaction of P. pentosaceus ST65ACC and L. monocytogenes Scott A in MRS broth, BHI broth, milk, and meat broth for 96 h showed that the antimicrobial activity of the strain was more effective in MRS broth. L. monocytogenes was inhibited toun detectable levels by the strain in MRS broth during the 96 h of analysis, with high levels of bacteriocin production (3,200 – 12,800 AU/ml) being identified. However, lower inhibitory activities were recorded in BHI, milk, and meat broth, with low or no production of bacteriocins at the times analyzed. Thus, verifying that the composition of the media can interfere with the production/activity of bacteriocins and, consequently, with the antagonistic activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The results obtained through genomic characterization confirmed the beneficial potential and safety of P. pentosaceus ST65ACC, indicating that this strain can be considered suitable for application in food biopreservation, as well as a promising probiotic candidate. Keywords: Pediococcus. Biopreservation. Bacteriocins. Pediocin. Genomic analysis.

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Section: Pediococcus Biocontrol Potentialmentioning

confidence: 99%

Genomic characterization and antimicrobial activity of Pediococcus pentosaceus ST65ACC

Oliveira

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Hepatoprotective effect of astaxanthin against cholestasis liver fibrosis induced by bile duct ligation in adult Wistar rats

Laderian,

Ghasemi,

Mortazavi

et al. 2024

J Biochem & Molecular Tox

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In this study, we evaluated the hepatoprotective effects of astaxanthin, a natural carotenoid, against the cholestatic liver fibrosis induced by bile duct ligation (BDL). Toward this end, male rats were subjected to BDL and treated with astaxanthin for 35 days. Afterwards, their serum and liver biochemical factors were assessed. Also, histopathological and immunohistochemical analyses were performed to determine the fibrosis and the expression levels of alpha‐smooth muscle actin (α‐SMA) and transforming growth factor beta (TGF‐ß1) in the liver tissue. Based on the results, BDL caused a significant increase in liver enzyme levels, blood lipids, and bilirubin, while decreasing the activity of superoxide dismutase(SOD), catalase (CAT), and glutathione (GSH) enzymes. Also, in the BDL rats, hepatocyte necrosis, infiltration of inflammatory lymphocytes, and hyperplasia of bile ducts were detected, along with a significant increase in α‐SMA and TGF‐ß1 expression. Astaxanthin, however, significantly prevented the BDL's detrimental effects. In all, 10 mg/kg of this drug maintained the bilirubin and cholesterol serum levels of BDL rats at normal levels. It also reduced the liver enzymes' activity and serum lipids, while increasing the SOD, CAT, and GSH activity in BDL rats. The expression of α‐SMA and TGF‐ß1 in the BDL rats treated with 10 mg/kg of astaxanthin was moderate (in 34%–66% of cells) and no considerable cholestatic fibrosis was observed in this group. However, administrating the 20 mg/kg of astaxanthin was not effective in this regard. These findings showed that astaxanthin could considerably protect the liver from cholestatic damage by improving the biochemical features and regulating the expression of related proteins.

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Insights on Dietary Anticancer Products: Food Supplements, Prebiotics, and Probiotics

Elhawary,

Korany,

Eldahshan

et al. 2024

Interdisciplinary Cancer Research

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Pediococcus acidilactici isolated from traditional cheese as a potential probiotic with cytotoxic activity against doxorubicin-resistant MCF-7 cells

Cited by 4 publications

References 57 publications

Genomic characterization and antimicrobial activity of Pediococcus pentosaceus ST65ACC

Genomic characterization and antimicrobial activity of Pediococcus pentosaceus ST65ACC

Hepatoprotective effect of astaxanthin against cholestasis liver fibrosis induced by bile duct ligation in adult Wistar rats

Insights on Dietary Anticancer Products: Food Supplements, Prebiotics, and Probiotics

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